##gff-version 3
##sequence-region BBa_I6301 1 5579
BBa_I6301	.	engineered_region	3323	4693	.	+	0	ID=BBa_Q04121;Name=BBa_Q04121
BBa_I6301	.	ribosome_entry_site	3323	3334	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04121
BBa_I6301	.	conserved_region	3327	3330	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I6301	.	terminator	4502	4630	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_Q04121
BBa_I6301	.	terminator	4502	4581	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I6301	.	engineered_region	4502	4581	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I6301	.	stem_loop	4513	4556	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I6301	.	terminator	4590	4630	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I6301	.	engineered_region	4590	4630	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I6301	.	stop_codon	4623	4623	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I6301	.	polyA_site	4617	4630	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I6301	.	stem_loop	4597	4616	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I6301	.	CDS	3341	4468	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_Q04121
BBa_I6301	.	engineered_tag	4469	4493	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_I6301	.	CDS	3341	4468	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_I6301	.	tag	4430	4468	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_I6301	.	engineered_region	3341	4468	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_I6301	.	promoter	4639	4692	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_Q04121
BBa_I6301	.	promoter	4658	4663	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_I6301	.	operator	4641	4657	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_I6301	.	promoter	4681	4686	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_I6301	.	engineered_region	4639	4692	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_I6301	.	operator	4664	4680	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_I6301	.	engineered_region	1219	2096	.	+	0	ID=BBa_E0420;Name=BBa_E0420
BBa_I6301	.	terminator	2056	2096	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0420
BBa_I6301	.	engineered_region	2056	2096	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I6301	.	stop_codon	2089	2089	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I6301	.	polyA_site	2083	2096	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I6301	.	stem_loop	2063	2082	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I6301	.	ribosome_entry_site	1219	1230	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0420
BBa_I6301	.	conserved_region	1223	1226	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I6301	.	terminator	1968	2047	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0420
BBa_I6301	.	engineered_region	1968	2047	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I6301	.	stem_loop	1979	2022	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I6301	.	CDS	1237	1959	.	+	0	ID=BBa_E0020;Name=BBa_E0020;Parent=BBa_E0420
BBa_I6301	.	engineered_region	4702	5579	.	+	0	ID=BBa_E0430;Name=BBa_E0430
BBa_I6301	.	ribosome_entry_site	4702	4713	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0430
BBa_I6301	.	conserved_region	4706	4709	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I6301	.	terminator	5539	5579	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0430
BBa_I6301	.	engineered_region	5539	5579	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I6301	.	stop_codon	5572	5572	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I6301	.	polyA_site	5566	5579	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I6301	.	stem_loop	5546	5565	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I6301	.	CDS	4720	5442	.	+	0	ID=BBa_E0030;Name=BBa_E0030;Parent=BBa_E0430
BBa_I6301	.	terminator	5451	5530	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0430
BBa_I6301	.	engineered_region	5451	5530	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I6301	.	stem_loop	5462	5505	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I6301	.	promoter	1	1210	.	+	0	ID=BBa_I0500;Name=BBa_I0500
BBa_I6301	.	sequence_feature	1	1079	.	+	0	ID=annotation1498843;Name=AraC;Parent=BBa_I0500
BBa_I6301	.	promoter	1043	1114	.	+	0	ID=annotation1498842;Name=PC;Parent=BBa_I0500
BBa_I6301	.	promoter	1080	1210	.	+	0	ID=annotation1498841;Name=Pbad;Parent=BBa_I0500
BBa_I6301	.	promoter	2105	3314	.	+	0	ID=BBa_I0500;Name=BBa_I0500
BBa_I6301	.	sequence_feature	2105	3183	.	+	0	ID=annotation1498843;Name=AraC;Parent=BBa_I0500
BBa_I6301	.	promoter	3147	3218	.	+	0	ID=annotation1498842;Name=PC;Parent=BBa_I0500
BBa_I6301	.	promoter	3184	3314	.	+	0	ID=annotation1498841;Name=Pbad;Parent=BBa_I0500
>BBa_I6301
ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcat
tttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtgg
tgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaa
aagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtga
tcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgcc
gcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttg
cccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccag
ttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgac
cgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcac
caccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataa
ccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcg
cttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactg
cgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaa
gccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacac
tttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttc
tccatacccgtttttttgggctagctactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttca
ccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgaggg
cgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtg
accaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgcca
tgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagtt
cgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaag
ctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaaga
tccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgt
gctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtc
ctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcat
caaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactaga
gtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttatgacaacttgacggctacatcattca
ctttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttg
atcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgata
cgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagc
aaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtac
ccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatc
gccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggt
gcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcgg
acgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaac
agcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattga
gaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgc
caccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgcca
ttcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaacca
aaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgt
ctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccata
agattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagctact
agagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctct
tatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatgg
cggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctc
cagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtg
gtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggc
tgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttct
tgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctg
gtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggct
ggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttca
acaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgca
atgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagct
catgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgca
actctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgccc
aatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcg
ggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagcc
aggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctct
actagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattga
cattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatggtgagcaagggcgaggag
ctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagg
gcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccac
cctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaag
tccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgagg
tgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggg
gcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaac
ttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacg
gccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatca
catggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagc
caggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
tactagagtcacactggctcaccttcgggtgggcctttctgcgtttata