##gff-version 3
##sequence-region BBa_I724007 1 2574
BBa_I724007	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I724007	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I724007	.	ribosome_entry_site	1639	1650	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I724007	.	conserved_region	1643	1646	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I724007	.	terminator	1547	1587	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I724007	.	engineered_region	1547	1587	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I724007	.	stop_codon	1580	1580	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I724007	.	stem_loop	1554	1573	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I724007	.	polyA_site	1574	1587	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I724007	.	engineered_region	731	1450	.	+	0	ID=BBa_J04051;Name=BBa_J04051
BBa_I724007	.	CDS	731	1405	.	+	0	ID=annotation1507347;Name=mrfp1;Parent=BBa_J04051
BBa_I724007	.	start_codon	731	733	.	+	0	ID=annotation1508160;Name=Start;Parent=BBa_J04051
BBa_I724007	.	tag	1406	1444	.	+	0	ID=annotation1507968;Name=LVA;Parent=BBa_J04051
BBa_I724007	.	stop_codon	1445	1450	.	+	0	ID=annotation1507863;Name=Stop;Parent=BBa_J04051
BBa_I724007	.	terminator	2446	2525	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I724007	.	engineered_region	2446	2525	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I724007	.	stem_loop	2457	2500	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I724007	.	terminator	2534	2574	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I724007	.	engineered_region	2534	2574	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I724007	.	stop_codon	2567	2567	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I724007	.	stem_loop	2541	2560	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I724007	.	polyA_site	2561	2574	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I724007	.	promoter	1596	1630	.	+	0	ID=BBa_J23119;Name=BBa_J23119
BBa_I724007	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_I724007	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_I724007	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_I724007	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_I724007	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_I724007	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_I724007	.	CDS	1657	2412	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_I724007	.	point_mutation	2148	2148	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_I724007	.	CDS	1657	2406	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_I724007	.	engineered_region	1657	2412	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_I724007	.	engineered_tag	2413	2437	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_I724007	.	point_mutation	1830	1830	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_I724007	.	engineered_region	1657	1658	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_I724007	.	CDS	82	699	.	+	0	ID=BBa_C0061;Name=BBa_C0061
BBa_I724007	.	CDS	82	660	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_I724007	.	engineered_tag	700	724	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_I724007	.	engineered_region	82	699	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_I724007	.	tag	661	692	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_I724007	.	terminator	1459	1538	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I724007	.	engineered_region	1459	1538	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I724007	.	stem_loop	1470	1513	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_I724007
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgtta
tcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaat
gcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattata
tgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttt
tgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaa
cacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaag
ttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaa
tgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgc
tagtgtagatctctactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggt
tccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactga
aagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgt
taaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttc
gaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtg
gtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtaccc
ggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaa
accacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccaca
acgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgctaggcctgctgcaaacga
cgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggc
ctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgc
gtttatatactagagttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatg
aaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaat
gcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatc
tgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcct
atagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctc
caaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggctt
cggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacatacca
ttaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaa
gagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcg
tactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaatt
ttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccag
gcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctac
tagagtcacactggctcaccttcgggtgggcctttctgcgtttata