##gff-version 3 ##sequence-region BBa_I730004 1 1691 BBa_I730004 . engineered_region 775 1691 . + 0 ID=BBa_E0422;Name=BBa_E0422 BBa_I730004 . engineered_region 793 1554 . + 0 ID=BBa_E0022;Name=BBa_E0022;Parent=BBa_E0422 BBa_I730004 . tag 1511 1548 . + 0 ID=annotation2153;Name=SsrA;Parent=BBa_E0022 BBa_I730004 . CDS 793 1554 . + 0 ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022 BBa_I730004 . engineered_region 793 1554 . + 0 ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022 BBa_I730004 . stop_codon 1549 1554 . + 0 ID=annotation2154;Name=2;Parent=BBa_E0022 BBa_I730004 . point_mutation 861 861 . + 0 ID=annotation2155;Name=A;Parent=BBa_E0022 BBa_I730004 . terminator 1651 1691 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0422 BBa_I730004 . stop_codon 1684 1684 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_I730004 . polyA_site 1678 1691 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_I730004 . stem_loop 1658 1677 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_I730004 . engineered_region 1651 1691 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_I730004 . terminator 1563 1642 . + 0 ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0422 BBa_I730004 . stem_loop 1574 1617 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_I730004 . engineered_region 1563 1642 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_I730004 . ribosome_entry_site 775 786 . + 0 ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0422 BBa_I730004 . conserved_region 779 782 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_I730004 . promoter 1 54 . + 0 ID=BBa_R0011;Name=BBa_R0011 BBa_I730004 . promoter 43 48 . + 0 ID=annotation2002;Name=-10;Parent=BBa_R0011 BBa_I730004 . operator 3 19 . + 0 ID=annotation1999;Name=lac O1;Parent=BBa_R0011 BBa_I730004 . promoter 20 25 . + 0 ID=annotation2000;Name=-35;Parent=BBa_R0011 BBa_I730004 . engineered_region 1 54 . + 0 ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011 BBa_I730004 . operator 26 42 . + 0 ID=annotation2001;Name=lac O1;Parent=BBa_R0011 BBa_I730004 . CDS 82 766 . + 0 ID=BBa_C0040;Name=BBa_C0040 BBa_I730004 . tag 702 735 . + 0 ID=annotation23330;Name=SsrA;Parent=BBa_C0040 BBa_I730004 . engineered_tag 742 766 . + 0 ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040 BBa_I730004 . CDS 85 701 . + 0 ID=annotation23329;Name=tetR;Parent=BBa_C0040 BBa_I730004 . ribosome_entry_site 64 75 . + 0 ID=BBa_B0034;Name=BBa_B0034 BBa_I730004 . conserved_region 68 71 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 >BBa_I730004 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact agatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaac aacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctc gacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttt tacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcc tacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcatta tatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaa gggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagcc agccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaac gacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaagaggagaaatact agatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggcca caagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggc aagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccaca tgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacgg caactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttc aaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagc agaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactacca gcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaa gaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagc tgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaac gaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactgg ctcaccttcgggtgggcctttctgcgtttata