##gff-version 3
##sequence-region BBa_I732014 1 947
BBa_I732014	.	promoter	1	37	.	+	0	ID=BBa_I14032;Name=BBa_I14032
BBa_I732014	.	promoter	26	31	.	+	0	ID=annotation1028344;Name=-10;Parent=BBa_I14032
BBa_I732014	.	operator	1	37	.	+	0	ID=annotation1028342;Name=P(Lac) IQ;Parent=BBa_I14032
BBa_I732014	.	promoter	3	8	.	+	0	ID=annotation1028343;Name=-35;Parent=BBa_I14032
BBa_I732014	.	engineered_region	46	947	.	+	0	ID=BBa_Q04400;Name=BBa_Q04400
BBa_I732014	.	terminator	757	836	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q04400
BBa_I732014	.	stem_loop	768	811	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I732014	.	engineered_region	757	836	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I732014	.	ribosome_entry_site	46	57	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04400
BBa_I732014	.	conserved_region	50	53	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I732014	.	terminator	845	885	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q04400
BBa_I732014	.	polyA_site	872	885	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I732014	.	stem_loop	852	871	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I732014	.	engineered_region	845	885	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I732014	.	stop_codon	878	878	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I732014	.	promoter	894	947	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_Q04400
BBa_I732014	.	promoter	913	918	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I732014	.	promoter	936	941	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I732014	.	non_covalent_binding_site	894	912	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I732014	.	engineered_region	894	947	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I732014	.	non_covalent_binding_site	919	937	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I732014	.	CDS	64	723	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_Q04400
BBa_I732014	.	CDS	67	683	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_I732014	.	tag	684	717	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_I732014	.	engineered_tag	724	748	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
>BBa_I732014
tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagaaagaggagaaatactagatgtccagattagata
aaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgccca
gaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgag
atgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaa
gttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatga
aactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtg
gggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactg
atagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggcct
tgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgcttta
gtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaa
agactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtggg
cctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac