##gff-version 3
##sequence-region BBa_I724001 1 3062
BBa_I724001	.	engineered_region	2168	3062	.	+	0	ID=BBa_I724000;Name=BBa_I724000
BBa_I724001	.	promoter	2168	2222	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_I724000
BBa_I724001	.	promoter	2187	2192	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_I724001	.	promoter	2209	2214	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_I724001	.	start_codon	2220	2220	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_I724001	.	engineered_region	2168	2222	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_I724001	.	non_covalent_binding_site	2168	2187	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_I724001	.	ribosome_entry_site	2231	2242	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I724000
BBa_I724001	.	conserved_region	2235	2238	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I724001	.	CDS	2249	3037	.	+	0	ID=BBa_C0060;Name=BBa_C0060;Parent=BBa_I724000
BBa_I724001	.	stop_codon	3032	3037	.	+	0	ID=annotation1755;Name=2;Parent=BBa_C0060
BBa_I724001	.	engineered_region	2249	3037	.	+	0	ID=annotation7037;Name=BBa_C0060;Parent=BBa_C0060
BBa_I724001	.	CDS	2249	2998	.	+	0	ID=annotation1757;Name=aiiA;Parent=BBa_C0060
BBa_I724001	.	start_codon	2249	2251	.	+	0	ID=annotation1754;Name=start;Parent=BBa_C0060
BBa_I724001	.	tag	2999	3031	.	+	0	ID=annotation1756;Name=LVA;Parent=BBa_C0060
BBa_I724001	.	engineered_tag	3038	3062	.	+	0	ID=annotation2213987;Name=Help:Barcodes;Parent=BBa_C0060
BBa_I724001	.	engineered_region	1	2159	.	+	0	ID=BBa_J40001;Name=BBa_J40001
BBa_I724001	.	promoter	1	55	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_J40001
BBa_I724001	.	promoter	20	25	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_I724001	.	promoter	42	47	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_I724001	.	start_codon	53	53	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_I724001	.	engineered_region	1	55	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_I724001	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_I724001	.	engineered_region	1243	2159	.	+	0	ID=BBa_E0422;Name=BBa_E0422;Parent=BBa_J40001
BBa_I724001	.	ribosome_entry_site	1243	1254	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0422
BBa_I724001	.	conserved_region	1247	1250	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I724001	.	terminator	2119	2159	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0422
BBa_I724001	.	engineered_region	2119	2159	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I724001	.	stop_codon	2152	2152	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I724001	.	polyA_site	2146	2159	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I724001	.	stem_loop	2126	2145	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I724001	.	terminator	2031	2110	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0422
BBa_I724001	.	engineered_region	2031	2110	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I724001	.	stem_loop	2042	2085	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I724001	.	engineered_region	1261	2022	.	+	0	ID=BBa_E0022;Name=BBa_E0022;Parent=BBa_E0422
BBa_I724001	.	stop_codon	2017	2022	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_I724001	.	CDS	1261	2022	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_I724001	.	tag	1979	2016	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
BBa_I724001	.	engineered_region	1261	2022	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
BBa_I724001	.	point_mutation	1329	1329	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_I724001	.	engineered_region	64	1234	.	+	0	ID=BBa_S0100;Name=BBa_S0100;Parent=BBa_J40001
BBa_I724001	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S0100
BBa_I724001	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I724001	.	CDS	82	1209	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_S0100
BBa_I724001	.	engineered_region	82	1209	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_I724001	.	tag	1171	1209	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_I724001	.	engineered_tag	1210	1234	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_I724001	.	CDS	82	1209	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
>BBa_I724001
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatact
agatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgt
ggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattccc
aaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgc
cgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaag
cggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctg
gatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacac
ccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagca
aatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgc
aatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctga
atgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtc
cgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgtta
accaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcgg
tgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctcc
ccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaa
aactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatgg
tgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagtt
cagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctg
cccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagc
agcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaacta
caagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggag
gacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaaga
acggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaa
cacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagacccc
aacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtaca
agaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaagg
ctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacc
ttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttata
gtcgaataaatactagagaaagaggagaaatactagatgacagtaaagaagctttatttcgtcccagcaggtcgttgta
tgttggatcattcgtctgttaatagtacattaacaccaggagaattattagacttaccggtttggtgttatcttttgga
gactgaagaaggacctattttagtagatacaggtatgccagaaagtgcagttaataatgaaggtctttttaacggtaca
tttgtcgaagggcaggttttaccgaaaatgactgaagaagatagaatcgtgaatattttaaaacgggttggttatgagc
cggaagaccttctttatattattagttctcacttgcattttgatcatgcaggaggaaatggcgcttttataaatacacc
aatcattgtacagcgtgctgaatatgaggcggcgcagcatagcgaagaatatttgaaagaatgtatattgccgaattta
aactacaaaatcattgaaggtgattatgaagtcgtaccaggagttcaattattgcatacaccaggccatactccagggc
atcaatcgctattaattgagacagaaaaatccggtcctgtattattaacgattgatgcatcgtatacgaaagagaattt
tgaaaatgaagtgccatttgcgggatttgattcagaattagctttatcttcaattaaacgtttaaaagaagtggtgatg
aaagagaagccgattgttttctttggacatgatatagagcaggaaaggggatgtaaagtgttccctgaatatatagctg
caaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc