##gff-version 3
##sequence-region BBa_I715009 1 2991
BBa_I715009	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_I715009	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_I715009	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_I715009	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_I715009	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_I715009	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_I715009	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_I715009	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_I715009	.	engineered_region	209	2991	.	+	0	ID=BBa_S03723;Name=BBa_S03723
BBa_I715009	.	engineered_region	209	1042	.	+	0	ID=BBa_S03709;Name=BBa_S03709;Parent=BBa_S03723
BBa_I715009	.	CDS	227	1042	.	+	0	ID=BBa_J31003;Name=BBa_J31003;Parent=BBa_S03709
BBa_I715009	.	CDS	227	1042	.	+	0	ID=annotation1884996;Name=KanR Gene;Parent=BBa_J31003
BBa_I715009	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03709
BBa_I715009	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I715009	.	engineered_region	1051	2991	.	+	0	ID=BBa_S03720;Name=BBa_S03720;Parent=BBa_S03723
BBa_I715009	.	engineered_region	1051	2259	.	+	0	ID=BBa_S03710;Name=BBa_S03710;Parent=BBa_S03720
BBa_I715009	.	ribosome_entry_site	1051	1062	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03710
BBa_I715009	.	conserved_region	1055	1058	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I715009	.	CDS	1069	2259	.	+	0	ID=BBa_J31007;Name=BBa_J31007;Parent=BBa_S03710
BBa_I715009	.	primer_binding_site	1069	1092	.	+	0	ID=annotation1910571;Name=Fwd primer J31007;Parent=BBa_J31007
BBa_I715009	.	CDS	1069	2259	.	+	0	ID=annotation1885000;Name=tetA(C) forward;Parent=BBa_J31007
BBa_I715009	.	stop_codon	2257	2259	.	+	0	ID=annotation1910573;Name=stop;Parent=BBa_J31007
BBa_I715009	.	primer_binding_site	2240	2259	.	+	0	ID=annotation1910572;Name=Rev primer J31007;Parent=BBa_J31007
BBa_I715009	.	engineered_region	2268	2991	.	+	0	ID=BBa_I13502;Name=BBa_I13502;Parent=BBa_S03720
BBa_I715009	.	CDS	2286	2966	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13502
BBa_I715009	.	CDS	2286	2960	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_I715009	.	engineered_tag	2967	2991	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_I715009	.	ribosome_entry_site	2268	2279	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13502
BBa_I715009	.	conserved_region	2272	2275	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_I715009
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgagccatat
tcaacgggaaacgtcttgctccaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgc
gataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatg
gcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgac
catcaagcattttatccgtactcctgatgatgcatggttactcaccactgcgatccccgggaaaacagcattccaggta
ttagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctg
tttgtaattgtccttttaacagcgatcgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttga
tgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcataagcttttgccattc
tcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgta
ttgatgttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctcc
ttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctc
gatgagtttttctaatactagagaaagaggagaaatactagatgaaatctaacaatgcgctcatcgtcatcctcggcac
cgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgac
agcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcac
tgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgac
cacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcc
tatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatgg
tggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacgg
cctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttc
aacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaac
tcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcct
gtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggccccgccaccaaacgtttcggcgag
aagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatgg
ccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtaga
tgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcattggaccgctgatcgtc
acggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcc
tccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaatactagagaaagaggagaaatactagatggct
tcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaa
tcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgcc
gttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggac
tacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgtta
cccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtcc
ggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaa
atcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccgg
ttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaaca
gtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc