##gff-version 3
##sequence-region BBa_I732075 1 4042
BBa_I732075	.	ribosome_entry_site	3129	3140	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I732075	.	conserved_region	3133	3136	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I732075	.	CDS	46	3120	.	+	0	ID=BBa_I732005;Name=BBa_I732005
BBa_I732075	.	CDS	46	3120	.	+	0	ID=annotation1936870;Name=lacZ;Parent=BBa_I732005
BBa_I732075	.	point_mutation	3069	3069	.	+	0	ID=annotation1936869;Name=C->T;Parent=BBa_I732005
BBa_I732075	.	start_codon	46	48	.	+	0	ID=annotation1936871;Name=START;Parent=BBa_I732005
BBa_I732075	.	stop_codon	3118	3120	.	+	0	ID=annotation1936872;Name=STOP;Parent=BBa_I732005
BBa_I732075	.	terminator	4002	4042	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I732075	.	engineered_region	4002	4042	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I732075	.	polyA_site	4029	4042	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I732075	.	stem_loop	4009	4028	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I732075	.	stop_codon	4035	4035	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I732075	.	sequence_feature	1	19	.	+	0	ID=BBa_I732007;Name=BBa_I732007
BBa_I732075	.	sequence_feature	12	17	.	+	0	ID=annotation1936908;Name=BamHI;Parent=BBa_I732007
BBa_I732075	.	sequence_feature	3	8	.	+	0	ID=annotation1936907;Name=BglII;Parent=BBa_I732007
BBa_I732075	.	ribosome_entry_site	28	39	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I732075	.	conserved_region	32	35	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I732075	.	engineered_region	3147	3905	.	+	0	ID=BBa_E0044;Name=BBa_E0044
BBa_I732075	.	terminator	3914	3993	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I732075	.	engineered_region	3914	3993	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I732075	.	stem_loop	3925	3968	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_I732075
gaagatcttccggatccggtactagagaaagaggagaaatactagatgaccatgattacggattcactggccgtcgttt
tacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcg
taatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggttt
ccggcaccagaagcggtgccggaaagctggctggagtgcgatcttcctgaggccgatactgtcgtcgtcccctcaaact
ggcagatgcacggttacgatgcgcccatctacaccaacgtgacctatcccattacggtcaatccgccgtttgttcccac
ggagaatccgacgggttgttactcgctcacatttaatgttgatgaaagctggctacaggaaggccagacgcgaattatt
tttgatggcgttaactcggcgtttcatctgtggtgcaacgggcgctgggtcggttacggccaggacagtcgtttgccgt
ctgaatttgacctgagcgcatttttacgcgccggagaaaaccgcctcgcggtgatggtgctgcgctggagtgacggcag
ttatctggaagatcaggatatgtggcggatgagcggcattttccgtgacgtctcgttgctgcataaaccgactacacaa
atcagcgatttccatgttgccactcgctttaatgatgatttcagccgcgctgtactggaggctgaagttcagatgtgcg
gcgagttgcgtgactacctacgggtaacagtttctttatggcagggtgaaacgcaggtcgccagcggcaccgcgccttt
cggcggtgaaattatcgatgagcgtggtggttatgccgatcgcgtcacactacgtctgaacgtcgaaaacccgaaactg
tggagcgccgaaatcccgaatctctatcgtgcggtggttgaactgcacaccgccgacggcacgctgattgaagcagaag
cctgcgatgtcggtttccgcgaggtgcggattgaaaatggtctgctgctgctgaacggcaagccgttgctgattcgagg
cgttaaccgtcacgagcatcatcctctgcatggtcaggtcatggatgagcagacgatggtgcaggatatcctgctgatg
aagcagaacaactttaacgccgtgcgctgttcgcattatccgaaccatccgctgtggtacacgctgtgcgaccgctacg
gcctgtatgtggtggatgaagccaatattgaaacccacggcatggtgccaatgaatcgtctgaccgatgatccgcgctg
gctaccggcgatgagcgaacgcgtaacgcgaatggtgcagcgcgatcgtaatcacccgagtgtgatcatctggtcgctg
gggaatgaatcaggccacggcgctaatcacgacgcgctgtatcgctggatcaaatctgtcgatccttcccgcccggtgc
agtatgaaggcggcggagccgacaccacggccaccgatattatttgcccgatgtacgcgcgcgtggatgaagaccagcc
cttcccggctgtgccgaaatggtccatcaaaaaatggctttcgctacctggagagacgcgcccgctgatcctttgcgaa
tacgcccacgcgatgggtaacagtcttggcggtttcgctaaatactggcaggcgtttcgtcagtatccccgtttacagg
gcggcttcgtctgggactgggtggatcagtcgctgattaaatatgatgaaaacggcaacccgtggtcggcttacggcgg
tgattttggcgatacgccgaacgatcgccagttctgtatgaacggtctggtctttgccgaccgcacgccgcatccagcg
ctgacggaagcaaaacaccagcagcagtttttccagttccgtttatccgggcaaaccatcgaagtgaccagcgaatacc
tgttccgtcatagcgataacgagctcctgcactggatggtggcgctggatggtaagccgctggcaagcggtgaagtgcc
tctggatgtcgctccacaaggtaaacagttgattgaactgcctgaactaccgcagccggagagcgccgggcaactctgg
ctcacagtacgcgtagtgcaaccgaacgcgaccgcatggtcagaagccgggcacatcagcgcctggcagcagtggcgtc
tggcggaaaacctcagtgtgacgctccccgccgcgtcccacgccatcccgcatctgaccaccagcgaaatggatttttg
catcgagctgggtaataagcgttggcaatttaaccgccagtcaggctttctttcacagatgtggattggcgataaaaaa
caactgctgacgccgctgcgcgatcagttcacccgtgcaccgctggataacgacattggcgtaagtgaagcgacccgca
ttgaccctaacgcctgggtcgaacgctggaaggcggcgggccattaccaggccgaagcagcgttgttgcagtgcacggc
agatacacttgctgatgcggtgctgattacgaccgctcacgcgtggcagcatcaggggaaaaccttatttatcagccgg
aaaacctaccggattgatggtagtggtcaaatggcgattaccgttgatgttgaagtggcgagcgatacaccgcatccgg
cgcggattggcctgaactgccagctggcgcaggtagcagagcgggtaaactggctcggattagggccgcaagaaaacta
tcccgaccgccttactgccgcctgttttgaccgctgggatctgccattgtcagacatgtataccccgtacgtcttcccg
agcgaaaacggtctgcgctgcgggacgcgcgaattgaattatggcccacaccagtggcgcggcgacttccagttcaaca
tcagccgctacagtcaacagcaactgatggaaaccagccatcgccatctgctgcacgcggaagaaggcacatggctgaa
tatcgacggtttccatatggggattggtggcgacgactcctggagcccgtcagtatcggcggaatttcagctgagcgcc
ggtcgctaccattaccagttggtctggtgtcaaaaataatactagagaaagaggagaaatactagatgcgtaaaggaga
agaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtgga
gagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggc
caacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgacttttt
caagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgct
gaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattc
ttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagt
taacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggc
gatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagag
accacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgc
aaacgacgaaaactacgctgctgctgtttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaaga
ctgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcct
ttctgcgtttata