##gff-version 3 ##sequence-region BBa_I51000 1 2446 BBa_I51000 . terminator 1132 1203 . + 0 ID=BBa_B0053;Name=BBa_B0053 BBa_I51000 . stem_loop 1140 1174 . + 0 ID=annotation318602;Name=stem_loop;Parent=BBa_B0053 BBa_I51000 . primer 1204 1223 . + 0 ID=BBa_G00100;Name=BBa_G00100 BBa_I51000 . primer_binding_site 1204 1223 . + 0 ID=annotation1934505;Name=VF2;Parent=BBa_G00100 BBa_I51000 . sequence_feature 177 182 . + 0 ID=BBa_B0045;Name=BBa_B0045 BBa_I51000 . sequence_feature 177 182 . + 0 ID=annotation1822724;Name=NheI site;Parent=BBa_B0045 BBa_I51000 . sequence_feature 116 135 . + 0 ID=BBa_G00102;Name=BBa_G00102 BBa_I51000 . primer_binding_site 116 135 . + 0 ID=annotation1961229;Name=VR;Parent=BBa_G00102 BBa_I51000 . terminator 47 115 . + 0 ID=BBa_B0054;Name=BBa_B0054 BBa_I51000 . sequence_feature 50 89 . + 0 ID=annotation1783342;Name=identical to E. coli MG1655 between tonB and yciA;Parent=BBa_B0054 BBa_I51000 . stem_loop 57 88 . + 0 ID=annotation1747359;Name=stem loop;Parent=BBa_B0054 BBa_I51000 . sequence_feature 1126 1131 . + 0 ID=BBa_B0045;Name=BBa_B0045 BBa_I51000 . sequence_feature 1126 1131 . + 0 ID=annotation1822724;Name=NheI site;Parent=BBa_B0045 BBa_I51000 . engineered_region 1349 2446 . + 0 ID=BBa_I52000;Name=BBa_I52000 BBa_I51000 . plasmid 1773 2446 . + 0 ID=BBa_I50020;Name=BBa_I50020;Parent=BBa_I52000 BBa_I51000 . sequence_alteration 2046 2046 . + 0 ID=annotation1910689;Name=G to A, nonfunctional mutation;Parent=BBa_I50020 BBa_I51000 . promoter 1976 1981 . + 0 ID=annotation1793682;Name=RNAI transcript promoter -35;Parent=BBa_I50020 BBa_I51000 . sequence_alteration 2033 2033 . + 0 ID=annotation1910687;Name=G to A, nonfunctional mutation;Parent=BBa_I50020 BBa_I51000 . promoter 1820 1825 . + 0 ID=annotation1793685;Name=RNAII transcript promoter -10 sequence;Parent=BBa_I50020 BBa_I51000 . sequence_feature 1838 1945 . + 0 ID=annotation1793684;Name=RNAI transcript;Parent=BBa_I50020 BBa_I51000 . promoter 1954 1959 . + 0 ID=annotation1793683;Name=RNAI transcript promoter -10;Parent=BBa_I50020 BBa_I51000 . sequence_alteration 2075 2075 . + 0 ID=annotation1910690;Name=C to T, nonfunctional mutation;Parent=BBa_I50020 BBa_I51000 . sequence_feature 1835 2387 . + 0 ID=annotation1793681;Name=RNAII transcript;Parent=BBa_I50020 BBa_I51000 . sequence_alteration 2239 2239 . + 0 ID=annotation1910691;Name=C to T, nonfunctional mutation;Parent=BBa_I50020 BBa_I51000 . sequence_feature 1788 2402 . + 0 ID=annotation1782180;Name=rep (pMB1);Parent=BBa_I50020 BBa_I51000 . sequence_feature 1799 2387 . + 0 ID=annotation1793680;Name=origin of replication;Parent=BBa_I50020 BBa_I51000 . sequence_feature 2387 2387 . + 0 ID=annotation1782181;Name=ORI;Parent=BBa_I50020 BBa_I51000 . promoter 1799 1804 . + 0 ID=annotation1793686;Name=RNAII transcript promoter -35 sequence;Parent=BBa_I50020 BBa_I51000 . sequence_alteration 2037 2037 . + 0 ID=annotation1910688;Name=G to A, nonfunctional mutation;Parent=BBa_I50020 BBa_I51000 . engineered_region 1349 1764 . + 0 ID=BBa_P1011;Name=BBa_P1011;Parent=BBa_I52000 BBa_I51000 . promoter 1412 1417 . + 0 ID=annotation1822231;Name=-10;Parent=BBa_P1011 BBa_I51000 . promoter 1389 1394 . + 0 ID=annotation1822230;Name=-35;Parent=BBa_P1011 BBa_I51000 . CDS 1456 1764 . + 0 ID=annotation1822232;Name=ccdB;Parent=BBa_P1011 BBa_I51000 . sequence_feature 1 21 . + 0 ID=BBa_G00001;Name=BBa_G00001 BBa_I51000 . sequence_feature 16 21 . + 0 ID=annotation1934227;Name=PstI;Parent=BBa_G00001 BBa_I51000 . sequence_feature 9 16 . + 0 ID=annotation1934228;Name=NotI;Parent=BBa_G00001 BBa_I51000 . sequence_feature 2 7 . + 0 ID=annotation1934226;Name=SpeI;Parent=BBa_G00001 BBa_I51000 . engineered_region 1 21 . + 0 ID=annotation1821544;Name=Suffix;Parent=BBa_G00001 BBa_I51000 . terminator 1224 1301 . + 0 ID=BBa_B0055;Name=BBa_B0055 BBa_I51000 . stem_loop 1259 1280 . + 0 ID=annotation1783301;Name=stem loop from bacteriophage T3;Parent=BBa_B0055 BBa_I51000 . sequence_feature 1251 1289 . + 0 ID=annotation1783311;Name=identical to bacteriophage T3;Parent=BBa_B0055 BBa_I51000 . terminator 136 176 . + 0 ID=BBa_B0062;Name=BBa_B0062 BBa_I51000 . stem_loop 149 168 . + 0 ID=annotation318637;Name=stem_loop;Parent=BBa_B0062 BBa_I51000 . sequence_feature 1302 1313 . + 0 ID=BBa_B0042;Name=BBa_B0042 BBa_I51000 . stop_codon 1311 1313 . + 0 ID=annotation1797086;Name=stop codon (+1 frame);Parent=BBa_B0042 BBa_I51000 . stop_codon 1306 1308 . + 0 ID=annotation1797088;Name=stop codon (-3 frame);Parent=BBa_B0042 BBa_I51000 . stop_codon 1310 1312 . + 0 ID=annotation1797087;Name=stop codon (-2 frame);Parent=BBa_B0042 BBa_I51000 . stop_codon 1303 1305 . + 0 ID=annotation1797083;Name=stop codon (+2 frame);Parent=BBa_B0042 BBa_I51000 . stop_codon 1307 1309 . + 0 ID=annotation1797085;Name=stop codon (+3 frame);Parent=BBa_B0042 BBa_I51000 . stop_codon 1302 1304 . + 0 ID=annotation1797084;Name=stop codon (-1 frame);Parent=BBa_B0042 BBa_I51000 . sequence_feature 22 34 . + 0 ID=BBa_B0044;Name=BBa_B0044 BBa_I51000 . sequence_feature 22 26 . + 0 ID=annotation1797126;Name=Nt.BstNB I recognition site;Parent=BBa_B0044 BBa_I51000 . sequence_feature 30 30 . + 0 ID=annotation1797129;Name=Generated T overhang;Parent=BBa_B0044 BBa_I51000 . sequence_feature 30 34 . + 0 ID=annotation1797128;Name=Topoisomerase I recognition site;Parent=BBa_B0044 BBa_I51000 . sequence_feature 27 29 . + 0 ID=annotation1797127;Name=Arbitrary sequence (could be any bases);Parent=BBa_B0044 BBa_I51000 . sequence_feature 35 46 . + 0 ID=BBa_B0042;Name=BBa_B0042 BBa_I51000 . stop_codon 44 46 . + 0 ID=annotation1797086;Name=stop codon (+1 frame);Parent=BBa_B0042 BBa_I51000 . stop_codon 39 41 . + 0 ID=annotation1797088;Name=stop codon (-3 frame);Parent=BBa_B0042 BBa_I51000 . stop_codon 43 45 . + 0 ID=annotation1797087;Name=stop codon (-2 frame);Parent=BBa_B0042 BBa_I51000 . stop_codon 36 38 . + 0 ID=annotation1797083;Name=stop codon (+2 frame);Parent=BBa_B0042 BBa_I51000 . stop_codon 40 42 . + 0 ID=annotation1797085;Name=stop codon (+3 frame);Parent=BBa_B0042 BBa_I51000 . stop_codon 35 37 . + 0 ID=annotation1797084;Name=stop codon (-1 frame);Parent=BBa_B0042 BBa_I51000 . sequence_feature 1327 1348 . + 0 ID=BBa_G00000;Name=BBa_G00000 BBa_I51000 . sequence_feature 1342 1347 . + 0 ID=annotation1934224;Name=XbaI site;Parent=BBa_G00000 BBa_I51000 . sequence_feature 1333 1340 . + 0 ID=annotation1961228;Name=NotI site;Parent=BBa_G00000 BBa_I51000 . engineered_region 1327 1348 . + 0 ID=annotation1934225;Name=Prefix;Parent=BBa_G00000 BBa_I51000 . sequence_feature 1327 1332 . + 0 ID=annotation1934223;Name=EcoRI site;Parent=BBa_G00000 BBa_I51000 . sequence_feature 1314 1326 . + 0 ID=BBa_B0043;Name=BBa_B0043 BBa_I51000 . sequence_feature 1318 1318 . + 0 ID=annotation1797104;Name=Generated T overhang;Parent=BBa_B0043 BBa_I51000 . sequence_feature 1314 1318 . + 0 ID=annotation1797101;Name=Topoisomerase I recognition site;Parent=BBa_B0043 BBa_I51000 . sequence_feature 1322 1326 . + 0 ID=annotation1797102;Name=Nt.BstNB I recognition site;Parent=BBa_B0043 BBa_I51000 . sequence_feature 1319 1321 . + 0 ID=annotation1797103;Name=Arbitrary sequence (could be any bases);Parent=BBa_B0043 BBa_I51000 . engineered_region 183 1125 . + 0 ID=BBa_P1006;Name=BBa_P1006 BBa_I51000 . promoter 1090 1095 . + 0 ID=annotation1897234;Name=ampR -35;Parent=BBa_P1006 BBa_I51000 . ribosome_entry_site 1051 1055 . + 0 ID=annotation1897232;Name=ampR RBS;Parent=BBa_P1006 BBa_I51000 . promoter 1064 1069 . + 0 ID=annotation1897233;Name=ampR -10;Parent=BBa_P1006 BBa_I51000 . CDS 183 1046 . + 0 ID=annotation1897231;Name=ampicillin resistance;Parent=BBa_P1006 >BBa_I51000 tactagtagcggccgctgcaggagtcactaagggttagttagttagattagcagaaagtcaaaagcctccgaccggagg cttttgactaaaacttcccttggggttatcattggggctcactcaaaggcggtaatcagataaaaaaaatccttagctt tcgctaaggatgatttctgctagcttattaccaatgcttaatcagggaggcacctatctcagcgatctggcgattacgt tcgtccatagttgcctggctccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagggctgcaa taataccgcgggacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaag tggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagcgagagtaagaagttcgccagttaag agtttgcgcaacgttgttgccatagctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccg gttcccaacgatcaaggcgagttacatgatcacccatgttgtgcaaaaaagcggtaagctccttcggtcctccgatcgt tgtcagaagtaagttggccgccgtgttatcactcatggttatggcagcgctgcataattcgcgtactgtcatgccgtcc gtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctctt gcccggcgtcaatacgggataataccgcgccacagagcagaactttaaaagtgctcatcattgggaaacgttcttcggg gcgaaaactctcaagaatcttaccgctgttgaggtccagttcgatgtaacccacacgtgcacccaactgatcttcggca tcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacac ggaaatgttgaatactcatattcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggata gatatttgaatgtatttaggctagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccg aaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggccca cccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttc tagagggcttactaaaagccagataacagtatgcgtatttgcgcgctgatttttgcggtataagaatatatactgatat gtatacccgaagtatgtcaaaaagaggtatgctatgcagtttaaggtttacacctataaacgtgagagccgttatcgtc tgtttgtggacgtacagagtgatattattgacacgcctgggcgtcgcatggtgattcccctggccagcgcacgtctgct ttcagataaagtctcccgtgaactttacccggtggtgcatatcggggacgaaagctggcgcatgatgaccaccgatatg gccagcgtgccggtttccgtgatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccatta acctgatgttctggggaatataataatactagagcccgtagaaaagatcaaaagatcttcttgagatcctttttttctg cgcgtaatctgctacttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactc tttttccgaaggtaactggctttagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccacca cttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtagctactgccagtagcgataag tcgtgtcttaccgggttggattcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgca cacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcc cgaagggagaaaggcggacaggtatctggtaagcggcagggtcggaacaggagagcgcacgagggagcttccaggggga aacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcagggg ggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacat