##gff-version 3
##sequence-region BBa_I5200 1 3174
BBa_I5200	.	terminator	3129	3174	.	+	0	ID=BBa_B0011;Name=BBa_B0011
BBa_I5200	.	stem_loop	3141	3163	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_I5200	.	engineered_region	3129	3174	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_I5200	.	CDS	1919	3046	.	+	0	ID=BBa_C0012;Name=BBa_C0012
BBa_I5200	.	engineered_region	1919	3046	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_I5200	.	tag	3008	3046	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_I5200	.	engineered_tag	3047	3071	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_I5200	.	CDS	1919	3046	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_I5200	.	terminator	1735	1775	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I5200	.	polyA_site	1762	1775	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I5200	.	engineered_region	1735	1775	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I5200	.	stop_codon	1768	1768	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I5200	.	stem_loop	1742	1761	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I5200	.	terminator	3080	3120	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I5200	.	polyA_site	3107	3120	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I5200	.	engineered_region	3080	3120	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I5200	.	stop_codon	3113	3113	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I5200	.	stem_loop	3087	3106	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I5200	.	terminator	1784	1829	.	+	0	ID=BBa_B0011;Name=BBa_B0011
BBa_I5200	.	stem_loop	1796	1818	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_I5200	.	engineered_region	1784	1829	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_I5200	.	terminator	824	869	.	+	0	ID=BBa_B0011;Name=BBa_B0011
BBa_I5200	.	stem_loop	836	858	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_I5200	.	engineered_region	824	869	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_I5200	.	ribosome_entry_site	940	951	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I5200	.	conserved_region	944	947	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I5200	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I5200	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I5200	.	promoter	878	931	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I5200	.	non_covalent_binding_site	903	921	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I5200	.	promoter	920	925	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I5200	.	promoter	897	902	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I5200	.	engineered_region	878	931	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I5200	.	non_covalent_binding_site	878	896	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I5200	.	promoter	1838	1892	.	+	0	ID=BBa_R0050;Name=BBa_R0050
BBa_I5200	.	ribosome_entry_site	1874	1888	.	+	0	ID=annotation2015;Name=OR1;Parent=BBa_R0050
BBa_I5200	.	promoter	1845	1850	.	+	0	ID=annotation2012;Name=-35;Parent=BBa_R0050
BBa_I5200	.	start_codon	1880	1880	.	+	0	ID=annotation2018;Name=putative;Parent=BBa_R0050
BBa_I5200	.	promoter	1867	1872	.	+	0	ID=annotation2014;Name=-10;Parent=BBa_R0050
BBa_I5200	.	engineered_region	1838	1892	.	+	0	ID=annotation7066;Name=BBa_R0050;Parent=BBa_R0050
BBa_I5200	.	ribosome_entry_site	1850	1864	.	+	0	ID=annotation2013;Name=OR2;Parent=BBa_R0050
BBa_I5200	.	CDS	82	766	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_I5200	.	tag	702	735	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_I5200	.	CDS	85	701	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_I5200	.	engineered_tag	742	766	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_I5200	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_I5200	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_I5200	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_I5200	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_I5200	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_I5200	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_I5200	.	ribosome_entry_site	1901	1912	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I5200	.	conserved_region	1905	1908	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I5200	.	CDS	958	1701	.	+	0	ID=BBa_C0050;Name=BBa_C0050
BBa_I5200	.	non_covalent_binding_site	958	965	.	+	0	ID=annotation1737;Name=OR3 partial;Parent=BBa_C0050
BBa_I5200	.	stop_codon	1696	1701	.	+	0	ID=annotation1734;Name=2;Parent=BBa_C0050
BBa_I5200	.	engineered_region	958	1701	.	+	0	ID=annotation7033;Name=BBa_C0050;Parent=BBa_C0050
BBa_I5200	.	CDS	958	1701	.	+	0	ID=annotation1735;Name=cI HK022;Parent=BBa_C0050
BBa_I5200	.	engineered_tag	1702	1726	.	+	0	ID=annotation2213990;Name=Help:Barcodes;Parent=BBa_C0050
BBa_I5200	.	tag	1663	1695	.	+	0	ID=annotation1736;Name=SsrA;Parent=BBa_C0050
BBa_I5200	.	terminator	775	815	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I5200	.	polyA_site	802	815	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I5200	.	engineered_region	775	815	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I5200	.	stop_codon	808	808	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I5200	.	stem_loop	782	801	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
>BBa_I5200
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaac
aacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctc
gacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttt
tacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcc
tacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcatta
tatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaa
gggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagcc
agccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaac
gacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagtcacactggctcacct
tcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
tactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggag
aaatactagatggttcaacagaaagagcgtgaaactttctcgcagaggcttgcgctggcctgtgataaagcgggattac
ctttgcatggtaggcaggctgatttagctgtcaggcttaaggtcacaccaaaagccattagtaaatggttcaacgggga
gtcaataccaagaaaagacaagatggaatctctggcttcggtgctgggaactactgctgcatatctgcatggctatgct
gatgatgacggtatcacggtaaatcatctatcaagatcaaatgattattatcgtgttgatgtattggatgttcaggcga
gcgccgggccaggaaccatggtttccaatgaatttatagaaaagataagagcaattgaatatacgaccgagcaggcaag
aattttatttaatggaaggccacaggaaagcgtaaaagtcatcacggttcgcggtgacagcatggagggaaccatcaat
ccgggagatgagatctttgttgatgtatccataacctgttttgatggcgatggcatttatgtgtttgtatacgggaaaa
caatgcacgttaagcgcctgcaaatgcaaaagaacaggcttgccgtcatctctgacaatgccgcttatgatcgatggta
catagaagaaggtgaagaagagcaacttcacattctagccaaagtcctcattaggcagtcaatcgattacaagcgattc
ggagctgcaaacgacgaaaactacgctttagtagcttaataaccctgatagtgctagtgtagatccctactagagtcac
actggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggct
tttttattattttactagagttaggtattgactgtactatcagttccgtcataatatgaaccataagttcaccactact
agagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctct
tatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatgg
cggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctc
cagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtg
gtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggc
tgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttct
tgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctg
gtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggct
ggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttca
acaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgca
atgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagct
catgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgca
actctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgccc
aatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcg
ggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagtc
acactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccgg
cttttttattattt