##gff-version 3 ##sequence-region BBa_I721008 1 3012 BBa_I721008 . ribosome_entry_site 1922 1933 . + 0 ID=BBa_B0034;Name=BBa_B0034 BBa_I721008 . conserved_region 1926 1929 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_I721008 . ribosome_entry_site 63 74 . + 0 ID=BBa_B0034;Name=BBa_B0034 BBa_I721008 . conserved_region 67 70 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_I721008 . terminator 2884 2963 . + 0 ID=BBa_B0010;Name=BBa_B0010 BBa_I721008 . stem_loop 2895 2938 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_I721008 . engineered_region 2884 2963 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_I721008 . terminator 1742 1821 . + 0 ID=BBa_B0010;Name=BBa_B0010 BBa_I721008 . stem_loop 1753 1796 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_I721008 . engineered_region 1742 1821 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_I721008 . promoter 1 54 . + 0 ID=BBa_R0040;Name=BBa_R0040 BBa_I721008 . promoter 20 25 . + 0 ID=annotation1986785;Name=-35;Parent=BBa_R0040 BBa_I721008 . promoter 43 48 . + 0 ID=annotation1986787;Name=-10;Parent=BBa_R0040 BBa_I721008 . non_covalent_binding_site 1 19 . + 0 ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040 BBa_I721008 . engineered_region 1 54 . + 0 ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040 BBa_I721008 . non_covalent_binding_site 26 44 . + 0 ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040 BBa_I721008 . terminator 1830 1870 . + 0 ID=BBa_B0012;Name=BBa_B0012 BBa_I721008 . polyA_site 1857 1870 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_I721008 . stem_loop 1837 1856 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_I721008 . engineered_region 1830 1870 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_I721008 . stop_codon 1863 1863 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_I721008 . engineered_region 1940 2875 . + 0 ID=BBa_J52008;Name=BBa_J52008 BBa_I721008 . engineered_region 1940 2875 . + 0 ID=annotation1902833;Name=Rluc;Parent=BBa_J52008 BBa_I721008 . CDS 1940 2875 . + 0 ID=annotation1902845;Name=Rluc;Parent=BBa_J52008 BBa_I721008 . terminator 2972 3012 . + 0 ID=BBa_B0012;Name=BBa_B0012 BBa_I721008 . polyA_site 2999 3012 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_I721008 . stem_loop 2979 2998 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_I721008 . engineered_region 2972 3012 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_I721008 . stop_codon 3005 3005 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_I721008 . CDS 81 1733 . + 0 ID=BBa_I712019;Name=BBa_I712019 BBa_I721008 . promoter 1879 1913 . + 0 ID=BBa_J23100;Name=BBa_J23100 >BBa_I721008 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta gatggaagacgccaaaaacataaagaaaggcccggcgccattctatccgctggaagatggaaccgctggagagcaactg cataaggctatgaagagatacgccctggttcctggaacaattgcttttacagatgcacatatcgaggtggacatcactt acgctgagtacttcgaaatgtccgttcggttggcagaagctatgaaacgatatgggctgaatacaaatcacagaatcgt cgtatgcagtgaaaactctcttcaattctttatgccggtgttgggcgcgttatttatcggagttgcagttgcgcccgcg aacgacatttataatgaacgtgaattgctcaacagtatgggcatttcgcagcctaccgtggtgttcgtttccaaaaagg ggttgcaaaaaattttgaacgtgcaaaaaaagctcccaatcatccaaaaaattattatcatggattctaaaacggatta ccagggatttcagtcgatgtacacgttcgtcacatctcatctacctcccggttttaatgaatacgattttgtgccagag tccttcgatagggacaagacaattgcactgatcatgaactcctctggatctactggtctgcctaaaggtgtcgctctgc ctcatagaactgcctgcgtgagattctcgcatgccagagatcctatttttggcaatcaaatcattccggatactgcgat tttaagtgttgttccattccatcacggttttggaatgtttactacactcggatatttgatatgtggatttcgagtcgtc ttaatgtatagatttgaagaagagctgtttctgaggagccttcaggattacaagattcaaagtgcgctgctggtgccaa ccctattctccttcttcgccaaaagcactctgattgacaaatacgatttatctaatttacacgaaattgcttctggtgg cgctcccctctctaaggaagtcggggaagcggttgccaagaggttccatctgccaggtatcaggcaaggatatgggctc actgagactacatcagctattctgattacacccgagggggatgataaaccgggcgcggtcggtaaagttgttccatttt ttgaagcgaaggttgtggatctggataccgggaaaacgctgggcgttaatcaaagaggcgaactgtgtgtgagaggtcc tatgattatgtccggttatgtaaacaatccggaagcgaccaacgccttgattgacaaggatggatggctacattctgga gacatagcttactgggacgaagacgaacacttcttcatcgttgaccgcctgaagtctctgattaagtacaaaggctatc aggtggctcccgctgaattggaatccatcttgctccaacaccccaacatcttcgacgcaggtgtcgcaggtcttcccga cgatgacgccggtgaacttcccgccgccgttgttgttttggagcacggaaagacgatgacggaaaaagagatcgtggat tacgtcgccagtcaagtaacaaccgcgaaaaagttgcgcggaggagttgtgtttgtggacgaagtaccgaaaggtctta ccggaaaactcgacgcaagaaaaatcagagagatcctcataaaggccaagaagggcggaaagatcgccgtgtaatacta gagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgc tctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacggctagctcagtc ctaggtacagtgctagctactagagaaagaggagaaatactagatggcttccaaggtgtacgaccccgagcaacgcaaa cgcatgatcactgggcctcagtggtgggctcgctgcaagcaaatgaacgtgctggactccttcatcaactactatgatt ccgagaagcacgccgagaacgccgtgatttttctgcatggtaacgctgcctccagctacctgtggaggcacgtcgtgcc tcacatcgagcccgtggctagatgcatcatccctgatctgatcggaatgggtaagtccggcaagagcgggaatggctca tatcgcctcctggatcactacaagtacctcaccgcttggttcgagctgctgaaccttccaaagaaaatcatctttgtgg gccacgactggggggcttgtctggcctttcactactcctacgagcaccaagacaagatcaaggccatcgtccatgctga gagtgtcgtggacgtgatcgagtcctgggacgagtggcctgacatcgaggaggatatcgccctgatcaagagcgaagag ggcgagaaaatggtgcttgagaataacttcttcgtcgagaccatgctcccaagcaagatcatgcggaaactggagcctg aggagttcgctgcctacctggagccattcaaggagaagggcgaggttagacggcctaccctctcctggcctcgcgagat ccctctcgttaagggaggcaagcccgacgtcgtccagattgtccgcaactacaacgcctaccttcgggccagcgacgat ctgcctaagatgttcatcgagtccgaccctgggttcttttccaacgctattgtcgagggagctaagaagttccctaaca ccgagttcgtgaaggtgaagggcctccacttcagccaggaggacgctccagatgaaatgggtaagtacatcaagagctt cgtggagcgcgtgctgaagaacgagcagtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactg ggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttc tgcgtttata