##gff-version 3
##sequence-region BBa_I5130 1 945
BBa_I5130	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I5130	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I5130	.	promoter	1	54	.	+	0	ID=BBa_R0053;Name=BBa_R0053
BBa_I5130	.	promoter	8	13	.	+	0	ID=annotation2041;Name=-35;Parent=BBa_R0053
BBa_I5130	.	non_covalent_binding_site	1	3	.	+	0	ID=annotation2035;Name=OR3;Parent=BBa_R0053
BBa_I5130	.	engineered_region	1	54	.	+	0	ID=annotation7069;Name=BBa_R0053;Parent=BBa_R0053
BBa_I5130	.	promoter	30	35	.	+	0	ID=annotation2042;Name=-10;Parent=BBa_R0053
BBa_I5130	.	promoter	18	23	.	+	0	ID=annotation2038;Name=-35;Parent=BBa_R0053
BBa_I5130	.	non_covalent_binding_site	34	51	.	+	0	ID=annotation2037;Name=OR1;Parent=BBa_R0053
BBa_I5130	.	non_covalent_binding_site	11	28	.	+	0	ID=annotation2036;Name=OR2;Parent=BBa_R0053
BBa_I5130	.	terminator	851	891	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I5130	.	polyA_site	878	891	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I5130	.	stem_loop	858	877	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I5130	.	engineered_region	851	891	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I5130	.	stop_codon	884	884	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I5130	.	engineered_region	81	842	.	+	0	ID=BBa_E0032;Name=BBa_E0032
BBa_I5130	.	stop_codon	837	842	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_I5130	.	sequence_alteration	322	322	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_I5130	.	engineered_region	81	842	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_I5130	.	CDS	81	842	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_I5130	.	tag	798	836	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_I5130	.	terminator	900	945	.	+	0	ID=BBa_B0011;Name=BBa_B0011
BBa_I5130	.	engineered_region	900	945	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_I5130	.	stem_loop	912	934	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
>BBa_I5130
aataaacttgactaaagattcctttagtagataatttaagtgttctttaatttctactagagaaagaggagaaatacta
gatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccac
aagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggca
agctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacat
gaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggc
aactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttca
aggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagca
gaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccag
cagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaag
accccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagct
gtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagtcacactggctcaccttcg
ggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt