##gff-version 3
##sequence-region BBa_I722012 1 2722
BBa_I722012	.	ribosome_entry_site	2711	2722	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I722012	.	conserved_region	2715	2718	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I722012	.	engineered_region	1	2702	.	+	0	ID=BBa_I722011;Name=BBa_I722011
BBa_I722012	.	engineered_region	1	1633	.	+	0	ID=BBa_I0405;Name=BBa_I0405;Parent=BBa_I722011
BBa_I722012	.	ribosome_entry_site	756	767	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0405
BBa_I722012	.	conserved_region	760	763	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I722012	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0405
BBa_I722012	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I722012	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_I0405
BBa_I722012	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I722012	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I722012	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I722012	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I722012	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I722012	.	CDS	81	722	.	+	0	ID=BBa_C0070;Name=BBa_C0070;Parent=BBa_I0405
BBa_I722012	.	engineered_tag	723	747	.	+	0	ID=annotation2213996;Name=Help:Barcodes;Parent=BBa_C0070
BBa_I722012	.	CDS	81	683	.	+	0	ID=annotation301203;Name=rhlL;Parent=BBa_C0070
BBa_I722012	.	tag	684	716	.	+	0	ID=annotation306820;Name=LVA;Parent=BBa_C0070
BBa_I722012	.	terminator	1505	1584	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0405
BBa_I722012	.	stem_loop	1516	1559	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I722012	.	engineered_region	1505	1584	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I722012	.	terminator	1593	1633	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0405
BBa_I722012	.	stem_loop	1600	1619	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I722012	.	polyA_site	1620	1633	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I722012	.	stop_codon	1626	1626	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I722012	.	engineered_region	1593	1633	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I722012	.	CDS	774	1496	.	+	0	ID=BBa_E0020;Name=BBa_E0020;Parent=BBa_I0405
BBa_I722012	.	engineered_region	1642	2702	.	+	0	ID=BBa_F2620;Name=BBa_F2620;Parent=BBa_I722011
BBa_I722012	.	CDS	1722	2477	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_F2620
BBa_I722012	.	engineered_tag	2478	2502	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_I722012	.	engineered_region	1722	1723	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_I722012	.	point_mutation	1895	1895	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_I722012	.	engineered_region	1722	2477	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_I722012	.	point_mutation	2213	2213	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_I722012	.	CDS	1722	2471	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_I722012	.	ribosome_entry_site	1704	1715	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F2620
BBa_I722012	.	conserved_region	1708	1711	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I722012	.	promoter	2648	2702	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_F2620
BBa_I722012	.	engineered_region	2648	2702	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_I722012	.	promoter	2667	2672	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_I722012	.	start_codon	2700	2700	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_I722012	.	promoter	2689	2694	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_I722012	.	non_covalent_binding_site	2648	2667	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_I722012	.	promoter	1642	1695	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_F2620
BBa_I722012	.	engineered_region	1642	1695	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I722012	.	promoter	1661	1666	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I722012	.	non_covalent_binding_site	1667	1685	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I722012	.	promoter	1684	1689	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I722012	.	non_covalent_binding_site	1642	1660	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I722012	.	terminator	2511	2639	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_F2620
BBa_I722012	.	terminator	2599	2639	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I722012	.	stem_loop	2606	2625	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I722012	.	polyA_site	2626	2639	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I722012	.	stop_codon	2632	2632	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I722012	.	engineered_region	2599	2639	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I722012	.	terminator	2511	2590	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I722012	.	stem_loop	2522	2565	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I722012	.	engineered_region	2511	2590	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_I722012
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgatcgaactgctgtccgaatccctggaaggtctgtccgctgctatgatcgctgaactgggtcgttaccgtcaccag
gttttcatcgaaaaactgggttgggacgttgtttccacctcccgtgttcgtgaccaggagttcgaccagttcgaccacc
cgcagacccgttacatcgttgctatgtcccgtcagggtatctgcggttgcgctcgtctgctgccgaccaccgacgctta
cctgctgaaagacgttttcgcttacctgtgctccgaaaccccgccgtccgacccgtccgtttgggaactgtcccgttac
gctgcttccgctgctgacgacccgcagctggctatgaaaatcttctggtcctccctccagtgcgcttggtacctgggtg
cttcctccgttgttgctgttaccaccaccgctatggaacgttacttcgttcgtaacggtgttatcctccagcgtctggg
tccgccgcagaaagttaaaggtgaaaccctggttgctatctccttcccggcttaccaggaacgtggtctggaaatgctg
ctgcgttaccacccggaatggctccagggtgttccgctgtccatggctgttgctgcaaacgacgaaaactacgctttag
tagcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatggtgagcaagggcga
ggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggc
gagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggc
ccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttctt
caagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgcc
gaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcc
tggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggc
caacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggc
gacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcg
atcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatacta
gagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgc
tctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagag
attgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaaaaacataaatgc
cgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatg
actaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcc
tagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattc
taactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaa
gaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagtt
ttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttc
tctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgt
ttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttcc
atttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaat
tgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaac
gaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactgg
ctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggttt
gttatagtcgaataaatactagagaaagaggagaaa