##gff-version 3
##sequence-region BBa_I50000 1 4640
BBa_I50000	.	sequence_feature	1	1390	.	+	0	ID=annotation1757407;Name=sequence derived from F plasmid in GenBank Accession Number AP001918
BBa_I50000	.	CDS	2893	3864	.	+	0	ID=annotation1757405;Name=parB
BBa_I50000	.	point_mutation	2553	2553	.	+	0	ID=annotation1757410;Name=C to T mutation (eliminates PstI site)
BBa_I50000	.	sequence_feature	268	287	.	+	0	ID=annotation1757419;Name=iteron (repeat region)
BBa_I50000	.	sequence_feature	101	147	.	+	0	ID=annotation1757415;Name=A/T region
BBa_I50000	.	sequence_feature	192	211	.	+	0	ID=annotation1757416;Name=iteron (repeat region)
BBa_I50000	.	sequence_feature	1720	4640	.	+	0	ID=annotation1757421;Name=sequence derived from F plasmid in GenBank Accession Number AP001918
BBa_I50000	.	CDS	384	1139	.	+	0	ID=annotation1757408;Name=repE
BBa_I50000	.	sequence_feature	3938	4640	.	+	0	ID=annotation1757395;Name=sopC(IncD) repeat region
BBa_I50000	.	point_mutation	1013	1013	.	+	0	ID=annotation1757412;Name=C to T mutation (eliminates PstI site)
BBa_I50000	.	sequence_feature	1	290	.	+	0	ID=annotation1757409;Name=ori2 (oriS) single copy replication origin
BBa_I50000	.	operator	81	88	.	+	0	ID=annotation1757420;Name=DnaA box
BBa_I50000	.	operator	89	97	.	+	0	ID=annotation1757414;Name=DnaA box
BBa_I50000	.	point_mutation	3711	3711	.	+	0	ID=annotation1757411;Name=A to T mutation (eliminates SpeI site)
BBa_I50000	.	sequence_feature	246	265	.	+	0	ID=annotation1757418;Name=iteron (repeat region)
BBa_I50000	.	sequence_feature	213	232	.	+	0	ID=annotation1757417;Name=iteron (repeat region)
BBa_I50000	.	CDS	1718	2893	.	+	0	ID=annotation1757406;Name=parA
BBa_I50000	.	sequence_alteration	183	183	.	+	0	ID=annotation1757413;Name=C to G mutation (eliminates XbaI site, noncoding region)
>BBa_I50000
tcgagtgagcgaggaagcaccagggaacagcacttatatattctgcttacacacgatgcctgaaaaaacttcccttggg
gttatccacttatccacggggatatttttataattattttttttatagtttttagatcttcttttttagagcgccttgt
aggcctttatccatgctggttctacagaaggtgttgtgacaaattgccctttcagtgtgacaaatcaccctcaaatgac
agtcctgtctgtgacaaattgcccttaaccctgtgacaaattgccctcagaagaagctgttttttcacaaagttatccc
tgcttattgactcttttttatttagtgtgacaatctaaaaacttgtcacacttcacatggatctgtcatggcggaaaca
gcggttatcaatcacaagaaacgtaaaaatagcccgcgaatcgtccagtcaaacgacctcactgaggcggcatatagtc
tctcccgggatcaaaaacgtatgctgtatctgttcgttgaccagatcagaaaatctgatggcaccctacaggaacatga
cggtatctgcgagatccatgttgctaaatatgctgaaatattcggattgacctctgcggaagccagtaaggatatacgg
caggcattgaagagtttcgcggggaaggaagtggttttttatcgccctgaagaggatgccggcgatgaaaaaggctatg
aatcttttccttggtttatcaaacgtgcgcacagtccatccagagggctttacagtgtacatatcaacccatatctcat
tcccttctttatcgggttacagaaccggtttacgcagtttcggcttagtgaaacaaaagaaatcaccaatccgtatgcc
atgcgtttatacgaatccctgtgtcagtatcgtaagccggatggctcaggcatcgtctctctgaaaatcgactggatca
tagagcgttaccagctgcctcaaagttaccagcgtatgcctgacttccgccgccgcttcctgcaagtctgtgttaatga
gatcaacagcagaactccaatgcgcctctcatacattgagaaaaagaaaggccgccagacgactcatatcgtattttcc
ttccgcgatatcacttccatgacgacaggatagtctgagggttatctgtcacagatttgagggtggttcgtcacatttg
ttctgacctactgagggtaatttgtcacagttttgctgtttccttcagcctgcatggattttctcatactttttgaact
gtaatttttaaggaagccaaatttgagggcagtttgtcacagttgatttccttctctttcccttcgtcatgtgacttga
tatcgggggttagttcgtcatcattgatgagggttgattatcacagtttattactctgaattggctatccgcgtgtgta
cctctacctggagtttttcccacggtggatatttcttcttgcgctgagcgtaagagctatctgacagaacagttcttct
ttgcttcctcgccagttcgctcgctatgctcggttacacggctgcggcgagcgctagtgataataagtgactgaggtat
gtgctcttcttatctccttttgtagtgttgctcttattttaaacaactttgcggttttttgatgactttgcgattttgt
tgttgctttgcagtaaattgcaagatttaataaaaaaacgcaaagcaatgattaaaggatgttcagaatgaaactcatg
gaaacacttaaccagtgcataaacgctggtcatgaaatgacgaaggctatcgccattgcacagtttaatgatgacagcc
cggaagcgaggaaaataacccggcgctggagaataggtgaagcagcggatttagttggggtttcttctcaggctatcag
agatgccgagaaagcagggcgactaccgcacccggatatggaaattcgaggacgggttgagcaacgtgttggttataca
attgaacaaattaatcatatgcgtgatgtgtttggtacgcgattgcgacgtgctgaagacgtatttccaccggtgatcg
gggttgctgcccataaaggtggcgtttacaaaacctcagtttctgttcatcttgctcaggatctggctctgaaggggct
acgtgttttgctcgtggaaggtaacgacccccagggaacagcctcaatgtatcacggatgggtaccagatcttcatatt
catgcagaagacactctcctgcctttctatcttggggaaaaggacgatgtcacttatgcaataaagcccacttgctggc
cggggcttgacattattccttcctgtctggctctgcaccgtattgaaactgagttaatgggcaaatttgatgaaggtaa
actgcccaccgatccacacctgatgctccgactggccattgaaactgttgctcatgactatgatgtcatagttattgac
agcgcgcctaacctgggtatcggcacgattaatgtcgtatgtgctgctgatgtgctgattgttcccacgcctgctgagt
tgtttgactacacctccgcactgcaatttttcgatatgcttcgtgatctgctcaagaacgttgatcttaaagggttcga
gcctgatgtacgtattttgcttaccaaatacagcaatagtaatggctctcagtccccgtggatggaggagcaaattcgg
gatgcctggggaagcatggttctaaaaaatgttgtacgtgaaacggatgaagttggtaaaggtcagatccggatgagaa
ctgtttttgaacaggccattgatcaacgctcttcaactggtgcctggagaaatgctctttctatttgggaacctgtctg
caatgaaattttcgatcgtctgattaaaccacgctgggagattagataatgaagcgtgcgcctgttattccaaaacata
cgctcaatactcaaccggttgaagatacttcgttatcgacaccagctgccccgatggtggattcgttaattgcgcgcgt
aggagtaatggctcgcggtaatgccattactttgcctgtatgtggtcgggatgtgaagtttactcttgaagtgctccgg
ggtgatagtgttgagaagacctctcgggtatggtcaggtaatgaacgtgaccaggagctgcttactgaggacgcactgg
atgatctcatcccttcttttctactgactggtcaacagacaccggcgttcggtcgaagagtatctggtgtcatagaaat
tgccgatgggagtcgccgtcgtaaagctgctgcacttaccgaaagtgattatcgtgttctggttggcgagctggatgat
gagcagatggctgcattatccagattgggtaacgattatcgcccaacaagtgcttatgaacgtggtcagcgttatgcaa
gccgattgcagaatgaatttgctggaaatatttctgcgctggctgatgcggaaaatatttcacgtaagattattacccg
ctgtatcaacaccgccaaattgcctaaatcagttgttgctcttttttctcaccccggtgaactatctgcccggtcaggt
gatgcacttcaaaaagcctttacagataaagaggaattacttaagcagcaggcatctaaccttcatgagcagaaaaaag
ctggggtgatatttgaagctgaagaagttatcactcttttaacttctgtgcttaaaacgtcatctgcatcaagaacaag
tttaagctcacgacatcagtttgctcctggagcgacagtattgtataagggcgataaaatggtgcttaacctggacagg
tctcgtgttccaactgagtgtatagagaaaattgaggccattcttaaggaacttgaaaagccagcaccctgatgcgacc
acgttttagtctacgtttatctgtctttacttaatgtcctttgttacaggccagaaagcataactggcctgaatattct
ctctgggcccactgttccacttgtatcgtcggtctgataatcagactgggaccacggtcccactcgtatcgtcggtctg
attattagtctgggaccacggtcccactcgtatcgtcggtctgattattagtctgggaccacggtcccactcgtatcgt
cggtctgataatcagactgggaccacggtcccactcgtatcgtcggtctgattattagtctgggaccatggtcccactc
gtatcgtcggtctgattattagtctgggaccacggtcccactcgtatcgtcggtctgattattagtctggaaccacggt
cccactcgtatcgtcggtctgattattagtctgggaccacggtcccactcgtatcgtcggtctgattattagtctggga
ccacgatcccactcgtgttgtcggtctgattatcggtctgggaccacggtcccacttgtattgtcgatcagactatcag
cgtgagactacgattccatcaatgcctgtcaagggcaagtattgacatgtcgtcgtaacctgtagaacggagtaacctc
ggtgtgcggttgtatgcctgctgtggattgctgctgtgtcctgcttatccacaacattttgcgcacggttatgtggaca
aaatacctggttacccaggccgtgccggcacgttcaccgggctgcatccgatgcaagt