##gff-version 3
##sequence-region BBa_I5300 1 945
BBa_I5300	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I5300	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I5300	.	engineered_region	81	842	.	+	0	ID=BBa_E0022;Name=BBa_E0022
BBa_I5300	.	point_mutation	149	149	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_I5300	.	engineered_region	81	842	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
BBa_I5300	.	tag	799	836	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
BBa_I5300	.	CDS	81	842	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_I5300	.	stop_codon	837	842	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_I5300	.	terminator	851	891	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I5300	.	stop_codon	884	884	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I5300	.	polyA_site	878	891	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I5300	.	stem_loop	858	877	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I5300	.	engineered_region	851	891	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I5300	.	terminator	900	945	.	+	0	ID=BBa_B0011;Name=BBa_B0011
BBa_I5300	.	engineered_region	900	945	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_I5300	.	stem_loop	912	934	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_I5300	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I5300	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I5300	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I5300	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I5300	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I5300	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
>BBa_I5300
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccac
aagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggca
agctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacat
gaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggc
aactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttca
aggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagca
gaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccag
cagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaag
accccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagct
gtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagtcacactggctcaccttcg
ggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt