##gff-version 3
##sequence-region BBa_I739017 1 3542
BBa_I739017	.	engineered_region	1730	3542	.	+	0	ID=BBa_I739016;Name=BBa_I739016
BBa_I739017	.	engineered_region	2626	3542	.	+	0	ID=BBa_I739009;Name=BBa_I739009;Parent=BBa_I739016
BBa_I739017	.	engineered_region	2644	3405	.	+	0	ID=BBa_E0022;Name=BBa_E0022;Parent=BBa_I739009
BBa_I739017	.	tag	3362	3399	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
BBa_I739017	.	engineered_region	2644	3405	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
BBa_I739017	.	stop_codon	3400	3405	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_I739017	.	point_mutation	2712	2712	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_I739017	.	CDS	2644	3405	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_I739017	.	ribosome_entry_site	2626	2637	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I739009
BBa_I739017	.	conserved_region	2630	2633	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I739017	.	terminator	3414	3493	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I739009
BBa_I739017	.	stem_loop	3425	3468	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I739017	.	engineered_region	3414	3493	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I739017	.	terminator	3502	3542	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I739009
BBa_I739017	.	stem_loop	3509	3528	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I739017	.	stop_codon	3535	3535	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I739017	.	engineered_region	3502	3542	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I739017	.	polyA_site	3529	3542	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I739017	.	engineered_region	1730	2617	.	+	0	ID=BBa_I739008;Name=BBa_I739008;Parent=BBa_I739016
BBa_I739017	.	promoter	1730	1816	.	+	0	ID=BBa_I739103;Name=BBa_I739103;Parent=BBa_I739008
BBa_I739017	.	non_covalent_binding_site	1779	1797	.	+	0	ID=annotation1947821;Name=OR1;Parent=BBa_I739103
BBa_I739017	.	operator	1779	1816	.	+	0	ID=annotation1947823;Name=R0053 (2x OR1);Parent=BBa_I739103
BBa_I739017	.	non_covalent_binding_site	1798	1816	.	+	0	ID=annotation1947822;Name=OR1;Parent=BBa_I739103
BBa_I739017	.	promoter	1730	1777	.	+	0	ID=annotation1947820;Name=R0011;Parent=BBa_I739103
BBa_I739017	.	ribosome_entry_site	1825	1836	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I739008
BBa_I739017	.	conserved_region	1829	1832	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I739017	.	CDS	1843	2617	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_I739008
BBa_I739017	.	tag	2554	2586	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_I739017	.	CDS	1846	2553	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_I739017	.	engineered_tag	2593	2617	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_I739017	.	engineered_region	1	1721	.	+	0	ID=BBa_I739015;Name=BBa_I739015
BBa_I739017	.	engineered_region	844	1721	.	+	0	ID=BBa_E0430;Name=BBa_E0430;Parent=BBa_I739015
BBa_I739017	.	terminator	1681	1721	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0430
BBa_I739017	.	stem_loop	1688	1707	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I739017	.	stop_codon	1714	1714	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I739017	.	engineered_region	1681	1721	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I739017	.	polyA_site	1708	1721	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I739017	.	CDS	862	1584	.	+	0	ID=BBa_E0030;Name=BBa_E0030;Parent=BBa_E0430
BBa_I739017	.	ribosome_entry_site	844	855	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0430
BBa_I739017	.	conserved_region	848	851	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I739017	.	terminator	1593	1672	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0430
BBa_I739017	.	stem_loop	1604	1647	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I739017	.	engineered_region	1593	1672	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I739017	.	engineered_region	1	835	.	+	0	ID=BBa_I739004;Name=BBa_I739004;Parent=BBa_I739015
BBa_I739017	.	CDS	124	810	.	+	0	ID=BBa_C0053;Name=BBa_C0053;Parent=BBa_I739004
BBa_I739017	.	stop_codon	805	810	.	+	0	ID=annotation1751;Name=stop;Parent=BBa_C0053
BBa_I739017	.	tag	772	804	.	+	0	ID=annotation1750;Name=LVA;Parent=BBa_C0053
BBa_I739017	.	CDS	124	771	.	+	0	ID=annotation1747;Name=cII p22;Parent=BBa_C0053
BBa_I739017	.	engineered_tag	811	835	.	+	0	ID=annotation2213993;Name=Help:Barcodes;Parent=BBa_C0053
BBa_I739017	.	engineered_region	124	810	.	+	0	ID=annotation7036;Name=BBa_C0053;Parent=BBa_C0053
BBa_I739017	.	promoter	1	97	.	+	0	ID=BBa_I739102;Name=BBa_I739102;Parent=BBa_I739004
BBa_I739017	.	non_covalent_binding_site	78	97	.	+	0	ID=annotation1946932;Name=TetR 1;Parent=BBa_I739102
BBa_I739017	.	sequence_feature	50	57	.	+	0	ID=annotation1946931;Name=Scar;Parent=BBa_I739102
BBa_I739017	.	non_covalent_binding_site	58	77	.	+	0	ID=annotation1946934;Name=TetR 1;Parent=BBa_I739102
BBa_I739017	.	operator	58	97	.	+	0	ID=annotation1946933;Name=R0040 (2x TetR 1);Parent=BBa_I739102
BBa_I739017	.	promoter	1	49	.	+	0	ID=annotation1946930;Name=R0051;Parent=BBa_I739102
BBa_I739017	.	ribosome_entry_site	106	117	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I739004
BBa_I739017	.	conserved_region	110	113	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_I739017
taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagtccctatcagtgatagagattc
cctatcagtgatagagattactagagaaagaggagaaatactagatgaatacacaattgatgggtgagcgtattcgcgc
tcgaagaaaaaaactcaagattagacaagccgctcttggtaagatggtgggagtgtctaatgttgcaatatcgcaatgg
gagcgctcggagactgagccaaatggggagaacctgttggcactttcgaaggctcttcagtgctcccctgactatttgc
tgaaaggagatttaagccagacaaacgttgcctatcatagtaggcatgagccaagaggatcataccctcttatcagttg
ggtaagcgcagggcaatggatggaagctgtagaaccttatcacaagcgcgcgatagagaactggcacgacaccactgta
gattgttcagaagattcattttggcttgatgtccaaggtgactctatgacagcaccggcagggttaagcattccagaag
gaatgataattctggttgatcccgaagtcgaaccaagaaacggcaagctggttgttgcaaaattagaaggtgaaaacga
ggccacattcaaaaaattagttatggatgcaggccgaaagtttttaaaaccattaaacccacaatatccgatgatagaa
atcaacggaaactgcaaaatcattggcgtagttgttgacgcaaaactcgcaaatcttccagctgcaaacgacgaaaact
acgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaagaggagaaatactagatggtgag
caagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagc
gtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccg
tgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgca
cgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaag
acccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacg
gcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacgg
catcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacc
cccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacg
agaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagta
ataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtc
ggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgag
cggataacaattgacattgtgagcggataacaagatactaatttaagtgttctttaattatttaagtgttctttaattt
actagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgc
cttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggc
agtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaa
agttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtca
cttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttacca
aaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattc
catgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgag
ccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgt
ttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctag
tcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctag
tgtagatcgctactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgccca
tcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacgg
caagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctgg
ggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacg
tccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccct
ggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactac
atcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcg
aggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaa
ccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtg
accgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtag
cttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtt
tgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata