##gff-version 3
##sequence-region BBa_I5210 1 3174
BBa_I5210	.	ribosome_entry_site	1901	1912	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I5210	.	conserved_region	1905	1908	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I5210	.	CDS	958	1732	.	+	0	ID=BBa_C0051;Name=BBa_C0051
BBa_I5210	.	CDS	961	1668	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_I5210	.	engineered_tag	1708	1732	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_I5210	.	tag	1669	1701	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_I5210	.	promoter	1844	1892	.	+	0	ID=BBa_R0051;Name=BBa_R0051
BBa_I5210	.	promoter	1858	1863	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_I5210	.	engineered_region	1844	1892	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_I5210	.	promoter	1881	1886	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_I5210	.	non_covalent_binding_site	1868	1884	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_I5210	.	non_covalent_binding_site	1844	1860	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_I5210	.	terminator	775	815	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I5210	.	engineered_region	775	815	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I5210	.	stop_codon	808	808	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I5210	.	polyA_site	802	815	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I5210	.	stem_loop	782	801	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I5210	.	terminator	3129	3174	.	+	0	ID=BBa_B0011;Name=BBa_B0011
BBa_I5210	.	stem_loop	3141	3163	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_I5210	.	engineered_region	3129	3174	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_I5210	.	CDS	1919	3046	.	+	0	ID=BBa_C0012;Name=BBa_C0012
BBa_I5210	.	engineered_tag	3047	3071	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_I5210	.	CDS	1919	3046	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_I5210	.	tag	3008	3046	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_I5210	.	engineered_region	1919	3046	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_I5210	.	CDS	82	766	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_I5210	.	tag	702	735	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_I5210	.	engineered_tag	742	766	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_I5210	.	CDS	85	701	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_I5210	.	promoter	878	931	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I5210	.	non_covalent_binding_site	903	921	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I5210	.	promoter	920	925	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I5210	.	engineered_region	878	931	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I5210	.	non_covalent_binding_site	878	896	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I5210	.	promoter	897	902	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I5210	.	ribosome_entry_site	940	951	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I5210	.	conserved_region	944	947	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I5210	.	terminator	824	869	.	+	0	ID=BBa_B0011;Name=BBa_B0011
BBa_I5210	.	stem_loop	836	858	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_I5210	.	engineered_region	824	869	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_I5210	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_I5210	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_I5210	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_I5210	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_I5210	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_I5210	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_I5210	.	terminator	1790	1835	.	+	0	ID=BBa_B0011;Name=BBa_B0011
BBa_I5210	.	stem_loop	1802	1824	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_I5210	.	engineered_region	1790	1835	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_I5210	.	terminator	3080	3120	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I5210	.	engineered_region	3080	3120	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I5210	.	stop_codon	3113	3113	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I5210	.	polyA_site	3107	3120	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I5210	.	stem_loop	3087	3106	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I5210	.	terminator	1741	1781	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I5210	.	engineered_region	1741	1781	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I5210	.	stop_codon	1774	1774	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I5210	.	polyA_site	1768	1781	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I5210	.	stem_loop	1748	1767	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I5210	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I5210	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_I5210
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaac
aacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctc
gacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttt
tacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcc
tacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcatta
tatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaa
gggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagcc
agccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaac
gacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagtcacactggctcacct
tcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
tactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggag
aaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatg
aaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgc
tttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaa
tttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatg
agtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagag
atgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaaca
ggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgca
tagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaa
cccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagag
acgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactag
agtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaat
ccggcttttttattattttactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgctact
agagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctct
tatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatgg
cggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctc
cagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtg
gtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggc
tgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttct
tgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctg
gtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggct
ggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttca
acaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgca
atgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagct
catgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgca
actctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgccc
aatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcg
ggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagtc
acactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccgg
cttttttattattt