##gff-version 3
##sequence-region BBa_I50020 16 630
BBa_I50020	.	sequence_alteration	467	467	.	+	0	ID=annotation1910691;Name=C to T, nonfunctional mutation
BBa_I50020	.	sequence_alteration	261	261	.	+	0	ID=annotation1910687;Name=G to A, nonfunctional mutation
BBa_I50020	.	promoter	48	53	.	+	0	ID=annotation1793685;Name=RNAII transcript promoter -10 sequence
BBa_I50020	.	sequence_feature	16	630	.	+	0	ID=annotation1782180;Name=rep (pMB1)
BBa_I50020	.	sequence_alteration	265	265	.	+	0	ID=annotation1910688;Name=G to A, nonfunctional mutation
BBa_I50020	.	sequence_feature	615	615	.	+	0	ID=annotation1782181;Name=ORI
BBa_I50020	.	sequence_feature	66	173	.	+	0	ID=annotation1793684;Name=RNAI transcript
BBa_I50020	.	promoter	27	32	.	+	0	ID=annotation1793686;Name=RNAII transcript promoter -35 sequence
BBa_I50020	.	sequence_alteration	274	274	.	+	0	ID=annotation1910689;Name=G to A, nonfunctional mutation
BBa_I50020	.	promoter	204	209	.	+	0	ID=annotation1793682;Name=RNAI transcript promoter -35
BBa_I50020	.	promoter	182	187	.	+	0	ID=annotation1793683;Name=RNAI transcript promoter -10
BBa_I50020	.	sequence_feature	63	615	.	+	0	ID=annotation1793681;Name=RNAII transcript
BBa_I50020	.	sequence_feature	27	615	.	+	0	ID=annotation1793680;Name=origin of replication
BBa_I50020	.	sequence_alteration	303	303	.	+	0	ID=annotation1910690;Name=C to T, nonfunctional mutation
>BBa_I50020
cccgtagaaaagatcaaaagatcttcttgagatcctttttttctgcgcgtaatctgctacttgcaaacaaaaaaaccac
cgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggctttagcagagcgca
gataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctc
gctctgctaatcctgttaccagtagctactgccagtagcgataagtcgtgtcttaccgggttggattcaagacgatagt
taccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccga
actgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatctggtaagc
ggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttc
gccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggc
ctttttacggttcctggccttttgctggccttttgctcacat