##gff-version 3
##sequence-region BBa_I731017 1 1600
BBa_I731017	.	engineered_region	732	1600	.	+	0	ID=BBa_J06702;Name=BBa_J06702
BBa_I731017	.	ribosome_entry_site	732	743	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J06702
BBa_I731017	.	conserved_region	736	739	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I731017	.	terminator	1560	1600	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J06702
BBa_I731017	.	polyA_site	1587	1600	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I731017	.	stop_codon	1593	1593	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I731017	.	engineered_region	1560	1600	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I731017	.	stem_loop	1567	1586	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I731017	.	terminator	1472	1551	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J06702
BBa_I731017	.	stem_loop	1483	1526	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I731017	.	engineered_region	1472	1551	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I731017	.	engineered_region	750	1463	.	+	0	ID=BBa_J06504;Name=BBa_J06504;Parent=BBa_J06702
BBa_I731017	.	CDS	750	1460	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_I731017	.	sequence_alteration	1101	1101	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_I731017	.	engineered_region	1	723	.	+	0	ID=BBa_S03623;Name=BBa_S03623
BBa_I731017	.	engineered_region	63	723	.	+	0	ID=BBa_C0261;Name=BBa_C0261;Parent=BBa_S03623
BBa_I731017	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_I731017	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I731017	.	CDS	81	698	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_I731017	.	engineered_region	81	698	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_I731017	.	CDS	81	659	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_I731017	.	engineered_tag	699	723	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_I731017	.	tag	660	691	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_I731017	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_S03623
BBa_I731017	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I731017	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I731017	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I731017	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I731017	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
>BBa_I731017
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttat
caagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatg
cagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatat
gctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgtttt
gctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaac
acgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagt
tccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaat
gaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgct
agtgtagatctctactagagaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaa
ggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggc
cgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgt
cccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccc
cgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcag
gacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagacca
tgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagct
gaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctac
aacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggcc
gccactccaccggcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagt
cgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcggg
tgggcctttctgcgtttata