##gff-version 3
##sequence-region BBa_I6001 1 5657
BBa_I6001	.	engineered_region	3362	4732	.	+	0	ID=BBa_Q04121;Name=BBa_Q04121
BBa_I6001	.	ribosome_entry_site	3362	3373	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04121
BBa_I6001	.	conserved_region	3366	3369	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I6001	.	promoter	4678	4731	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_Q04121
BBa_I6001	.	promoter	4720	4725	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_I6001	.	operator	4680	4696	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_I6001	.	operator	4703	4719	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_I6001	.	engineered_region	4678	4731	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_I6001	.	promoter	4697	4702	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_I6001	.	terminator	4541	4669	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_Q04121
BBa_I6001	.	terminator	4541	4620	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I6001	.	stem_loop	4552	4595	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I6001	.	engineered_region	4541	4620	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I6001	.	terminator	4629	4669	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I6001	.	stop_codon	4662	4662	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I6001	.	engineered_region	4629	4669	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I6001	.	stem_loop	4636	4655	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I6001	.	polyA_site	4656	4669	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I6001	.	CDS	3380	4507	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_Q04121
BBa_I6001	.	engineered_region	3380	4507	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_I6001	.	engineered_tag	4508	4532	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_I6001	.	CDS	3380	4507	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_I6001	.	tag	4469	4507	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_I6001	.	promoter	2144	3353	.	+	0	ID=BBa_I0500;Name=BBa_I0500
BBa_I6001	.	sequence_feature	2144	3222	.	+	0	ID=annotation1498843;Name=AraC;Parent=BBa_I0500
BBa_I6001	.	promoter	3223	3353	.	+	0	ID=annotation1498841;Name=Pbad;Parent=BBa_I0500
BBa_I6001	.	promoter	3186	3257	.	+	0	ID=annotation1498842;Name=PC;Parent=BBa_I0500
BBa_I6001	.	engineered_region	4741	5657	.	+	0	ID=BBa_E0432;Name=BBa_E0432
BBa_I6001	.	terminator	5529	5608	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0432
BBa_I6001	.	stem_loop	5540	5583	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I6001	.	engineered_region	5529	5608	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I6001	.	terminator	5617	5657	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0432
BBa_I6001	.	stop_codon	5650	5650	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I6001	.	engineered_region	5617	5657	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I6001	.	stem_loop	5624	5643	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I6001	.	polyA_site	5644	5657	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I6001	.	ribosome_entry_site	4741	4752	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0432
BBa_I6001	.	conserved_region	4745	4748	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I6001	.	engineered_region	4759	5520	.	+	0	ID=BBa_E0032;Name=BBa_E0032;Parent=BBa_E0432
BBa_I6001	.	CDS	4759	5520	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_I6001	.	engineered_region	4759	5520	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_I6001	.	stop_codon	5515	5520	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_I6001	.	sequence_alteration	5000	5000	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_I6001	.	tag	5476	5514	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_I6001	.	promoter	1	1210	.	+	0	ID=BBa_I0500;Name=BBa_I0500
BBa_I6001	.	sequence_feature	1	1079	.	+	0	ID=annotation1498843;Name=AraC;Parent=BBa_I0500
BBa_I6001	.	promoter	1080	1210	.	+	0	ID=annotation1498841;Name=Pbad;Parent=BBa_I0500
BBa_I6001	.	promoter	1043	1114	.	+	0	ID=annotation1498842;Name=PC;Parent=BBa_I0500
BBa_I6001	.	engineered_region	1219	2135	.	+	0	ID=BBa_E0422;Name=BBa_E0422
BBa_I6001	.	terminator	2007	2086	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0422
BBa_I6001	.	stem_loop	2018	2061	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I6001	.	engineered_region	2007	2086	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I6001	.	ribosome_entry_site	1219	1230	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0422
BBa_I6001	.	conserved_region	1223	1226	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I6001	.	terminator	2095	2135	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0422
BBa_I6001	.	stop_codon	2128	2128	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I6001	.	engineered_region	2095	2135	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I6001	.	stem_loop	2102	2121	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I6001	.	polyA_site	2122	2135	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I6001	.	engineered_region	1237	1998	.	+	0	ID=BBa_E0022;Name=BBa_E0022;Parent=BBa_E0422
BBa_I6001	.	CDS	1237	1998	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_I6001	.	point_mutation	1305	1305	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_I6001	.	tag	1955	1992	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
BBa_I6001	.	stop_codon	1993	1998	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_I6001	.	engineered_region	1237	1998	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
>BBa_I6001
ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcat
tttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtgg
tgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaa
aagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtga
tcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgcc
gcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttg
cccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccag
ttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgac
cgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcac
caccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataa
ccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcg
cttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactg
cgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaa
gccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacac
tttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttc
tccatacccgtttttttgggctagctactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttca
ccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgaggg
cgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtg
accaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgcca
tgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagtt
cgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaag
ctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaaga
tccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgt
gctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtc
ctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaa
actacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttc
gttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgttta
tatactagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggc
cccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggc
atccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaact
gctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtc
tgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgct
tccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgt
taatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatat
tgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctcc
ggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccct
gatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaa
atcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcagggga
tcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacatt
gccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaag
cgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcac
ggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactct
ctactgtttctccatacccgtttttttgggctagctactagagaaagaggagaaatactagatggtgaatgtgaaacca
gtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacg
tttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaact
ggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcg
attaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaag
cggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgc
tgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttc
tcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcc
cattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagc
ggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccact
gcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcgg
atatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattt
tcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttg
cccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcat
taatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagctta
ataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactggg
cctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctg
cgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagag
aaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacg
gcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagtt
catctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcc
cgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatct
tcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagct
gaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctat
atcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagc
tcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctacca
gtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcact
ctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagcc
aggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctct
actagagtcacactggctcaccttcgggtgggcctttctgcgtttata