##gff-version 3
##sequence-region BBa_I50052 46 2002
BBa_I50052	.	stem_loop	46	75	.	+	0	ID=annotation2071336;Name=Terminator
BBa_I50052	.	sequence_alteration	1051	1053	.	+	0	ID=annotation2071357;Name=GCT->GTT ts mutation (Ala->Val)
BBa_I50052	.	polyA_site	1947	1962	.	+	0	ID=annotation2071359;Name=putative polyA
BBa_I50052	.	sequence_feature	139	152	.	+	0	ID=annotation2071338;Name=inverted repeat
BBa_I50052	.	sequence_alteration	1655	1655	.	+	0	ID=annotation2071358;Name=G to A difference with canonical pSC101 vector sequence
BBa_I50052	.	sequence_feature	862	884	.	+	0	ID=annotation2071352;Name=inverted repeat
BBa_I50052	.	ribosome_entry_site	870	882	.	+	0	ID=annotation2071353;Name=RBS
BBa_I50052	.	sequence_feature	158	162	.	+	0	ID=annotation2071339;Name=Tn3 integration site
BBa_I50052	.	sequence_alteration	1912	1912	.	+	0	ID=annotation2071361;Name=G to C difference with canonical pSC101 vector sequence
BBa_I50052	.	sequence_alteration	2002	2002	.	+	0	ID=annotation2071362;Name=T to C difference with canonical pSC101 vector sequence
BBa_I50052	.	CDS	886	1836	.	+	0	ID=annotation2071355;Name=repA
BBa_I50052	.	sequence_feature	192	215	.	+	0	ID=annotation2071341;Name=inverted repeat
BBa_I50052	.	sequence_alteration	814	819	.	+	0	ID=annotation2071347;Name=mutated SpeI site
BBa_I50052	.	sequence_feature	165	181	.	+	0	ID=annotation2071340;Name=inverted repeat
BBa_I50052	.	sequence_feature	838	859	.	+	0	ID=annotation2071349;Name=inverted repeat
BBa_I50052	.	sequence_feature	137	504	.	+	0	ID=annotation2071337;Name=par region
BBa_I50052	.	promoter	828	833	.	+	0	ID=annotation2071348;Name=-35
BBa_I50052	.	promoter	846	865	.	+	0	ID=annotation2071350;Name=promoter region
BBa_I50052	.	sequence_feature	635	717	.	+	0	ID=annotation2071343;Name=AT rich region
BBa_I50052	.	ribosome_entry_site	879	884	.	+	0	ID=annotation2071354;Name=RBS
BBa_I50052	.	sequence_feature	221	238	.	+	0	ID=annotation2071342;Name=inverted repeat
BBa_I50052	.	sequence_feature	769	788	.	+	0	ID=annotation2071346;Name=direct repeat
BBa_I50052	.	sequence_feature	716	735	.	+	0	ID=annotation2071344;Name=direct repeat
BBa_I50052	.	promoter	850	855	.	+	0	ID=annotation2071351;Name=-10
BBa_I50052	.	sequence_feature	737	756	.	+	0	ID=annotation2071345;Name=direct repeat
BBa_I50052	.	sequence_feature	1847	1861	.	+	0	ID=annotation2071356;Name=inverted repeat
BBa_I50052	.	sequence_alteration	1750	1750	.	+	0	ID=annotation2071360;Name=C to T difference with canonical pSC101 vector sequence
>BBa_I50052
ctgtcagaccaagtttacgagctcgcttggactcctgttgatagatccagtaatgacctcagaactccatctggatttg
ttcagaacgctcggttgccgccgggcgttttttattggtgagaatccaagcactagggacagtaagacgggtaagcctg
ttgatgataccgctgccttactgggtgcattagccagtctgaatgacctgtcacgggataatccgaagtggtcagactg
gaaaatcagagggcaggaactgctgaacagcaaaaagtcagatagcaccacatagcagacccgccataaaacgccctga
gaagcccgtgacgggcttttcttgtattatgggtagtttccttgcatgaatccataaaaggcgcctgtagtgccattta
cccccattcactgccagagccgtgagcgcagcgaactgaatgtcacgaaaaagacagcgactcaggtgcctgatggtcg
gagacaaaaggaatattcagcgatttgcccgagcttgcgagggtgctacttaagcctttagggttttaaggtctgtttt
gtagaggagcaaacagcgtttgcgacatccttttgtaatactgcggaactgactaaagtagtgagttatacacagggct
gggatctattctttttatctttttttattctttctttattctataaattataaccacttgaatataaacaaaaaaaaca
cacaaaggtctagcggaatttacagagggtctagcagaatttacaagttttccagcaaaggtctagcagaatttacaga
tacccacaactcaaaggaaaaggacatgtaattatcattgactagcccatctcaattggtatagtgattaaaatcacct
agaccaattgagatgtatgtctgaattagttgttttcaaagcaaatgaactagcgattagtcgctatgacttaacggag
catgaaaccaagctaattttatgctgtgtggcactactcaaccccacgattgaaaaccctacaaggaaagaacggacgg
tatcgttcacttataaccaatacgttcagatgatgaacatcagtagggaaaatgcttatggtgtattagctaaagcaac
cagagagctgatgacgagaactgtggaaatcaggaatcctttggttaaaggctttgagattttccagtggacaaactat
gccaagttctcaagcgaaaaattagaattagtttttagtgaagagatattgccttatcttttccagttaaaaaaattca
taaaatataatctggaacatgttaagtcttttgaaaacaaatactctatgaggatttatgagtggttattaaaagaact
aacacaaaagaaaactcacaaggcaaatatagagattagccttgatgaatttaagttcatgttaatgcttgaaaataac
taccatgagtttaaaaggcttaaccaatgggttttgaaaccaataagtaaagatttaaacacttacagcaatatgaaat
tggtggttgataagcgaggccgcccgactgatacgttgattttccaagttgaactagatagacaaatggatctcgtaac
cgaacttgagaacaaccagataaaaatgaatggtgacaaaataccaacaaccattacatcagattcctacctacataac
ggactaagaaaaacactacacgatgctttaactgcaaaaattcagctcaccagttttgaggcaaaatttttgagtgaca
tgcaaagtaagtatgatctcaatggttcgttctcatggctcacgcaaaaacaacgaaccacactagagaacatactggc
taaatacggaaggatctgaggttcttatggctcttgtatctatcagtgaagcatcaagactaacaaacaaaagtagaac
aactgttcaccgttacatatcaaagggaaaactgtccatatgcacagatgaaaacggtgtaaaaaagatagatacatca
gagcttttacgagtttttggtgcattcaaagctgttcaccatgaacagatcgacaatgtaac