##gff-version 3
##sequence-region BBa_I732730 1 1280
BBa_I732730	.	ribosome_entry_site	28	39	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I732730	.	conserved_region	32	35	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I732730	.	engineered_region	146	1280	.	+	0	ID=BBa_I732093;Name=BBa_I732093
BBa_I732730	.	CDS	164	397	.	+	0	ID=BBa_I732006;Name=BBa_I732006;Parent=BBa_I732093
BBa_I732730	.	start_codon	164	166	.	+	0	ID=annotation1936905;Name=START;Parent=BBa_I732006
BBa_I732730	.	stop_codon	392	397	.	+	0	ID=annotation1936906;Name=STOP;Parent=BBa_I732006
BBa_I732730	.	CDS	164	397	.	+	0	ID=annotation1936904;Name=lacZ;Parent=BBa_I732006
BBa_I732730	.	terminator	1152	1231	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I732093
BBa_I732730	.	stem_loop	1163	1206	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I732730	.	engineered_region	1152	1231	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I732730	.	ribosome_entry_site	146	157	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I732093
BBa_I732730	.	conserved_region	150	153	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I732730	.	terminator	1240	1280	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I732093
BBa_I732730	.	stop_codon	1273	1273	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I732730	.	engineered_region	1240	1280	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I732730	.	stem_loop	1247	1266	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I732730	.	polyA_site	1267	1280	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I732730	.	ribosome_entry_site	406	417	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I732093
BBa_I732730	.	conserved_region	410	413	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I732730	.	CDS	424	1143	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_I732093
BBa_I732730	.	CDS	424	1143	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_I732730	.	CDS	46	279	.	+	0	ID=BBa_I732006;Name=BBa_I732006
BBa_I732730	.	start_codon	46	48	.	+	0	ID=annotation1936905;Name=START;Parent=BBa_I732006
BBa_I732730	.	stop_codon	274	279	.	+	0	ID=annotation1936906;Name=STOP;Parent=BBa_I732006
BBa_I732730	.	CDS	46	279	.	+	0	ID=annotation1936904;Name=lacZ;Parent=BBa_I732006
BBa_I732730	.	ribosome_entry_site	288	299	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I732730	.	conserved_region	292	295	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I732730	.	promoter	1	137	.	+	0	ID=BBa_I732405;Name=BBa_I732405
BBa_I732730	.	stop_codon	1141	1141	.	+	0	ID=annotation1944423;Name=stop;Parent=BBa_I732405
BBa_I732730	.	engineered_region	288	299	.	+	0	ID=annotation1944414;Name=BBa_B0034;Parent=BBa_I732405
BBa_I732730	.	start_codon	46	48	.	+	0	ID=annotation1944409;Name=START;Parent=BBa_I732405
BBa_I732730	.	polyA_site	1135	1148	.	+	0	ID=annotation1944422;Name=polya;Parent=BBa_I732405
BBa_I732730	.	engineered_region	28	39	.	+	0	ID=annotation1944408;Name=BBa_B0034;Parent=BBa_I732405
BBa_I732730	.	conserved_region	292	295	.	+	0	ID=annotation1944413;Name=conserved;Parent=BBa_I732405
BBa_I732730	.	CDS	306	980	.	+	0	ID=annotation1944415;Name=mrfp1;Parent=BBa_I732405
BBa_I732730	.	engineered_region	987	1011	.	+	0	ID=annotation1944417;Name=barcode;Parent=BBa_I732405
BBa_I732730	.	engineered_region	46	279	.	+	0	ID=annotation1944412;Name=BBa_I732006;Parent=BBa_I732405
BBa_I732730	.	engineered_region	1108	1148	.	+	0	ID=annotation1944420;Name=BBa_B0012;Parent=BBa_I732405
BBa_I732730	.	engineered_region	1	19	.	+	0	ID=annotation1944406;Name=BBa_I732007;Parent=BBa_I732405
BBa_I732730	.	conserved_region	32	35	.	+	0	ID=annotation1944407;Name=conserved;Parent=BBa_I732405
BBa_I732730	.	stop_codon	274	279	.	+	0	ID=annotation1944411;Name=STOP;Parent=BBa_I732405
BBa_I732730	.	sequence_feature	12	17	.	+	0	ID=annotation1944405;Name=BamHI;Parent=BBa_I732405
BBa_I732730	.	CDS	46	279	.	+	0	ID=annotation1944410;Name=lacZ;Parent=BBa_I732405
BBa_I732730	.	sequence_feature	3	8	.	+	0	ID=annotation1944404;Name=BglII;Parent=BBa_I732405
BBa_I732730	.	stem_loop	1031	1074	.	+	0	ID=annotation1944419;Name=stem_loop;Parent=BBa_I732405
BBa_I732730	.	engineered_region	1020	1099	.	+	0	ID=annotation1944418;Name=BBa_B0010;Parent=BBa_I732405
BBa_I732730	.	stem_loop	1115	1134	.	+	0	ID=annotation1944421;Name=T7 TE;Parent=BBa_I732405
BBa_I732730	.	engineered_region	306	986	.	+	0	ID=annotation1944416;Name=BBa_E1010;Parent=BBa_I732405
BBa_I732730	.	terminator	1108	1148	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I732730	.	stop_codon	1141	1141	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I732730	.	engineered_region	1108	1148	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I732730	.	stem_loop	1115	1134	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I732730	.	polyA_site	1135	1148	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I732730	.	terminator	1020	1099	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I732730	.	stem_loop	1031	1074	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I732730	.	engineered_region	1020	1099	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_I732730
aattgtgagcgctcacaattttagtagtggagtttacactttatgcttccggctcgtataatgtgtggaacatcccctt
tatctatcgcctgtgcccactacatcaagccaaattaaacaggattaacaggatccggtactagagaaagaggagaaat
actagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaact
taatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacag
ttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtaat
aatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaa
ttagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttaccc
ttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatg
ctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaaga
actatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaa
tcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaa
tgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagc
gttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgt
ccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgg
gattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaa
agactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtggg
cctttctgcgtttata