##gff-version 3
##sequence-region BBa_I730014 1 2244
BBa_I730014	.	CDS	167	1294	.	+	0	ID=BBa_C0012;Name=BBa_C0012
BBa_I730014	.	engineered_tag	1295	1319	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_I730014	.	engineered_region	167	1294	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_I730014	.	CDS	167	1294	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_I730014	.	tag	1256	1294	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_I730014	.	promoter	1	78	.	+	0	ID=BBa_R0083;Name=BBa_R0083
BBa_I730014	.	promoter	45	50	.	+	0	ID=annotation301224;Name=-35;Parent=BBa_R0083
BBa_I730014	.	sequence_feature	37	44	.	+	0	ID=annotation301223;Name=linker;Parent=BBa_R0083
BBa_I730014	.	point_mutation	41	41	.	+	0	ID=annotation306881;Name=A;Parent=BBa_R0083
BBa_I730014	.	promoter	68	73	.	+	0	ID=annotation301226;Name=-10;Parent=BBa_R0083
BBa_I730014	.	non_covalent_binding_site	13	30	.	+	0	ID=annotation301221;Name=C1 OmpR;Parent=BBa_R0083
BBa_I730014	.	promoter	87	140	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I730014	.	non_covalent_binding_site	112	130	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I730014	.	engineered_region	87	140	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I730014	.	non_covalent_binding_site	87	105	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I730014	.	promoter	129	134	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I730014	.	promoter	106	111	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I730014	.	engineered_region	1328	2244	.	+	0	ID=BBa_E0434;Name=BBa_E0434
BBa_I730014	.	terminator	2116	2195	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0434
BBa_I730014	.	engineered_region	2116	2195	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I730014	.	stem_loop	2127	2170	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I730014	.	terminator	2204	2244	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0434
BBa_I730014	.	engineered_region	2204	2244	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I730014	.	stop_codon	2237	2237	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I730014	.	polyA_site	2231	2244	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I730014	.	stem_loop	2211	2230	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I730014	.	engineered_region	1346	2107	.	+	0	ID=BBa_E0034;Name=BBa_E0034;Parent=BBa_E0434
BBa_I730014	.	tag	2063	2101	.	+	0	ID=annotation1245672;Name=AAV;Parent=BBa_E0034
BBa_I730014	.	stop_codon	2102	2107	.	+	0	ID=annotation1245671;Name=stop;Parent=BBa_E0034
BBa_I730014	.	ribosome_entry_site	1328	1339	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0434
BBa_I730014	.	conserved_region	1332	1335	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I730014	.	ribosome_entry_site	149	160	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I730014	.	conserved_region	153	156	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_I730014
tcccttgcatttacattttgaaacatctatagcgatctcttgagttggattattctgcatttttggggagaatggactt
actagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggaga
aatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttc
ccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattac
attcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgc
acgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtaga
acgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactat
ccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgacc
agacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtca
ccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctc
actcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaa
tgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattac
cgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccg
ccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggcc
aggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgc
ctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaac
gacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatact
agatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggcca
caagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggc
aagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccaca
tgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacgg
caactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttc
aaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagc
agaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactacca
gcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaa
gaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagc
tgtacaagaggcctgctgcaaacgacgaaaactacgctgctgctgtttaataatactagagccaggcatcaaataaaac
gaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactgg
ctcaccttcgggtgggcctttctgcgtttata