##gff-version 3
##sequence-region BBa_I739012 1 2338
BBa_I739012	.	engineered_region	1	1351	.	+	0	ID=BBa_I739002;Name=BBa_I739002
BBa_I739012	.	terminator	1223	1351	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_I739002
BBa_I739012	.	terminator	1311	1351	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I739012	.	stem_loop	1318	1337	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I739012	.	stop_codon	1344	1344	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I739012	.	polyA_site	1338	1351	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I739012	.	engineered_region	1311	1351	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I739012	.	terminator	1223	1302	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I739012	.	engineered_region	1223	1302	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I739012	.	stem_loop	1234	1277	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I739012	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I739002
BBa_I739012	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I739012	.	CDS	62	1189	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_I739002
BBa_I739012	.	engineered_tag	1190	1214	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_I739012	.	CDS	62	1189	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_I739012	.	engineered_region	62	1189	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_I739012	.	tag	1151	1189	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_I739012	.	promoter	1	35	.	+	0	ID=BBa_J23105;Name=BBa_J23105;Parent=BBa_I739002
BBa_I739012	.	engineered_region	1360	2338	.	+	0	ID=BBa_I739003;Name=BBa_I739003
BBa_I739012	.	CDS	1421	2176	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I739003
BBa_I739012	.	engineered_region	1421	2176	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_I739012	.	point_mutation	1912	1912	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_I739012	.	point_mutation	1594	1594	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_I739012	.	engineered_tag	2177	2201	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_I739012	.	engineered_region	1421	1422	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_I739012	.	CDS	1421	2170	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_I739012	.	ribosome_entry_site	1403	1414	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I739003
BBa_I739012	.	conserved_region	1407	1410	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I739012	.	terminator	2210	2338	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_I739003
BBa_I739012	.	terminator	2298	2338	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I739012	.	stem_loop	2305	2324	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I739012	.	stop_codon	2331	2331	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I739012	.	polyA_site	2325	2338	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I739012	.	engineered_region	2298	2338	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I739012	.	terminator	2210	2289	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I739012	.	engineered_region	2210	2289	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I739012	.	stem_loop	2221	2264	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I739012	.	promoter	1360	1394	.	+	0	ID=BBa_J23105;Name=BBa_J23105;Parent=BBa_I739003
>BBa_I739012
tttacggctagctcagtcctaggtactatgctagctactagagaaagaggagaaatactagatggtgaatgtgaaacca
gtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacg
tttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaact
ggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcg
attaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaag
cggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgc
tgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttc
tcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcc
cattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagc
ggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccact
gcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcgg
atatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattt
tcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttg
cccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcat
taatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagctta
ataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactggg
cctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctg
cgtttatatactagagtttacggctagctcagtcctaggtactatgctagctactagagaaagaggagaaatactagat
gaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaa
tgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaat
ctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcc
tatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatct
ccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggct
tcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacatacc
attaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaa
agagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagc
gtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaat
tttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagcca
ggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcta
ctagagtcacactggctcaccttcgggtgggcctttctgcgtttata