##gff-version 3
##sequence-region BBa_I724003 1 2476
BBa_I724003	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_I724003	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_I724003	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_I724003	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_I724003	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_I724003	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_I724003	.	terminator	2397	2476	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I724003	.	engineered_region	2397	2476	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I724003	.	stem_loop	2408	2451	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I724003	.	CDS	1608	2363	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_I724003	.	point_mutation	2099	2099	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_I724003	.	CDS	1608	2357	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_I724003	.	engineered_region	1608	2363	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_I724003	.	engineered_tag	2364	2388	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_I724003	.	point_mutation	1781	1781	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_I724003	.	engineered_region	1608	1609	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_I724003	.	engineered_region	731	1450	.	+	0	ID=BBa_J04051;Name=BBa_J04051
BBa_I724003	.	CDS	731	1405	.	+	0	ID=annotation1507347;Name=mrfp1;Parent=BBa_J04051
BBa_I724003	.	start_codon	731	733	.	+	0	ID=annotation1508160;Name=Start;Parent=BBa_J04051
BBa_I724003	.	tag	1406	1444	.	+	0	ID=annotation1507968;Name=LVA;Parent=BBa_J04051
BBa_I724003	.	stop_codon	1445	1450	.	+	0	ID=annotation1507863;Name=Stop;Parent=BBa_J04051
BBa_I724003	.	promoter	1547	1581	.	+	0	ID=BBa_J23119;Name=BBa_J23119
BBa_I724003	.	CDS	82	699	.	+	0	ID=BBa_C0061;Name=BBa_C0061
BBa_I724003	.	CDS	82	660	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_I724003	.	engineered_tag	700	724	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_I724003	.	engineered_region	82	699	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_I724003	.	tag	661	692	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_I724003	.	terminator	1459	1538	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I724003	.	engineered_region	1459	1538	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I724003	.	stem_loop	1470	1513	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I724003	.	ribosome_entry_site	1590	1601	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I724003	.	conserved_region	1594	1597	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I724003	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I724003	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_I724003
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgtta
tcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaat
gcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattata
tgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttt
tgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaa
cacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaag
ttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaa
tgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgc
tagtgtagatctctactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggt
tccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactga
aagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgt
taaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttc
gaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtg
gtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtaccc
ggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaa
accacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccaca
acgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgctaggcctgctgcaaacga
cgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggc
ctttcgttttatctgttgtttgtcggtgaacgctctctactagagttgacagctagctcagtcctaggtataatgctag
ctactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaag
cttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgc
gatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattat
gatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatat
ttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttag
tttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagt
ttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaa
ataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttggga
tatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaaca
aaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgat
agtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttt
atctgttgtttgtcggtgaacgctctc