##gff-version 3
##sequence-region BBa_I52000 1 1098
BBa_I52000	.	engineered_region	1	416	.	+	0	ID=BBa_P1011;Name=BBa_P1011
BBa_I52000	.	promoter	41	46	.	+	0	ID=annotation1822230;Name=-35;Parent=BBa_P1011
BBa_I52000	.	promoter	64	69	.	+	0	ID=annotation1822231;Name=-10;Parent=BBa_P1011
BBa_I52000	.	CDS	108	416	.	+	0	ID=annotation1822232;Name=ccdB;Parent=BBa_P1011
BBa_I52000	.	plasmid	425	1098	.	+	0	ID=BBa_I50020;Name=BBa_I50020
BBa_I52000	.	promoter	606	611	.	+	0	ID=annotation1793683;Name=RNAI transcript promoter -10;Parent=BBa_I50020
BBa_I52000	.	sequence_feature	487	1039	.	+	0	ID=annotation1793681;Name=RNAII transcript;Parent=BBa_I50020
BBa_I52000	.	sequence_alteration	727	727	.	+	0	ID=annotation1910690;Name=C to T, nonfunctional mutation;Parent=BBa_I50020
BBa_I52000	.	promoter	451	456	.	+	0	ID=annotation1793686;Name=RNAII transcript promoter -35 sequence;Parent=BBa_I50020
BBa_I52000	.	sequence_feature	440	1054	.	+	0	ID=annotation1782180;Name=rep (pMB1);Parent=BBa_I50020
BBa_I52000	.	sequence_feature	490	597	.	+	0	ID=annotation1793684;Name=RNAI transcript;Parent=BBa_I50020
BBa_I52000	.	sequence_alteration	698	698	.	+	0	ID=annotation1910689;Name=G to A, nonfunctional mutation;Parent=BBa_I50020
BBa_I52000	.	sequence_alteration	689	689	.	+	0	ID=annotation1910688;Name=G to A, nonfunctional mutation;Parent=BBa_I50020
BBa_I52000	.	promoter	472	477	.	+	0	ID=annotation1793685;Name=RNAII transcript promoter -10 sequence;Parent=BBa_I50020
BBa_I52000	.	sequence_feature	1039	1039	.	+	0	ID=annotation1782181;Name=ORI;Parent=BBa_I50020
BBa_I52000	.	promoter	628	633	.	+	0	ID=annotation1793682;Name=RNAI transcript promoter -35;Parent=BBa_I50020
BBa_I52000	.	sequence_alteration	685	685	.	+	0	ID=annotation1910687;Name=G to A, nonfunctional mutation;Parent=BBa_I50020
BBa_I52000	.	sequence_feature	451	1039	.	+	0	ID=annotation1793680;Name=origin of replication;Parent=BBa_I50020
BBa_I52000	.	sequence_alteration	891	891	.	+	0	ID=annotation1910691;Name=C to T, nonfunctional mutation;Parent=BBa_I50020
>BBa_I52000
ggcttactaaaagccagataacagtatgcgtatttgcgcgctgatttttgcggtataagaatatatactgatatgtata
cccgaagtatgtcaaaaagaggtatgctatgcagtttaaggtttacacctataaacgtgagagccgttatcgtctgttt
gtggacgtacagagtgatattattgacacgcctgggcgtcgcatggtgattcccctggccagcgcacgtctgctttcag
ataaagtctcccgtgaactttacccggtggtgcatatcggggacgaaagctggcgcatgatgaccaccgatatggccag
cgtgccggtttccgtgatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctg
atgttctggggaatataataatactagagcccgtagaaaagatcaaaagatcttcttgagatcctttttttctgcgcgt
aatctgctacttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactcttttt
ccgaaggtaactggctttagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttca
agaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtagctactgccagtagcgataagtcgtg
tcttaccgggttggattcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacag
cccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaag
ggagaaaggcggacaggtatctggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgc
ctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcgg
agcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacat