##gff-version 3
##sequence-region BBa_I732745 1 1294
BBa_I732745	.	engineered_region	174	1294	.	+	0	ID=BBa_I732092;Name=BBa_I732092
BBa_I732745	.	ribosome_entry_site	434	445	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I732092
BBa_I732745	.	conserved_region	438	441	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I732745	.	CDS	452	1157	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I732092
BBa_I732745	.	engineered_tag	1133	1157	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_I732745	.	CDS	452	1126	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_I732745	.	engineered_region	174	425	.	+	0	ID=BBa_I732018;Name=BBa_I732018;Parent=BBa_I732092
BBa_I732745	.	ribosome_entry_site	174	185	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I732018
BBa_I732745	.	conserved_region	178	181	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I732745	.	CDS	192	425	.	+	0	ID=BBa_I732006;Name=BBa_I732006;Parent=BBa_I732018
BBa_I732745	.	CDS	192	425	.	+	0	ID=annotation1936904;Name=lacZ;Parent=BBa_I732006
BBa_I732745	.	start_codon	192	194	.	+	0	ID=annotation1936905;Name=START;Parent=BBa_I732006
BBa_I732745	.	stop_codon	420	425	.	+	0	ID=annotation1936906;Name=STOP;Parent=BBa_I732006
BBa_I732745	.	terminator	1166	1294	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_I732092
BBa_I732745	.	terminator	1254	1294	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I732745	.	polyA_site	1281	1294	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I732745	.	engineered_region	1254	1294	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I732745	.	stop_codon	1287	1287	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I732745	.	stem_loop	1261	1280	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I732745	.	terminator	1166	1245	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I732745	.	stem_loop	1177	1220	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I732745	.	engineered_region	1166	1245	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I732745	.	promoter	1	165	.	+	0	ID=BBa_I732400;Name=BBa_I732400
>BBa_I732745
tacccacaacccaattcgagaccggaactcgattgtatctgtagtgctttagtagtggagtttacactttatgcttccg
gctcgtataatgtgtggaacatcccctttatctatcgcctgtgcccactacatcaagccaaattaaacaggattaacag
gatccggtactagagaaagaggagaaatactagatgaccatgattacggattcactggccgtcgttttacaacgtcgtg
actgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaaga
ggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaa
gcggtgccggaaagctggctggagtaataatactagagaaagaggagaaatactagatggcttcctccgaagacgttat
caaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaa
ggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcc
tgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtcctt
cccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctg
caagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaa
ccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaa
actgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgct
tacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaag
gtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacga
aaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggct
caccttcgggtgggcctttctgcgtttata