##gff-version 3
##sequence-region BBa_I737009 1 5332
BBa_I737009	.	engineered_region	1000	5332	.	+	0	ID=BBa_M30109;Name=BBa_M30109
BBa_I737009	.	ribosome_entry_site	1915	1926	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_M30109
BBa_I737009	.	conserved_region	1919	1922	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I737009	.	terminator	2804	2844	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_M30109
BBa_I737009	.	stem_loop	2811	2830	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I737009	.	stop_codon	2837	2837	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I737009	.	polyA_site	2831	2844	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I737009	.	engineered_region	2804	2844	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I737009	.	CDS	2933	5170	.	+	0	ID=BBa_I15010;Name=BBa_I15010;Parent=BBa_M30109
BBa_I737009	.	engineered_tag	5171	5195	.	+	0	ID=annotation2214031;Name=Help:Barcodes;Parent=BBa_I15010
BBa_I737009	.	CDS	2933	5170	.	+	0	ID=annotation1891613;Name=Cph8;Parent=BBa_I15010
BBa_I737009	.	CDS	1933	2682	.	+	0	ID=BBa_I15009;Name=BBa_I15009;Parent=BBa_M30109
BBa_I737009	.	CDS	1933	2682	.	+	0	ID=annotation1891612;Name=PcyA;Parent=BBa_I15009
BBa_I737009	.	engineered_tag	2683	2707	.	+	0	ID=annotation2214032;Name=Help:Barcodes;Parent=BBa_I15009
BBa_I737009	.	terminator	2716	2795	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_M30109
BBa_I737009	.	stem_loop	2727	2770	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I737009	.	engineered_region	2716	2795	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I737009	.	terminator	5204	5283	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_M30109
BBa_I737009	.	stem_loop	5215	5258	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I737009	.	engineered_region	5204	5283	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I737009	.	ribosome_entry_site	1138	1149	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_M30109
BBa_I737009	.	conserved_region	1142	1145	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I737009	.	terminator	5292	5332	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_M30109
BBa_I737009	.	stem_loop	5299	5318	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I737009	.	stop_codon	5325	5325	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I737009	.	polyA_site	5319	5332	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I737009	.	engineered_region	5292	5332	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I737009	.	promoter	1000	1129	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_M30109
BBa_I737009	.	ribosome_entry_site	2915	2926	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_M30109
BBa_I737009	.	conserved_region	2919	2922	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I737009	.	CDS	1156	1881	.	+	0	ID=BBa_I15008;Name=BBa_I15008;Parent=BBa_M30109
BBa_I737009	.	engineered_tag	1882	1906	.	+	0	ID=annotation2214026;Name=Help:Barcodes;Parent=BBa_I15008
BBa_I737009	.	promoter	2853	2906	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_M30109
BBa_I737009	.	engineered_region	2853	2906	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I737009	.	promoter	2872	2877	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I737009	.	promoter	2895	2900	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I737009	.	non_covalent_binding_site	2853	2871	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I737009	.	non_covalent_binding_site	2878	2896	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I737009	.	engineered_region	1	991	.	+	0	ID=BBa_I737008;Name=BBa_I737008
BBa_I737009	.	ribosome_entry_site	117	128	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I737008
BBa_I737009	.	conserved_region	121	124	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I737009	.	terminator	951	991	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I737008
BBa_I737009	.	stem_loop	958	977	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I737009	.	stop_codon	984	984	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I737009	.	polyA_site	978	991	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I737009	.	engineered_region	951	991	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I737009	.	CDS	135	854	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_I737008
BBa_I737009	.	CDS	135	854	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_I737009	.	terminator	863	942	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I737008
BBa_I737009	.	stem_loop	874	917	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I737009	.	engineered_region	863	942	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I737009	.	promoter	1	108	.	+	0	ID=BBa_R0084;Name=BBa_R0084;Parent=BBa_I737008
BBa_I737009	.	non_covalent_binding_site	51	68	.	+	0	ID=annotation301313;Name=F3 OmpR;Parent=BBa_R0084
BBa_I737009	.	non_covalent_binding_site	11	28	.	+	0	ID=annotation301308;Name=F1 OmpR;Parent=BBa_R0084
BBa_I737009	.	non_covalent_binding_site	31	48	.	+	0	ID=annotation301309;Name=F2 OmpR;Parent=BBa_R0084
BBa_I737009	.	promoter	88	93	.	+	0	ID=annotation301318;Name=-10;Parent=BBa_R0084
BBa_I737009	.	promoter	73	78	.	+	0	ID=annotation301317;Name=-35;Parent=BBa_R0084
>BBa_I737009
cttaaattttacttttggttacatattttttctttttgaaaccaaatctttatctttgtagcactttcacggtagcgaa
acgttagtttgaatggaaagatgcctgcatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttc
actggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaag
gtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgt
cactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgcc
atgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagt
ttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaa
attggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaa
attagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctg
tccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggt
ccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggca
tcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactag
agtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacattgattatttgcacggcgtcacact
ttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttct
ccataccgtttttttgggctagctactagagaaagaggagaaatactagatgagtgtcaacttagcttcccagttgcgg
gaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaagggcgttgtcgagaaaa
attcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggcaaaatttaaggacca
tcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggc
tccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacgg
cccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgc
ccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaa
aatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacg
cctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacag
cctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctaataacgctgatagtgctag
tgtagatcgctactagagaaagaggagaaatactagatggccgtcactgatttaagtttgaccaattcttccctgatgc
ctacgttgaacccgatgattcaacagttggccctggcgatcgccgctagttggcaaagtttacccctcaagccctatca
attgccggaggatttgggctacgtagaaggccgcctggaaggggaaaagttagtgattgaaaatcggtgctaccaaacg
ccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaagggttggatattctccactgtgtaatgtttcctg
agcctttatacggtctacctttgtttggctgtgacattgtggccggccccggtggagtaagtgcggctattgcggatct
atcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatcattggcagagctaggccagccagaatttgag
caacaacgggaattgcccccctggggagaaatattttctgaatattgtttattcatccgtcccagcaatgtcactgaag
aagaaagatttgtacaaagggtagtggactttttgcaaattcattgtcaccaatccatcgttgccgaacccttgtctga
agctcaaactttggagcaccgtcaggggcaaattcattactgccaacaacaacagaaaaatgataaaacccgtcgggta
ctggaaaaagcttttggggaagcttgggcggaacggtatatgagccaagtcttatttgatgttatccaataataactct
gatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgt
tttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
tactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggag
aaatactagatggccaccaccgtacaactcagcgaccaatccctccgtcagctagaaaccctcgccatccacaccgccc
acctgattcagccccacggtttagtggtggtcctgcaagaaccagacctcaccatcagccaaattagcgccaactgcac
cggcattttagggcgatcgccagaggatttgttgggcagaaccctaggggaagtgtttgatagctttcagattgatccc
atccagagtcgcctaacggccggacaaatcagcagcctcaaccccagtaaactttgggcgcgggtcatgggggacgact
ttgtcatttttgacggggtttttcatcgcaacagtgacggtttattggtatgtgaactcgagccagcctacacttccga
taatctgcccttcctcggtttttatcacatggccaacgctgccctgaatcggttgcgccaacaagctaatctacgggat
ttctacgatgttattgtcgaagaagtccgccgtatgactggctttgaccgggtgatgctataccgctttgatgaaaata
accacggtgatgtcattgccgaagataaacgggatgatatggaaccctatttgggcctgcactatcccgaatcggatat
tccccaacccgcccgtcggctatttatccacaaccccattcgagtaattcccgatgtttatggtgtggcggtgcccctg
accccagcggttaaccccagcaccaaccgagcggtggatttaacagaatccattctgcgcagtgcgtaccattgccact
tgacctatctgaaaaatatgggggtaggagcgtctttaaccatttccctaattaaggacggccatctctgggggctcat
tgcctgccaccatcaaacccccaaagtaattccctttgaactgcgtaaagcctgcgaattttttggtcgggtggtgttt
agcaacatttccgcccaggaagatacggaaaccttcgattaccgggtgcagctggcggagcatgaagcggttttattgg
acaaaatgaccacggcggcggattttgtcgaaggattaactaatcatcccgatcgcctgttgggattaacgggctccca
gggggcggccatttgctttggggaaaaattgattttagtaggggaaaccccggacgagaaagcagtgcaatatttactg
caatggttggagaatcgggaagtgcaagacgttttcttcacctcttccctctcacaaatttatcctgatgcagtgaatt
ttaaatccgtggccagtggcttattggccattcccattgcccgtcacaactttttgctctggtttcgccctgaagtgtt
gcaaacggttaattggggcggtgacccaaatcatgcttacgaagctacccaggaagacggtaaaatcgagctccatccc
cgccaatcctttgacctctggaaagaaattgtccgactccaatctttgccctggcaatcggtggaaatccaaagtgccc
tggccctgaaaaaggcgatcgtcaacctcattttgcgccaggcagaagaattgcatatggcggctggtgttaagcaact
ggcggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgact
gagatgatgagcgagcaggatggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgccatcattgagc
agtttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtgat
tgctgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgctg
tcgatcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatggcaatggctgggtcaaagtcagcagcggaa
cggagccgaatcgcgcctggttccaggtggaagatgacggtccgggaattgcgccggaacaacgtaagcacctgttcca
gccgtttgtccgcggcgacagtgcgcgcaccattagcggcacgggattagggctggcaattgtgcagcgtatcgtggat
aaccataacgggatgctggagcttggcaccagcgagcggggcgggctttccattcgcgcctggctgccagtgccggtaa
cgcgggcgcagggcatgacaaaagaagggtaataactctgatagtgctagtgtagatctctactagagccaggcatcaa
ataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtc
acactggctcaccttcgggtgggcctttctgcgtttata