LOCUS       BBa_I715009             2991 bp    DNA     linear       20-Dec-2025
DEFINITION  Plac:RBS:Kan:RBS:Tet:RBS:RFP
ACCESSION   BBa_I715009
VERSION     BBa_I715009.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..1042
                     /label=KanR Gene
     CDS             227..1042
                     /label=BBa_J31003
     misc_feature    209..1042
                     /label=BBa_S03709
     misc_feature    1055..1058
                     /label=conserved
     RBS             1051..1062
                     /label=BBa_B0034
     CDS             1069..2259
                     /label=tetA(C) forward
     primer_bind     1069..1092
                     /label=Fwd primer J31007
     primer_bind     2240..2259
                     /label=Rev primer J31007
     misc_feature    2257..2259
                     /label=stop
     CDS             1069..2259
                     /label=BBa_J31007
     misc_feature    1051..2259
                     /label=BBa_S03710
     misc_feature    2272..2275
                     /label=conserved
     RBS             2268..2279
                     /label=BBa_B0034
     CDS             2286..2960
                     /label=mrfp1
     misc_feature    2967..2991
                     /label=Help:Barcodes
     CDS             2286..2966
                     /label=BBa_E1010
     misc_feature    2268..2991
                     /label=BBa_I13502
     misc_feature    1051..2991
                     /label=BBa_S03720
     misc_feature    209..2991
                     /label=BBa_S03723
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga gccatattca
      241 acgggaaacg tcttgctcca ggccgcgatt aaattccaac atggatgctg atttatatgg
      301 gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc gattgtatgg
      361 gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt
      421 tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa
      481 gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc ccgggaaaac
      541 agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc
      601 agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg
      661 cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga
      721 ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataagct
      781 tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat
      841 ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg
      901 ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa
      961 acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt
     1021 gatgctcgat gagtttttct aatactagag aaagaggaga aatactagat gaaatctaac
     1081 aatgcgctca tcgtcatcct cggcaccgtc accctggatg ctgtaggcat aggcttggtt
     1141 atgccggtac tgccgggcct cttgcgggat atcgtccatt ccgacagcat cgccagtcac
     1201 tatggcgtgc tgctagcgct atatgcgttg atgcaatttc tatgcgcacc cgttctcgga
     1261 gcactgtccg accgctttgg ccgccgccca gtcctgctcg cttcgctact tggagccact
     1321 atcgactacg cgatcatggc gaccacaccc gtcctgtgga tcctctacgc cggacgcatc
     1381 gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc
     1441 gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg
     1501 gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt
     1561 gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg
     1621 cataagggag agcgtcgacc gatgcccttg agagccttca acccagtcag ctccttccgg
     1681 tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat catgcaactc
     1741 gtaggacagg tgccggcagc gctctgggtc attttcggcg aggaccgctt tcgctggagc
     1801 gcgacgatga tcggcctgtc gcttgcggta ttcggaatct tgcacgccct cgctcaagcc
     1861 ttcgtcactg gccccgccac caaacgtttc ggcgagaagc aggccattat cgccggcatg
     1921 gcggccgacg cgctgggcta cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc
     1981 cccattatga ttcttctcgc ttccggcggc atcgggatgc ccgcgttgca ggccatgctg
     2041 tccaggcagg tagatgacga ccatcaggga cagcttcaag gatcgctcgc ggctcttacc
     2101 agcctaactt cgatcattgg accgctgatc gtcacggcga tttatgccgc ctcggcgagc
     2161 acatggaacg ggttggcatg gattgtaggc gccgccctat accttgtctg cctccccgcg
     2221 ttgcgtcgcg gtgcatggag ccgggccacc tcgacctaat actagagaaa gaggagaaat
     2281 actagatggc ttcctccgaa gacgttatca aagagttcat gcgtttcaaa gttcgtatgg
     2341 aaggttccgt taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg
     2401 aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg
     2461 acatcctgtc cccgcagttc cagtacggtt ccaaagctta cgttaaacac ccggctgaca
     2521 tcccggacta cctgaaactg tccttcccgg aaggtttcaa atgggaacgt gttatgaact
     2581 tcgaagacgg tggtgttgtt accgttaccc aggactcctc cctgcaagac ggtgagttca
     2641 tctacaaagt taaactgcgt ggtaccaact tcccgtccga cggtccggtt atgcagaaaa
     2701 aaaccatggg ttgggaagct tccaccgaac gtatgtaccc ggaagacggt gctctgaaag
     2761 gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca ctacgacgct gaagttaaaa
     2821 ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac
     2881 tggacatcac ctcccacaac gaagactaca ccatcgttga acagtacgaa cgtgctgaag
     2941 gtcgtcactc caccggtgct taataacgct gatagtgcta gtgtagatcg c
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