##gff-version 3
##sequence-region BBa_I739021 1 1746
BBa_I739021	.	engineered_region	1	821	.	+	0	ID=BBa_I739014;Name=BBa_I739014
BBa_I739021	.	ribosome_entry_site	92	103	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I739014
BBa_I739021	.	conserved_region	96	99	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I739021	.	promoter	1	83	.	+	0	ID=BBa_I739101;Name=BBa_I739101;Parent=BBa_I739014
BBa_I739021	.	non_covalent_binding_site	64	83	.	+	0	ID=annotation1946101;Name=TetR 1;Parent=BBa_I739101
BBa_I739021	.	promoter	1	35	.	+	0	ID=annotation1946100;Name=J23100;Parent=BBa_I739101
BBa_I739021	.	operator	44	83	.	+	0	ID=annotation1946257;Name=R0040 (2x TetR 1);Parent=BBa_I739101
BBa_I739021	.	sequence_feature	36	43	.	+	0	ID=annotation1946099;Name=Scar;Parent=BBa_I739101
BBa_I739021	.	non_covalent_binding_site	44	63	.	+	0	ID=annotation1946102;Name=TetR 1;Parent=BBa_I739101
BBa_I739021	.	CDS	110	796	.	+	0	ID=BBa_C0053;Name=BBa_C0053;Parent=BBa_I739014
BBa_I739021	.	stop_codon	791	796	.	+	0	ID=annotation1751;Name=stop;Parent=BBa_C0053
BBa_I739021	.	engineered_tag	797	821	.	+	0	ID=annotation2213993;Name=Help:Barcodes;Parent=BBa_C0053
BBa_I739021	.	engineered_region	110	796	.	+	0	ID=annotation7036;Name=BBa_C0053;Parent=BBa_C0053
BBa_I739021	.	CDS	110	757	.	+	0	ID=annotation1747;Name=cII p22;Parent=BBa_C0053
BBa_I739021	.	tag	758	790	.	+	0	ID=annotation1750;Name=LVA;Parent=BBa_C0053
BBa_I739021	.	engineered_region	830	1746	.	+	0	ID=BBa_I739005;Name=BBa_I739005
BBa_I739021	.	terminator	1618	1697	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I739005
BBa_I739021	.	engineered_region	1618	1697	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I739021	.	stem_loop	1629	1672	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I739021	.	terminator	1706	1746	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I739005
BBa_I739021	.	stop_codon	1739	1739	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I739021	.	polyA_site	1733	1746	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I739021	.	stem_loop	1713	1732	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I739021	.	engineered_region	1706	1746	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I739021	.	ribosome_entry_site	830	841	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I739005
BBa_I739021	.	conserved_region	834	837	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I739021	.	engineered_region	848	1609	.	+	0	ID=BBa_E0032;Name=BBa_E0032;Parent=BBa_I739005
BBa_I739021	.	stop_codon	1604	1609	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_I739021	.	tag	1565	1603	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_I739021	.	engineered_region	848	1609	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_I739021	.	sequence_alteration	1089	1089	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_I739021	.	CDS	848	1609	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
>BBa_I739021
ttgacggctagctcagtcctaggtacagtgctagctactagagtccctatcagtgatagagattccctatcagtgatag
agattactagagaaagaggagaaatactagatgaatacacaattgatgggtgagcgtattcgcgctcgaagaaaaaaac
tcaagattagacaagccgctcttggtaagatggtgggagtgtctaatgttgcaatatcgcaatgggagcgctcggagac
tgagccaaatggggagaacctgttggcactttcgaaggctcttcagtgctcccctgactatttgctgaaaggagattta
agccagacaaacgttgcctatcatagtaggcatgagccaagaggatcataccctcttatcagttgggtaagcgcagggc
aatggatggaagctgtagaaccttatcacaagcgcgcgatagagaactggcacgacaccactgtagattgttcagaaga
ttcattttggcttgatgtccaaggtgactctatgacagcaccggcagggttaagcattccagaaggaatgataattctg
gttgatcccgaagtcgaaccaagaaacggcaagctggttgttgcaaaattagaaggtgaaaacgaggccacattcaaaa
aattagttatggatgcaggccgaaagtttttaaaaccattaaacccacaatatccgatgatagaaatcaacggaaactg
caaaatcattggcgtagttgttgacgcaaaactcgcaaatcttccagctgcaaacgacgaaaactacgctttagtagct
taataacactgatagtgctagtgtagatcactactagagaaagaggagaaatactagatggtgagcaagggcgaggagc
tgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgaggg
cgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccacc
ctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagt
ccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggt
gaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctgggg
cacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaact
tcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacgg
ccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcac
atggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacg
acgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactggg
cctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctg
cgtttata