LOCUS       BBa_I730004             1691 bp    DNA     linear       22-Jun-2025
DEFINITION  pLacI-tetR repressor-CFP
ACCESSION   BBa_I730004
VERSION     BBa_I730004.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             85..701
                     /label=tetR
     misc_feature    702..735
                     /label=SsrA
     misc_feature    742..766
                     /label=Help:Barcodes
     CDS             82..766
                     /label=BBa_C0040
     misc_feature    779..782
                     /label=conserved
     RBS             775..786
                     /label=BBa_B0034
     CDS             793..1554
                     /label=CFP (LVA)
     misc_feature    1511..1548
                     /label=SsrA
     misc_feature    1549..1554
                     /label=2
     misc_feature    861..861
                     /label=A
     misc_feature    793..1554
                     /label=BBa_E0022
     misc_feature    793..1554
                     /label=BBa_E0022
     stem_loop       1574..1617
                     /label=stem_loop
     misc_feature    1563..1642
                     /label=BBa_B0010
     terminator      1563..1642
                     /label=BBa_B0010
     stem_loop       1658..1677
                     /label=T7 TE
     misc_feature    1684..1684
                     /label=stop
     polyA_site      1678..1691
                     /label=polya
     misc_feature    1651..1691
                     /label=BBa_B0012
     terminator      1651..1691
                     /label=BBa_B0012
     misc_feature    775..1691
                     /label=BBa_E0422
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgtccaga ttagataaaa gtaaagtgat taacagcgca
      121 ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag
      181 ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc tttgctcgac
      241 gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt agaaggggaa
      301 agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt actaagtcat
      361 cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta tgaaactctc
      421 gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc attatatgca
      481 ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga gcatcaagtc
      541 gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt acgacaagct
      601 atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct tgaattgatc
      661 atatgcggat tagaaaaaca acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac
      721 tacgctttag tagcttaata acactgatag tgctagtgta gatcactact agagaaagag
      781 gagaaatact agatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      841 gtcgagctgg acggcgacgt gaacggccac aagttcagcg tgtccggcga gggcgagggc
      901 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      961 ccctggccca ccctcgtgac caccctgacc tggggcgtgc agtgcttcag ccgctacccc
     1021 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     1081 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     1141 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1201 atcctggggc acaagctgga gtacaactac atcagccaca acgtctatat caccgccgac
     1261 aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcagc
     1321 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1381 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1441 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1501 ctgtacaaga ggcctgctgc aaacgacgaa aactacgctt tagtagctta ataatactag
     1561 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     1621 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
     1681 ctgcgtttat a
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