##gff-version 3
##sequence-region BBa_I732923 1 5682
BBa_I732923	.	engineered_region	1	1373	.	+	0	ID=BBa_I732907;Name=BBa_I732907
BBa_I732923	.	terminator	1245	1324	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I732907
BBa_I732923	.	stem_loop	1256	1299	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I732923	.	engineered_region	1245	1324	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I732923	.	terminator	1333	1373	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I732907
BBa_I732923	.	polyA_site	1360	1373	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I732923	.	engineered_region	1333	1373	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I732923	.	stem_loop	1340	1359	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I732923	.	stop_codon	1366	1366	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I732923	.	CDS	151	1236	.	+	0	ID=BBa_I732112;Name=BBa_I732112;Parent=BBa_I732907
BBa_I732923	.	promoter	1	124	.	+	0	ID=BBa_I732205;Name=BBa_I732205;Parent=BBa_I732907
BBa_I732923	.	ribosome_entry_site	133	144	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I732907
BBa_I732923	.	conserved_region	137	140	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I732923	.	engineered_region	1382	5682	.	+	0	ID=BBa_I732922;Name=BBa_I732922
BBa_I732923	.	engineered_region	2278	3518	.	+	0	ID=BBa_I732822;Name=BBa_I732822;Parent=BBa_I732922
BBa_I732923	.	CDS	2296	3381	.	+	0	ID=BBa_I732102;Name=BBa_I732102;Parent=BBa_I732822
BBa_I732923	.	terminator	3390	3469	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I732822
BBa_I732923	.	stem_loop	3401	3444	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I732923	.	engineered_region	3390	3469	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I732923	.	terminator	3478	3518	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I732822
BBa_I732923	.	polyA_site	3505	3518	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I732923	.	engineered_region	3478	3518	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I732923	.	stem_loop	3485	3504	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I732923	.	stop_codon	3511	3511	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I732923	.	ribosome_entry_site	2278	2289	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I732822
BBa_I732923	.	conserved_region	2282	2285	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I732923	.	engineered_region	4579	5682	.	+	0	ID=BBa_I732815;Name=BBa_I732815;Parent=BBa_I732922
BBa_I732923	.	CDS	4597	5682	.	+	0	ID=BBa_I732115;Name=BBa_I732115;Parent=BBa_I732815
BBa_I732923	.	ribosome_entry_site	4579	4590	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I732815
BBa_I732923	.	conserved_region	4583	4586	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I732923	.	engineered_region	3527	4570	.	+	0	ID=BBa_J09855;Name=BBa_J09855;Parent=BBa_I732922
BBa_I732923	.	promoter	3527	3563	.	+	0	ID=BBa_I14032;Name=BBa_I14032;Parent=BBa_J09855
BBa_I732923	.	operator	3527	3563	.	+	0	ID=annotation1028342;Name=P(Lac) IQ;Parent=BBa_I14032
BBa_I732923	.	promoter	3529	3534	.	+	0	ID=annotation1028343;Name=-35;Parent=BBa_I14032
BBa_I732923	.	promoter	3552	3557	.	+	0	ID=annotation1028344;Name=-10;Parent=BBa_I14032
BBa_I732923	.	promoter	4516	4570	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_J09855
BBa_I732923	.	engineered_region	4516	4570	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_I732923	.	promoter	4535	4540	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_I732923	.	promoter	4557	4562	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_I732923	.	start_codon	4568	4568	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_I732923	.	non_covalent_binding_site	4516	4535	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_I732923	.	engineered_region	3572	4507	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_J09855
BBa_I732923	.	terminator	4379	4458	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_I732923	.	stem_loop	4390	4433	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I732923	.	engineered_region	4379	4458	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I732923	.	terminator	4467	4507	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_I732923	.	polyA_site	4494	4507	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I732923	.	engineered_region	4467	4507	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I732923	.	stem_loop	4474	4493	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I732923	.	stop_codon	4500	4500	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I732923	.	ribosome_entry_site	3572	3583	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_I732923	.	conserved_region	3576	3579	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I732923	.	CDS	3590	4345	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_I732923	.	CDS	3590	4339	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_I732923	.	engineered_region	3590	4345	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_I732923	.	engineered_tag	4346	4370	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_I732923	.	point_mutation	3763	3763	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_I732923	.	engineered_region	3590	3591	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_I732923	.	point_mutation	4081	4081	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_I732923	.	engineered_region	1382	2269	.	+	0	ID=BBa_I732086;Name=BBa_I732086;Parent=BBa_I732922
BBa_I732923	.	terminator	2167	2207	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I732086
BBa_I732923	.	polyA_site	2194	2207	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I732923	.	engineered_region	2167	2207	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I732923	.	stem_loop	2174	2193	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I732923	.	stop_codon	2200	2200	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I732923	.	terminator	2079	2158	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I732086
BBa_I732923	.	stem_loop	2090	2133	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I732923	.	engineered_region	2079	2158	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I732923	.	engineered_region	1382	2070	.	+	0	ID=BBa_I732084;Name=BBa_I732084;Parent=BBa_I732086
BBa_I732923	.	promoter	2216	2269	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_I732086
BBa_I732923	.	engineered_region	2216	2269	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I732923	.	non_covalent_binding_site	2216	2234	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I732923	.	non_covalent_binding_site	2241	2259	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I732923	.	promoter	2235	2240	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I732923	.	promoter	2258	2263	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
>BBa_I732923
tacccacaacccaattcgagaccggaactcgattgtatctgtagtgctttagtagtggagtttacactttatgcttccg
gctcgtataatgtgtggaattttgagcgctcaaaattggatccggtactagagaaagaggagaaatactagatgaaacc
agtaacgttatacgatgtcgcagagtatgccggtgtctcttgtgcgaccgtttcccgcgtggtgaaccaggccagccac
gtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaac
tggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggc
gattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaa
gcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattg
ctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattatttt
ctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggc
ccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatag
cggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccac
tgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcg
gatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggatt
ttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgtt
gcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattca
ttaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataatactagagccaggcatcaaataaaacga
aaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggct
caccttcgggtgggcctttctgcgtttatatactagagtggtgcaaaacctttgcggtatggcatgatagcgcctacta
gagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgagg
tcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaa
aaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggg
gaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaag
tacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggttt
ttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcat
caagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttg
atcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtga
aagtgggtcctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttat
ctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatacta
gagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaata
ctagatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctctcatcagaccgtttccaacgtggtgaac
caggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcg
tggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgca
aattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtc
gaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgacc
aggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaa
cagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcg
ctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaa
ttcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgaggg
catcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctg
cgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccacca
tcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaaggg
caatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcg
ttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataatactagagccaggcat
caaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactaga
gtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtggtgcaaaacctttcgcggtatggcatg
atagcgcctactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaa
attaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatt
tactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggca
atattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattgg
aatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactg
ggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatat
agatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaat
atagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagct
cttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaa
tacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataa
cactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctt
tcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtt
tatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaag
aggagaaatactagatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctctcgccaaaccgtttcccg
cgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacatt
cccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacg
cgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacg
aagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccg
ctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccaga
cacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcacca
gcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcact
cgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgc
tgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccga
gtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccg
ttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccagg
cggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctc
tccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataa