##gff-version 3
##sequence-region BBa_I50030 108 1778
BBa_I50030	.	sequence_feature	171	759	.	+	0	ID=annotation1796194;Name=pMB1 origin of replication
BBa_I50030	.	sequence_feature	1126	1282	.	+	0	ID=annotation1796206;Name=H-strand Y effector site
BBa_I50030	.	sequence_feature	758	758	.	+	0	ID=annotation1796203;Name=replication origin
BBa_I50030	.	ribosome_entry_site	1384	1388	.	+	0	ID=annotation1796208;Name=RBS 2
BBa_I50030	.	sequence_feature	1501	1505	.	+	0	ID=annotation1796209;Name=gamma-delta insertion target sequence
BBa_I50030	.	sequence_feature	160	774	.	+	0	ID=annotation1796201;Name=rep pMB1
BBa_I50030	.	promoter	171	176	.	+	0	ID=annotation1796196;Name=RNAII transcript promoter -35 sequence
BBa_I50030	.	promoter	348	353	.	+	0	ID=annotation1796200;Name=RNAI transcript promoter -35 sequence
BBa_I50030	.	sequence_feature	1774	1778	.	+	0	ID=annotation1796211;Name=gamma-delta insertion target sequence
BBa_I50030	.	ribosome_entry_site	1383	1388	.	+	0	ID=annotation1796207;Name=RBS 1
BBa_I50030	.	promoter	192	197	.	+	0	ID=annotation1796197;Name=RNAII transcript promoter -10 sequence
BBa_I50030	.	sequence_feature	879	942	.	+	0	ID=annotation1796204;Name=L-strand Y effector site
BBa_I50030	.	sequence_feature	108	145	.	+	0	ID=annotation1796195;Name=repeat region found in Tn3
BBa_I50030	.	sequence_feature	207	759	.	+	0	ID=annotation1796202;Name=RNAII transcript
BBa_I50030	.	sequence_feature	1044	1048	.	+	0	ID=annotation1796205;Name=gamma-delta insertion target sequence
BBa_I50030	.	sequence_feature	210	317	.	+	0	ID=annotation1796198;Name=RNAI transcript
BBa_I50030	.	promoter	326	331	.	+	0	ID=annotation1796199;Name=RNAI transcript promoter -10 sequence
BBa_I50030	.	sequence_feature	1531	1657	.	+	0	ID=annotation1796210;Name=from pSC101
>BBa_I50030
ctgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaaga
tcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagat
caaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtg
gtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactg
tccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcct
gttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcg
cagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctac
agcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaac
aggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgactt
gagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcc
tggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctt
tgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcctg
atgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatggtgcactctcagtacaatctgctctgatg
ccgcatagttaagccagtatacactccgctatcgctacgtgactgggtcatggctgcgccccgacacccgccaacaccc
gctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgt
gtcagaggttttcaccgtcatcaccgaaacgcgcgaggcagctgcggtaaagctcatcagcgtggtcgtgaagcgattc
acagatgtctgcctgttcatccgcgtccagctcgttgagtttctccagaagcgttaatgtctggcttctgataaagcgg
gccatgttaagggcggttttttcctgtttggtcactgatgcctccgtgtaagggggatttctgttcatgggggtaatga
taccgatgaaacgagagaggatgctcacgatacgggttactgatgatgaacatgcccggttactggaacgttgtgaggg
taaacaactggcggtatggatgcggcgggaccagagaaaaatcactcagggtcaatgccagcgcttcgttaatacagat
gtaggtgttccacagggtagccagcagcatcctgcgatgcagatccggaacataatggtgcagggcgctgacttccgcg
tttccagactttacgaaacacggaaaccgaagaccattcatgttgttgctcaggtcgcagacgttttgcagcagcagtc
gcttcacgttcgctcgcgtatcggtgattcattctgctaaccagtaaggcaaccccgccagcctagccgggtcctcaac
gacaggagcacgatcatgcgcacccgtggccaggacccaacgctgcccgagatgcgccgcgtgcggctgctggagatgg
cggacgcgatggatatgttctgccaagggttggtttgcgcattcacagttctccgcaagaattgattggctccaattct
tggagtggtgaatccgttagcgaggtgccgccggcttccat