##gff-version 3
##sequence-region BBa_I715044 1 2132
BBa_I715044	.	engineered_region	554	803	.	+	0	ID=BBa_I715020;Name=BBa_I715020
BBa_I715044	.	sequence_feature	554	554	.	+	0	ID=annotation1935360;Name=extra base to maintain reading frame;Parent=BBa_I715020
BBa_I715044	.	CDS	554	797	.	+	0	ID=annotation1935361;Name=Coding Carboxyl half of GFP (aka GFP2);Parent=BBa_I715020
BBa_I715044	.	stop_codon	798	803	.	+	0	ID=annotation1935362;Name=Stop Codons (two in tandem);Parent=BBa_I715020
BBa_I715044	.	CDS	50	511	.	+	0	ID=BBa_I715022;Name=BBa_I715022
BBa_I715044	.	start_codon	50	52	.	+	0	ID=annotation1935380;Name=start;Parent=BBa_I715022
BBa_I715044	.	CDS	50	511	.	+	0	ID=annotation1935381;Name=Coding sequence;Parent=BBa_I715022
BBa_I715044	.	promoter	1	23	.	+	0	ID=BBa_R0085;Name=BBa_R0085
BBa_I715044	.	sequence_feature	12	23	.	+	0	ID=annotation1721522;Name=Initiation Region;Parent=BBa_R0085
BBa_I715044	.	non_covalent_binding_site	1	11	.	+	0	ID=annotation1721521;Name=Polymerase Binding Region;Parent=BBa_R0085
BBa_I715044	.	sequence_feature	18	18	.	+	0	ID=annotation1721520;Name=Transcription Start Site;Parent=BBa_R0085
BBa_I715044	.	ribosome_entry_site	1211	1222	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I715044	.	conserved_region	1215	1218	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I715044	.	terminator	812	891	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I715044	.	engineered_region	812	891	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I715044	.	stem_loop	823	866	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I715044	.	terminator	1970	2049	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I715044	.	engineered_region	1970	2049	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I715044	.	stem_loop	1981	2024	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I715044	.	ribosome_entry_site	32	43	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I715044	.	conserved_region	36	39	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I715044	.	sequence_feature	520	545	.	+	0	ID=BBa_J44000;Name=BBa_J44000
BBa_I715044	.	sequence_feature	949	974	.	+	0	ID=BBa_J44000;Name=BBa_J44000
BBa_I715044	.	sequence_feature	2107	2132	.	+	0	ID=BBa_J44000;Name=BBa_J44000
BBa_I715044	.	terminator	900	940	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I715044	.	engineered_region	900	940	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I715044	.	stop_codon	933	933	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I715044	.	stem_loop	907	926	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I715044	.	polyA_site	927	940	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I715044	.	engineered_region	1229	1699	.	+	0	ID=BBa_I715019;Name=BBa_I715019
BBa_I715044	.	start_codon	1229	1231	.	+	0	ID=annotation1935356;Name=Start Codon;Parent=BBa_I715019
BBa_I715044	.	CDS	1229	1699	.	+	0	ID=annotation1935357;Name=Coding Amino Portion GFP (aka GFP1);Parent=BBa_I715019
BBa_I715044	.	CDS	1742	1961	.	+	0	ID=BBa_I715023;Name=BBa_I715023
BBa_I715044	.	stop_codon	1956	1960	.	+	0	ID=annotation1935384;Name=(two in tandem);Parent=BBa_I715023
BBa_I715044	.	sequence_feature	1742	1742	.	+	0	ID=annotation1935382;Name=extra base (avoid frameshift);Parent=BBa_I715023
BBa_I715044	.	CDS	1743	1955	.	+	0	ID=annotation1935383;Name=Continue coding sequence;Parent=BBa_I715023
BBa_I715044	.	sequence_feature	1708	1733	.	+	0	ID=BBa_J44000;Name=BBa_J44000
BBa_I715044	.	CDS	983	1202	.	+	0	ID=BBa_I715023;Name=BBa_I715023
BBa_I715044	.	stop_codon	1197	1201	.	+	0	ID=annotation1935384;Name=(two in tandem);Parent=BBa_I715023
BBa_I715044	.	sequence_feature	983	983	.	+	0	ID=annotation1935382;Name=extra base (avoid frameshift);Parent=BBa_I715023
BBa_I715044	.	CDS	984	1196	.	+	0	ID=annotation1935383;Name=Continue coding sequence;Parent=BBa_I715023
BBa_I715044	.	terminator	2058	2098	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I715044	.	engineered_region	2058	2098	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I715044	.	stop_codon	2091	2091	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I715044	.	stem_loop	2065	2084	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I715044	.	polyA_site	2085	2098	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
>BBa_I715044
taatacgactcactatagggagatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagag
ttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtc
cgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtcccc
gcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaa
ggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacg
gtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatggg
ttgggaagcttccaccgaacgtatgtacccggaagactactagagttatcaaaaaccatggtttttgataatactagag
taagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaa
caaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaag
atcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaact
atacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctg
ttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagag
ttatcaaaaaccatggtttttgataatactagagtggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaaga
cggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaacc
gacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcact
ccaccggtgcttaataatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtc
ccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacat
acggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcgg
ttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggt
tatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgata
cccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaa
ctataactcacacaatgtatacatcatggcagacaaacaatactagagttatcaaaaaccatggtttttgataatacta
gagtggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaacc
acctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacg
aagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataatactagagccaggc
atcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctacta
gagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttatcaaaaaccatggtttttgataa