##gff-version 3
##sequence-region BBa_I724004 1 2884
BBa_I724004	.	terminator	2756	2835	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I724004	.	stem_loop	2767	2810	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I724004	.	engineered_region	2756	2835	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I724004	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I724004	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I724004	.	promoter	1906	1959	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I724004	.	non_covalent_binding_site	1906	1924	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I724004	.	engineered_region	1906	1959	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I724004	.	promoter	1925	1930	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I724004	.	promoter	1948	1953	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I724004	.	non_covalent_binding_site	1931	1949	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I724004	.	terminator	864	943	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I724004	.	stem_loop	875	918	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I724004	.	engineered_region	864	943	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I724004	.	terminator	1769	1848	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I724004	.	stem_loop	1780	1823	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I724004	.	engineered_region	1769	1848	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I724004	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I724004	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I724004	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I724004	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I724004	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I724004	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I724004	.	CDS	81	830	.	+	0	ID=BBa_C0051;Name=BBa_C0051
BBa_I724004	.	tag	792	824	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_I724004	.	engineered_tag	831	855	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_I724004	.	CDS	84	791	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_I724004	.	engineered_region	1986	2747	.	+	0	ID=BBa_E0034;Name=BBa_E0034
BBa_I724004	.	stop_codon	2742	2747	.	+	0	ID=annotation1245671;Name=stop;Parent=BBa_E0034
BBa_I724004	.	tag	2703	2741	.	+	0	ID=annotation1245672;Name=AAV;Parent=BBa_E0034
BBa_I724004	.	CDS	1076	1735	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_I724004	.	engineered_tag	1736	1760	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_I724004	.	CDS	1079	1695	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_I724004	.	tag	1696	1729	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_I724004	.	terminator	1857	1897	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I724004	.	stem_loop	1864	1883	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I724004	.	stop_codon	1890	1890	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I724004	.	engineered_region	1857	1897	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I724004	.	polyA_site	1884	1897	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I724004	.	ribosome_entry_site	1058	1069	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I724004	.	conserved_region	1062	1065	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I724004	.	promoter	1001	1049	.	+	0	ID=BBa_R0051;Name=BBa_R0051
BBa_I724004	.	promoter	1015	1020	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_I724004	.	non_covalent_binding_site	1025	1041	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_I724004	.	non_covalent_binding_site	1001	1017	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_I724004	.	engineered_region	1001	1049	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_I724004	.	promoter	1038	1043	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_I724004	.	terminator	952	992	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I724004	.	stem_loop	959	978	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I724004	.	stop_codon	985	985	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I724004	.	engineered_region	952	992	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I724004	.	polyA_site	979	992	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I724004	.	terminator	2844	2884	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I724004	.	stem_loop	2851	2870	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I724004	.	stop_codon	2877	2877	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I724004	.	engineered_region	2844	2884	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I724004	.	polyA_site	2871	2884	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I724004	.	ribosome_entry_site	1968	1979	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I724004	.	conserved_region	1972	1975	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_I724004
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaag
aaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttattta
atggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagccc
ttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccct
gttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaa
gcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaa
gccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccaga
cttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagt
acccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttgg
cgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggc
atcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctacta
gagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaacaccgtgcgtgttgactattttac
ctctggcggtgataatggttgctactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgatta
acagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtaga
gcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcac
catactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctt
tactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatca
attagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttacttta
ggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccat
tattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatg
cggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacac
tgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcg
ttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttat
atactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagagga
gaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgta
aacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgca
ccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccc
cgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaag
gacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggca
tcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggc
cgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgac
cactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccc
tgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcat
ggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctgctgctgtttaataatactagagccaggcatca
aataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagt
cacactggctcaccttcgggtgggcctttctgcgtttata