##gff-version 3
##sequence-region BBa_I728655 1 3365
BBa_I728655	.	engineered_region	2488	3365	.	+	0	ID=BBa_E0840;Name=BBa_E0840
BBa_I728655	.	ribosome_entry_site	2488	2502	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_E0840
BBa_I728655	.	engineered_region	2488	2502	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_I728655	.	ribosome_entry_site	2488	2502	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_I728655	.	ribosome_entry_site	2495	2499	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_I728655	.	CDS	2509	3228	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0840
BBa_I728655	.	CDS	2509	3228	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_I728655	.	terminator	3237	3316	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0840
BBa_I728655	.	stem_loop	3248	3291	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I728655	.	engineered_region	3237	3316	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I728655	.	terminator	3325	3365	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0840
BBa_I728655	.	engineered_region	3325	3365	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I728655	.	stop_codon	3358	3358	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I728655	.	polyA_site	3352	3365	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I728655	.	stem_loop	3332	3351	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I728655	.	engineered_region	1493	2479	.	+	0	ID=BBa_Q04510;Name=BBa_Q04510
BBa_I728655	.	terminator	2382	2422	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q04510
BBa_I728655	.	engineered_region	2382	2422	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I728655	.	stop_codon	2415	2415	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I728655	.	polyA_site	2409	2422	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I728655	.	stem_loop	2389	2408	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I728655	.	promoter	2431	2479	.	+	0	ID=BBa_R0051;Name=BBa_R0051;Parent=BBa_Q04510
BBa_I728655	.	engineered_region	2431	2479	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_I728655	.	promoter	2445	2450	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_I728655	.	promoter	2468	2473	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_I728655	.	non_covalent_binding_site	2431	2447	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_I728655	.	non_covalent_binding_site	2455	2471	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_I728655	.	terminator	2294	2373	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q04510
BBa_I728655	.	stem_loop	2305	2348	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I728655	.	engineered_region	2294	2373	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I728655	.	ribosome_entry_site	1493	1504	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04510
BBa_I728655	.	conserved_region	1497	1500	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I728655	.	CDS	1511	2260	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_Q04510
BBa_I728655	.	tag	2222	2254	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_I728655	.	engineered_tag	2261	2285	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_I728655	.	CDS	1514	2221	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_I728655	.	engineered_region	642	1484	.	+	0	ID=BBa_J04650;Name=BBa_J04650
BBa_I728655	.	terminator	1444	1484	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J04650
BBa_I728655	.	engineered_region	1444	1484	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I728655	.	stop_codon	1477	1477	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I728655	.	polyA_site	1471	1484	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I728655	.	stem_loop	1451	1470	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I728655	.	terminator	1356	1435	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J04650
BBa_I728655	.	stem_loop	1367	1410	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I728655	.	engineered_region	1356	1435	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I728655	.	CDS	642	1322	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J04650
BBa_I728655	.	CDS	642	1316	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_I728655	.	engineered_tag	1323	1347	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_I728655	.	promoter	1	635	.	+	0	ID=BBa_I728456;Name=BBa_I728456
BBa_I728655	.	CDS	54	479	.	+	0	ID=annotation1975680;Name=MerR;Parent=BBa_I728456
BBa_I728655	.	CDS	561	628	.	+	0	ID=annotation1975682;Name=MerT (first part);Parent=BBa_I728456
BBa_I728655	.	promoter	480	560	.	+	0	ID=annotation1975681;Name=-35 and -10 hexamers;Parent=BBa_I728456
>BBa_I728655
ctctttgaccaggctttctaaggggtcgtctcagaaaacggaaaataaagcacgctaaggcatagccgaacctgccaag
cttgctccaccctgtagtgacgcgatcagcgggcaggaaacgttcccccttcgcgcatggcaggcgcacaccaactcag
acagcacggcctccatgcgcgccaggtcagccattttctcgcgcacgtccttgagcttgtgctcggccagactgctggc
ttcctcgcaatgggtgccatcctccagccgcagcagctcggcgatctcatccaggctgaagcccagccgctgggctgat
ttcacgaagcgcacccgcgttacatccgcctcgccatagcggcggatgctgccatagggcttgtcaggctccagcaaca
agcccttgcgctgatagaaacggatggtctccacattgaccccggccgccttggcgaaaacgccaatggtcaggttctc
caaattgttttccatatcgcttgactccgtacatgagtacggaagtaaggttacgctatccaatttcaattcgaaagga
caagcgcatgtctgaaccaaaaaccgggcgcggcgcgctcttcactggagggcttgccgctatccttgcttctgcttgt
tagtactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacg
gtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaa
aggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccg
gctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtg
gtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaactt
cccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggt
gctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctaca
tggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagacta
caccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtaga
tcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtc
ggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaaagaggag
aaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatg
aaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgc
tttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaa
tttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatg
agtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagag
atgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaaca
ggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgca
tagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaa
cccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagag
acgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactag
agccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgct
ctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaacaccgtgcgtgttgac
tattttacctctggcggtgataatggttgctactagagattaaagaggagaaatactagatgcgtaaaggagaagaact
tttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggt
gaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacac
ttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagag
tgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtc
aagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggac
acaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaactt
caaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggc
cctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccaca
tggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagcca
ggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcta
ctagagtcacactggctcaccttcgggtgggcctttctgcgtttata