##gff-version 3 ##sequence-region BBa_I5230 1 3116 BBa_I5230 . terminator 824 869 . + 0 ID=BBa_B0011;Name=BBa_B0011 BBa_I5230 . stem_loop 836 858 . + 0 ID=annotation1683;Name=stem_loop;Parent=BBa_B0011 BBa_I5230 . engineered_region 824 869 . + 0 ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011 BBa_I5230 . CDS 1861 2988 . + 0 ID=BBa_C0012;Name=BBa_C0012 BBa_I5230 . engineered_tag 2989 3013 . + 0 ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012 BBa_I5230 . tag 2950 2988 . + 0 ID=annotation1722;Name=LVA;Parent=BBa_C0012 BBa_I5230 . engineered_region 1861 2988 . + 0 ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012 BBa_I5230 . CDS 1861 2988 . + 0 ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012 BBa_I5230 . promoter 1781 1834 . + 0 ID=BBa_R0053;Name=BBa_R0053 BBa_I5230 . non_covalent_binding_site 1791 1808 . + 0 ID=annotation2036;Name=OR2;Parent=BBa_R0053 BBa_I5230 . non_covalent_binding_site 1781 1783 . + 0 ID=annotation2035;Name=OR3;Parent=BBa_R0053 BBa_I5230 . promoter 1810 1815 . + 0 ID=annotation2042;Name=-10;Parent=BBa_R0053 BBa_I5230 . engineered_region 1781 1834 . + 0 ID=annotation7069;Name=BBa_R0053;Parent=BBa_R0053 BBa_I5230 . promoter 1798 1803 . + 0 ID=annotation2038;Name=-35;Parent=BBa_R0053 BBa_I5230 . promoter 1788 1793 . + 0 ID=annotation2041;Name=-35;Parent=BBa_R0053 BBa_I5230 . non_covalent_binding_site 1814 1831 . + 0 ID=annotation2037;Name=OR1;Parent=BBa_R0053 BBa_I5230 . terminator 1678 1718 . + 0 ID=BBa_B0012;Name=BBa_B0012 BBa_I5230 . stem_loop 1685 1704 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_I5230 . engineered_region 1678 1718 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_I5230 . stop_codon 1711 1711 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_I5230 . polyA_site 1705 1718 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_I5230 . promoter 1 54 . + 0 ID=BBa_R0011;Name=BBa_R0011 BBa_I5230 . operator 26 42 . + 0 ID=annotation2001;Name=lac O1;Parent=BBa_R0011 BBa_I5230 . engineered_region 1 54 . + 0 ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011 BBa_I5230 . operator 3 19 . + 0 ID=annotation1999;Name=lac O1;Parent=BBa_R0011 BBa_I5230 . promoter 20 25 . + 0 ID=annotation2000;Name=-35;Parent=BBa_R0011 BBa_I5230 . promoter 43 48 . + 0 ID=annotation2002;Name=-10;Parent=BBa_R0011 BBa_I5230 . promoter 878 931 . + 0 ID=BBa_R0040;Name=BBa_R0040 BBa_I5230 . promoter 897 902 . + 0 ID=annotation1986785;Name=-35;Parent=BBa_R0040 BBa_I5230 . non_covalent_binding_site 878 896 . + 0 ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040 BBa_I5230 . engineered_region 878 931 . + 0 ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040 BBa_I5230 . promoter 920 925 . + 0 ID=annotation1986787;Name=-10;Parent=BBa_R0040 BBa_I5230 . non_covalent_binding_site 903 921 . + 0 ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040 BBa_I5230 . ribosome_entry_site 1843 1854 . + 0 ID=BBa_B0034;Name=BBa_B0034 BBa_I5230 . conserved_region 1847 1850 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_I5230 . ribosome_entry_site 940 951 . + 0 ID=BBa_B0034;Name=BBa_B0034 BBa_I5230 . conserved_region 944 947 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_I5230 . ribosome_entry_site 64 75 . + 0 ID=BBa_B0034;Name=BBa_B0034 BBa_I5230 . conserved_region 68 71 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_I5230 . terminator 3071 3116 . + 0 ID=BBa_B0011;Name=BBa_B0011 BBa_I5230 . stem_loop 3083 3105 . + 0 ID=annotation1683;Name=stem_loop;Parent=BBa_B0011 BBa_I5230 . engineered_region 3071 3116 . + 0 ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011 BBa_I5230 . CDS 958 1644 . + 0 ID=BBa_C0053;Name=BBa_C0053 BBa_I5230 . engineered_tag 1645 1669 . + 0 ID=annotation2213993;Name=Help:Barcodes;Parent=BBa_C0053 BBa_I5230 . stop_codon 1639 1644 . + 0 ID=annotation1751;Name=stop;Parent=BBa_C0053 BBa_I5230 . tag 1606 1638 . + 0 ID=annotation1750;Name=LVA;Parent=BBa_C0053 BBa_I5230 . CDS 958 1605 . + 0 ID=annotation1747;Name=cII p22;Parent=BBa_C0053 BBa_I5230 . engineered_region 958 1644 . + 0 ID=annotation7036;Name=BBa_C0053;Parent=BBa_C0053 BBa_I5230 . terminator 3022 3062 . + 0 ID=BBa_B0012;Name=BBa_B0012 BBa_I5230 . stem_loop 3029 3048 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_I5230 . engineered_region 3022 3062 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_I5230 . stop_codon 3055 3055 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_I5230 . polyA_site 3049 3062 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_I5230 . terminator 775 815 . + 0 ID=BBa_B0012;Name=BBa_B0012 BBa_I5230 . stem_loop 782 801 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_I5230 . engineered_region 775 815 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_I5230 . stop_codon 808 808 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_I5230 . polyA_site 802 815 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_I5230 . CDS 82 766 . + 0 ID=BBa_C0040;Name=BBa_C0040 BBa_I5230 . CDS 85 701 . + 0 ID=annotation23329;Name=tetR;Parent=BBa_C0040 BBa_I5230 . engineered_tag 742 766 . + 0 ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040 BBa_I5230 . tag 702 735 . + 0 ID=annotation23330;Name=SsrA;Parent=BBa_C0040 BBa_I5230 . terminator 1727 1772 . + 0 ID=BBa_B0011;Name=BBa_B0011 BBa_I5230 . stem_loop 1739 1761 . + 0 ID=annotation1683;Name=stem_loop;Parent=BBa_B0011 BBa_I5230 . engineered_region 1727 1772 . + 0 ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011 >BBa_I5230 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact agatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaac aacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctc gacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttt tacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcc tacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcatta tatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaa gggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagcc agccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaac gacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagtcacactggctcacct tcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt tactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggag aaatactagatgaatacacaattgatgggtgagcgtattcgcgctcgaagaaaaaaactcaagattagacaagccgctc ttggtaagatggtgggagtgtctaatgttgcaatatcgcaatgggagcgctcggagactgagccaaatggggagaacct gttggcactttcgaaggctcttcagtgctcccctgactatttgctgaaaggagatttaagccagacaaacgttgcctat catagtaggcatgagccaagaggatcataccctcttatcagttgggtaagcgcagggcaatggatggaagctgtagaac cttatcacaagcgcgcgatagagaactggcacgacaccactgtagattgttcagaagattcattttggcttgatgtcca aggtgactctatgacagcaccggcagggttaagcattccagaaggaatgataattctggttgatcccgaagtcgaacca agaaacggcaagctggttgttgcaaaattagaaggtgaaaacgaggccacattcaaaaaattagttatggatgcaggcc gaaagtttttaaaaccattaaacccacaatatccgatgatagaaatcaacggaaactgcaaaatcattggcgtagttgt tgacgcaaaactcgcaaatcttccagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctag tgtagatcactactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaa aagccagattattaatccggcttttttattattttactagagaataaacttgactaaagattcctttagtagataattt aagtgttctttaatttctactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtc gcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcggg aaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgtt gctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgat caactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttc tcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcac taatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacg cgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcgg cgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactg gagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaac gatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggat acgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaac cagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaa agaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgac aggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgcta gtgtagatctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataa aaagccagattattaatccggcttttttattattt