##gff-version 3
##sequence-region BBa_I737010 1 2805
BBa_I737010	.	engineered_region	1124	1922	.	+	0	ID=BBa_S01414;Name=BBa_S01414
BBa_I737010	.	ribosome_entry_site	1124	1135	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S01414
BBa_I737010	.	conserved_region	1128	1131	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I737010	.	CDS	1142	1897	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_S01414
BBa_I737010	.	engineered_region	1142	1897	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_I737010	.	engineered_region	1142	1143	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_I737010	.	engineered_tag	1898	1922	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_I737010	.	point_mutation	1633	1633	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_I737010	.	point_mutation	1315	1315	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_I737010	.	CDS	1142	1891	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_I737010	.	promoter	1	108	.	+	0	ID=BBa_R0084;Name=BBa_R0084
BBa_I737010	.	non_covalent_binding_site	51	68	.	+	0	ID=annotation301313;Name=F3 OmpR;Parent=BBa_R0084
BBa_I737010	.	non_covalent_binding_site	31	48	.	+	0	ID=annotation301309;Name=F2 OmpR;Parent=BBa_R0084
BBa_I737010	.	promoter	73	78	.	+	0	ID=annotation301317;Name=-35;Parent=BBa_R0084
BBa_I737010	.	non_covalent_binding_site	11	28	.	+	0	ID=annotation301308;Name=F1 OmpR;Parent=BBa_R0084
BBa_I737010	.	promoter	88	93	.	+	0	ID=annotation301318;Name=-10;Parent=BBa_R0084
BBa_I737010	.	engineered_region	1931	2805	.	+	0	ID=BBa_I13504;Name=BBa_I13504
BBa_I737010	.	terminator	2765	2805	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13504
BBa_I737010	.	stop_codon	2798	2798	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I737010	.	polyA_site	2792	2805	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I737010	.	stem_loop	2772	2791	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I737010	.	engineered_region	2765	2805	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I737010	.	ribosome_entry_site	1931	1942	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13504
BBa_I737010	.	conserved_region	1935	1938	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I737010	.	CDS	1949	2668	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_I13504
BBa_I737010	.	CDS	1949	2668	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_I737010	.	terminator	2677	2756	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13504
BBa_I737010	.	stem_loop	2688	2731	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I737010	.	engineered_region	2677	2756	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I737010	.	engineered_region	117	1115	.	+	0	ID=BBa_A340620;Name=BBa_A340620
BBa_I737010	.	terminator	1012	1052	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_A340620
BBa_I737010	.	stop_codon	1045	1045	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I737010	.	polyA_site	1039	1052	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I737010	.	stem_loop	1019	1038	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I737010	.	engineered_region	1012	1052	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I737010	.	ribosome_entry_site	117	128	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_A340620
BBa_I737010	.	conserved_region	121	124	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I737010	.	CDS	135	890	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_A340620
BBa_I737010	.	engineered_region	135	890	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_I737010	.	engineered_region	135	136	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_I737010	.	engineered_tag	891	915	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_I737010	.	point_mutation	626	626	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_I737010	.	point_mutation	308	308	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_I737010	.	CDS	135	884	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_I737010	.	promoter	1061	1115	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_A340620
BBa_I737010	.	start_codon	1113	1113	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_I737010	.	promoter	1080	1085	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_I737010	.	engineered_region	1061	1115	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_I737010	.	promoter	1102	1107	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_I737010	.	non_covalent_binding_site	1061	1080	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_I737010	.	terminator	924	1003	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_A340620
BBa_I737010	.	stem_loop	935	978	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I737010	.	engineered_region	924	1003	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_I737010
cttaaattttacttttggttacatattttttctttttgaaaccaaatctttatctttgtagcactttcacggtagcgaa
acgttagtttgaatggaaagatgcctgcatactagagaaagaggagaaatactagatgaaaaacataaatgccgacgac
acatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaa
tggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataa
ttaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactcc
aatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcga
aaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcaca
ttcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagtt
gataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgt
gggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaac
caatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgc
ccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggc
tcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacct
tcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatag
tcgaataaatactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataa
aattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattat
ttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggc
aatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattg
gaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcact
gggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactata
tagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaa
tatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagc
tcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactca
atacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaata
acactgatagtgctagtgtagatcactactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcact
ggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtg
atgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcac
tactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatg
cccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttg
aaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaatt
ggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaatt
agacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcc
ttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtcct
tcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatca
aataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagt
cacactggctcaccttcgggtgggcctttctgcgtttata