##gff-version 3
##sequence-region BBa_I5220 1 3090
BBa_I5220	.	CDS	1835	2962	.	+	0	ID=BBa_C0012;Name=BBa_C0012
BBa_I5220	.	CDS	1835	2962	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_I5220	.	engineered_tag	2963	2987	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_I5220	.	engineered_region	1835	2962	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_I5220	.	tag	2924	2962	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_I5220	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I5220	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I5220	.	promoter	878	931	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I5220	.	engineered_region	878	931	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I5220	.	promoter	897	902	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I5220	.	promoter	920	925	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I5220	.	non_covalent_binding_site	878	896	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I5220	.	non_covalent_binding_site	903	921	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I5220	.	terminator	2996	3036	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I5220	.	stem_loop	3003	3022	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I5220	.	stop_codon	3029	3029	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I5220	.	polyA_site	3023	3036	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I5220	.	engineered_region	2996	3036	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I5220	.	terminator	1709	1754	.	+	0	ID=BBa_B0011;Name=BBa_B0011
BBa_I5220	.	engineered_region	1709	1754	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_I5220	.	stem_loop	1721	1743	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_I5220	.	CDS	82	766	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_I5220	.	tag	702	735	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_I5220	.	engineered_tag	742	766	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_I5220	.	CDS	85	701	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_I5220	.	terminator	1660	1700	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I5220	.	stem_loop	1667	1686	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I5220	.	stop_codon	1693	1693	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I5220	.	polyA_site	1687	1700	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I5220	.	engineered_region	1660	1700	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I5220	.	terminator	3045	3090	.	+	0	ID=BBa_B0011;Name=BBa_B0011
BBa_I5220	.	engineered_region	3045	3090	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_I5220	.	stem_loop	3057	3079	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_I5220	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_I5220	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_I5220	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_I5220	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_I5220	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_I5220	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_I5220	.	ribosome_entry_site	1817	1828	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I5220	.	conserved_region	1821	1824	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I5220	.	ribosome_entry_site	940	951	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I5220	.	conserved_region	944	947	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I5220	.	CDS	958	1626	.	+	0	ID=BBa_C0052;Name=BBa_C0052
BBa_I5220	.	engineered_region	958	1626	.	+	0	ID=annotation7035;Name=BBa_C0052;Parent=BBa_C0052
BBa_I5220	.	tag	1588	1626	.	+	0	ID=annotation1745;Name=LVA;Parent=BBa_C0052
BBa_I5220	.	CDS	958	1626	.	+	0	ID=annotation1743;Name=cI 434;Parent=BBa_C0052
BBa_I5220	.	engineered_tag	1627	1651	.	+	0	ID=annotation2213992;Name=Help:Barcodes;Parent=BBa_C0052
BBa_I5220	.	terminator	775	815	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I5220	.	stem_loop	782	801	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I5220	.	stop_codon	808	808	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I5220	.	polyA_site	802	815	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I5220	.	engineered_region	775	815	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I5220	.	terminator	824	869	.	+	0	ID=BBa_B0011;Name=BBa_B0011
BBa_I5220	.	engineered_region	824	869	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_I5220	.	stem_loop	836	858	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_I5220	.	promoter	1763	1808	.	+	0	ID=BBa_R0052;Name=BBa_R0052
BBa_I5220	.	promoter	1763	1768	.	+	0	ID=annotation2029;Name=-35;Parent=BBa_R0052
BBa_I5220	.	non_covalent_binding_site	1770	1783	.	+	0	ID=annotation2027;Name=OR2;Parent=BBa_R0052
BBa_I5220	.	promoter	1764	1769	.	+	0	ID=annotation2031;Name=-35;Parent=BBa_R0052
BBa_I5220	.	start_codon	1797	1799	.	+	0	ID=annotation2032;Name=start;Parent=BBa_R0052
BBa_I5220	.	engineered_region	1763	1808	.	+	0	ID=annotation7068;Name=BBa_R0052;Parent=BBa_R0052
BBa_I5220	.	non_covalent_binding_site	1792	1805	.	+	0	ID=annotation2028;Name=OR1;Parent=BBa_R0052
BBa_I5220	.	non_covalent_binding_site	1763	1805	.	+	0	ID=annotation2026;Name=OR2;Parent=BBa_R0052
BBa_I5220	.	promoter	1786	1791	.	+	0	ID=annotation2030;Name=-10;Parent=BBa_R0052
>BBa_I5220
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaac
aacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctc
gacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttt
tacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcc
tacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcatta
tatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaa
gggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagcc
agccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaac
gacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagtcacactggctcacct
tcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
tactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggag
aaatactagatgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaa
aggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgc
gtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgag
cccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaagg
acattgatgaatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttgaaggtgattccatgac
ctcacctgtaggtcaaagcatccctgaaggtcatatggtgttagtagatactggacgggagccagtgaatggaagcctt
gttgtagccaaactgactgacgcgaacgaagcaacattcaagaaactggtcatagatggcggtcagaagtacctgaaag
gcctgaatccttcatggcctatgactcctatcaacggaaactgcaagattatcggtgttgtcgtggaagcgagggtaaa
attcgtagctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagag
tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatcc
ggcttttttattattttactagagttgacaaacaagatacattgtatgaaaatacaagaaagtttgttgatactagaga
aagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatca
gaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggag
ctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtc
tggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtc
gatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatc
attaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatg
tctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgc
attgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcat
aaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaa
ccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcg
cgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgt
tatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactct
ctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatac
gcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcag
gctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagtcacact
ggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggctttt
ttattattt