##gff-version 3
##sequence-region BBa_I728500 1 654
BBa_I728500	.	sequence_feature	175	192	.	+	0	ID=annotation1940504;Name=Linker
BBa_I728500	.	CDS	193	477	.	+	0	ID=annotation1940505;Name=S54-F148 of OmpX
BBa_I728500	.	CDS	91	129	.	+	0	ID=annotation1940503;Name=Polystyrene Sticky Peptide
BBa_I728500	.	sequence_feature	70	84	.	+	0	ID=annotation1940502;Name=SfiI Restriction Site
BBa_I728500	.	sequence_feature	130	135	.	+	0	ID=annotation1940511;Name=SalI Restriction Site
BBa_I728500	.	stop_codon	649	654	.	+	0	ID=annotation1940508;Name=Double stop codon (TGATAA)
BBa_I728500	.	start_codon	1	3	.	+	0	ID=annotation1940500;Name=Start codon
BBa_I728500	.	sequence_feature	478	489	.	+	0	ID=annotation1940506;Name=Linker
BBa_I728500	.	CDS	136	141	.	+	0	ID=annotation1940512;Name=Trypsin Cleavage Site
BBa_I728500	.	CDS	1	654	.	+	0	ID=annotation1975607;Name=Coding sequence
BBa_I728500	.	tag	142	174	.	+	0	ID=annotation1940510;Name=T7 Antibody Tag
BBa_I728500	.	CDS	490	648	.	+	0	ID=annotation1940507;Name=A1-S53 of OmpX
BBa_I728500	.	sequence_feature	85	90	.	+	0	ID=annotation1940509;Name=BglII Cut Site
BBa_I728500	.	CDS	1	69	.	+	0	ID=annotation1940501;Name=Native Starting Signal
>BBa_I728500
atgaaaaaaattgcgtgcctgagcgcgctggccgcggttctggcctttaccgcgggcaccagcgttgcgggccagtctg
gccaaagatcttttttcagctttttttttccggcgagcgcgtggggtagcgtcgacaaacgtatggcgagcatgaccgg
cggtcagcagatgggtggtggccagagcggccagagcggtgattataacaaaaaccagtattatggcattaccgcgggt
ccggcgtatcgtattaacgattgggcgagcatttatggcgtggtgggcgtgggctatggcaaatttcagaccaccgaat
atccgacctataaacacgataccagcgattatggctttagctatggcgcgggtctgcaatttaatccgatggaaaacgt
ggcgctggattttagctatgaacagagccgtattcgtagcgtggatgtgggcacctggattgcgggcgtgggttatcgt
tttggcggcagcggtgcgaccagcaccgtgaccggcggttatgcgcagagcgatgcgcagggccagatgaacaaaatgg
gcggcttcaacctgaaatatcgctatgaagaagataacagcccgctgggcgtgattggcagctttacctataccgaaaa
aagccgtaccgcgagctgataa