##gff-version 3
##sequence-region BBa_I5400 1 2732
BBa_I5400	.	terminator	2638	2678	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I5400	.	stem_loop	2645	2664	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I5400	.	engineered_region	2638	2678	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I5400	.	stop_codon	2671	2671	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I5400	.	polyA_site	2665	2678	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I5400	.	terminator	864	904	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I5400	.	stem_loop	871	890	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I5400	.	engineered_region	864	904	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I5400	.	stop_codon	897	897	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I5400	.	polyA_site	891	904	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I5400	.	promoter	967	1015	.	+	0	ID=BBa_R0051;Name=BBa_R0051
BBa_I5400	.	engineered_region	967	1015	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_I5400	.	promoter	981	986	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_I5400	.	non_covalent_binding_site	967	983	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_I5400	.	promoter	1004	1009	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_I5400	.	non_covalent_binding_site	991	1007	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_I5400	.	CDS	81	830	.	+	0	ID=BBa_C0051;Name=BBa_C0051
BBa_I5400	.	CDS	84	791	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_I5400	.	engineered_tag	831	855	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_I5400	.	tag	792	824	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_I5400	.	terminator	913	958	.	+	0	ID=BBa_B0011;Name=BBa_B0011
BBa_I5400	.	engineered_region	913	958	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_I5400	.	stem_loop	925	947	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_I5400	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I5400	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I5400	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I5400	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I5400	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I5400	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I5400	.	terminator	1811	1856	.	+	0	ID=BBa_B0011;Name=BBa_B0011
BBa_I5400	.	engineered_region	1811	1856	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_I5400	.	stem_loop	1823	1845	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_I5400	.	CDS	1042	1728	.	+	0	ID=BBa_C0053;Name=BBa_C0053
BBa_I5400	.	stop_codon	1723	1728	.	+	0	ID=annotation1751;Name=stop;Parent=BBa_C0053
BBa_I5400	.	tag	1690	1722	.	+	0	ID=annotation1750;Name=LVA;Parent=BBa_C0053
BBa_I5400	.	CDS	1042	1689	.	+	0	ID=annotation1747;Name=cII p22;Parent=BBa_C0053
BBa_I5400	.	engineered_tag	1729	1753	.	+	0	ID=annotation2213993;Name=Help:Barcodes;Parent=BBa_C0053
BBa_I5400	.	engineered_region	1042	1728	.	+	0	ID=annotation7036;Name=BBa_C0053;Parent=BBa_C0053
BBa_I5400	.	CDS	1945	2604	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_I5400	.	tag	2565	2598	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_I5400	.	engineered_tag	2605	2629	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_I5400	.	CDS	1948	2564	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_I5400	.	ribosome_entry_site	1024	1035	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I5400	.	conserved_region	1028	1031	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I5400	.	terminator	2687	2732	.	+	0	ID=BBa_B0011;Name=BBa_B0011
BBa_I5400	.	engineered_region	2687	2732	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_I5400	.	stem_loop	2699	2721	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_I5400	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I5400	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I5400	.	ribosome_entry_site	1927	1938	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I5400	.	conserved_region	1931	1934	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I5400	.	promoter	1865	1918	.	+	0	ID=BBa_R0053;Name=BBa_R0053
BBa_I5400	.	non_covalent_binding_site	1865	1867	.	+	0	ID=annotation2035;Name=OR3;Parent=BBa_R0053
BBa_I5400	.	promoter	1882	1887	.	+	0	ID=annotation2038;Name=-35;Parent=BBa_R0053
BBa_I5400	.	non_covalent_binding_site	1875	1892	.	+	0	ID=annotation2036;Name=OR2;Parent=BBa_R0053
BBa_I5400	.	non_covalent_binding_site	1898	1915	.	+	0	ID=annotation2037;Name=OR1;Parent=BBa_R0053
BBa_I5400	.	promoter	1894	1899	.	+	0	ID=annotation2042;Name=-10;Parent=BBa_R0053
BBa_I5400	.	engineered_region	1865	1918	.	+	0	ID=annotation7069;Name=BBa_R0053;Parent=BBa_R0053
BBa_I5400	.	promoter	1872	1877	.	+	0	ID=annotation2041;Name=-35;Parent=BBa_R0053
BBa_I5400	.	terminator	1762	1802	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I5400	.	stem_loop	1769	1788	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I5400	.	engineered_region	1762	1802	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I5400	.	stop_codon	1795	1795	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I5400	.	polyA_site	1789	1802	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
>BBa_I5400
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaag
aaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttattta
atggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagccc
ttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccct
gttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaa
gcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaa
gccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccaga
cttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagt
acccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttgg
cgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagtcacac
tggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttt
tttattattttactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaag
aggagaaatactagatgaatacacaattgatgggtgagcgtattcgcgctcgaagaaaaaaactcaagattagacaagc
cgctcttggtaagatggtgggagtgtctaatgttgcaatatcgcaatgggagcgctcggagactgagccaaatggggag
aacctgttggcactttcgaaggctcttcagtgctcccctgactatttgctgaaaggagatttaagccagacaaacgttg
cctatcatagtaggcatgagccaagaggatcataccctcttatcagttgggtaagcgcagggcaatggatggaagctgt
agaaccttatcacaagcgcgcgatagagaactggcacgacaccactgtagattgttcagaagattcattttggcttgat
gtccaaggtgactctatgacagcaccggcagggttaagcattccagaaggaatgataattctggttgatcccgaagtcg
aaccaagaaacggcaagctggttgttgcaaaattagaaggtgaaaacgaggccacattcaaaaaattagttatggatgc
aggccgaaagtttttaaaaccattaaacccacaatatccgatgatagaaatcaacggaaactgcaaaatcattggcgta
gttgttgacgcaaaactcgcaaatcttccagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagt
gctagtgtagatcactactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaat
ataaaaagccagattattaatccggcttttttattattttactagagaataaacttgactaaagattcctttagtagat
aatttaagtgttctttaatttctactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgatta
acagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtaga
gcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcac
catactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctt
tactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatca
attagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttacttta
ggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccat
tattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatg
cggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacac
tgatagtgctagtgtagatcactactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagag
agagaatataaaaagccagattattaatccggcttttttattattt