##gff-version 3
##sequence-region BBa_I4003 1 8612
BBa_I4003	.	CDS	5682	6470	.	+	0	ID=BBa_C0060;Name=BBa_C0060
BBa_I4003	.	engineered_tag	6471	6495	.	+	0	ID=annotation2213987;Name=Help:Barcodes;Parent=BBa_C0060
BBa_I4003	.	CDS	5682	6431	.	+	0	ID=annotation1757;Name=aiiA;Parent=BBa_C0060
BBa_I4003	.	tag	6432	6464	.	+	0	ID=annotation1756;Name=LVA;Parent=BBa_C0060
BBa_I4003	.	stop_codon	6465	6470	.	+	0	ID=annotation1755;Name=2;Parent=BBa_C0060
BBa_I4003	.	engineered_region	5682	6470	.	+	0	ID=annotation7037;Name=BBa_C0060;Parent=BBa_C0060
BBa_I4003	.	start_codon	5682	5684	.	+	0	ID=annotation1754;Name=start;Parent=BBa_C0060
BBa_I4003	.	terminator	770	849	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I4003	.	engineered_region	770	849	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I4003	.	stem_loop	781	824	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I4003	.	promoter	3433	3583	.	+	0	ID=BBa_R0063;Name=BBa_R0063
BBa_I4003	.	engineered_region	3433	3583	.	+	0	ID=annotation7071;Name=BBa_R0063;Parent=BBa_R0063
BBa_I4003	.	start_codon	3560	3560	.	+	0	ID=annotation2054;Name=start;Parent=BBa_R0063
BBa_I4003	.	promoter	3547	3554	.	+	0	ID=annotation2052;Name=-10;Parent=BBa_R0063
BBa_I4003	.	non_covalent_binding_site	3433	3452	.	+	0	ID=annotation2051;Name=LuxR/HSL;Parent=BBa_R0063
BBa_I4003	.	non_covalent_binding_site	3562	3581	.	+	0	ID=annotation2055;Name=Putative LuxR/HSL;Parent=BBa_R0063
BBa_I4003	.	promoter	3521	3526	.	+	0	ID=annotation2053;Name=-35;Parent=BBa_R0063
BBa_I4003	.	ribosome_entry_site	6703	6717	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_I4003	.	engineered_region	6703	6717	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_I4003	.	ribosome_entry_site	6703	6717	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_I4003	.	ribosome_entry_site	6710	6714	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_I4003	.	ribosome_entry_site	7693	7707	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_I4003	.	engineered_region	7693	7707	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_I4003	.	ribosome_entry_site	7693	7707	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_I4003	.	ribosome_entry_site	7700	7704	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_I4003	.	promoter	7631	7684	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I4003	.	promoter	7673	7678	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I4003	.	promoter	7650	7655	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I4003	.	engineered_region	7631	7684	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I4003	.	non_covalent_binding_site	7656	7674	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I4003	.	non_covalent_binding_site	7631	7649	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I4003	.	terminator	8484	8563	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I4003	.	engineered_region	8484	8563	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I4003	.	stem_loop	8495	8538	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I4003	.	CDS	2513	3262	.	+	0	ID=BBa_C0051;Name=BBa_C0051
BBa_I4003	.	engineered_tag	3263	3287	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_I4003	.	CDS	2516	3223	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_I4003	.	tag	3224	3256	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_I4003	.	terminator	2149	2228	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I4003	.	engineered_region	2149	2228	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I4003	.	stem_loop	2160	2203	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I4003	.	promoter	1	49	.	+	0	ID=BBa_R0051;Name=BBa_R0051
BBa_I4003	.	promoter	38	43	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_I4003	.	non_covalent_binding_site	25	41	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_I4003	.	engineered_region	1	49	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_I4003	.	non_covalent_binding_site	1	17	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_I4003	.	promoter	15	20	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_I4003	.	promoter	2286	2485	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_I4003	.	non_covalent_binding_site	2374	2411	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_I4003	.	promoter	2446	2451	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_I4003	.	engineered_region	2286	2485	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_I4003	.	CDS	2286	2373	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_I4003	.	non_covalent_binding_site	2451	2485	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_I4003	.	promoter	2422	2427	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_I4003	.	start_codon	2458	2458	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_I4003	.	terminator	6592	6632	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I4003	.	engineered_region	6592	6632	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I4003	.	stem_loop	6599	6618	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I4003	.	polyA_site	6619	6632	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I4003	.	stop_codon	6625	6625	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I4003	.	promoter	907	960	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I4003	.	promoter	949	954	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I4003	.	promoter	926	931	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I4003	.	engineered_region	907	960	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I4003	.	non_covalent_binding_site	932	950	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I4003	.	non_covalent_binding_site	907	925	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I4003	.	terminator	5316	5395	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I4003	.	engineered_region	5316	5395	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I4003	.	stem_loop	5327	5370	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I4003	.	terminator	7582	7622	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I4003	.	engineered_region	7582	7622	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I4003	.	stem_loop	7589	7608	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I4003	.	polyA_site	7609	7622	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I4003	.	stop_codon	7615	7615	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I4003	.	ribosome_entry_site	58	70	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_I4003	.	engineered_region	58	70	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_I4003	.	ribosome_entry_site	64	67	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_I4003	.	ribosome_entry_site	58	70	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_I4003	.	terminator	8572	8612	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I4003	.	engineered_region	8572	8612	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I4003	.	stem_loop	8579	8598	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I4003	.	polyA_site	8599	8612	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I4003	.	stop_codon	8605	8605	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I4003	.	terminator	7494	7573	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I4003	.	engineered_region	7494	7573	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I4003	.	stem_loop	7505	7548	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I4003	.	ribosome_entry_site	4644	4658	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_I4003	.	engineered_region	4644	4658	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_I4003	.	ribosome_entry_site	4644	4658	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_I4003	.	ribosome_entry_site	4651	4655	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_I4003	.	engineered_region	7714	8475	.	+	0	ID=BBa_E0032;Name=BBa_E0032
BBa_I4003	.	engineered_region	7714	8475	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_I4003	.	stop_codon	8470	8475	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_I4003	.	CDS	7714	8475	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_I4003	.	sequence_alteration	7955	7955	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_I4003	.	tag	8431	8469	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_I4003	.	terminator	6504	6583	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I4003	.	engineered_region	6504	6583	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I4003	.	stem_loop	6515	6558	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I4003	.	ribosome_entry_site	5661	5675	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_I4003	.	engineered_region	5661	5675	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_I4003	.	ribosome_entry_site	5661	5675	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_I4003	.	ribosome_entry_site	5668	5672	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_I4003	.	CDS	988	2115	.	+	0	ID=BBa_C0012;Name=BBa_C0012
BBa_I4003	.	engineered_region	988	2115	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_I4003	.	engineered_tag	2116	2140	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_I4003	.	tag	2077	2115	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_I4003	.	CDS	988	2115	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_I4003	.	promoter	5453	5652	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_I4003	.	non_covalent_binding_site	5541	5578	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_I4003	.	promoter	5613	5618	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_I4003	.	engineered_region	5453	5652	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_I4003	.	CDS	5453	5540	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_I4003	.	non_covalent_binding_site	5618	5652	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_I4003	.	promoter	5589	5594	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_I4003	.	start_codon	5625	5625	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_I4003	.	engineered_region	6724	7485	.	+	0	ID=BBa_E0022;Name=BBa_E0022
BBa_I4003	.	CDS	6724	7485	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_I4003	.	tag	7442	7479	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
BBa_I4003	.	engineered_region	6724	7485	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
BBa_I4003	.	point_mutation	6792	6792	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_I4003	.	stop_codon	7480	7485	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_I4003	.	terminator	4490	4530	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I4003	.	engineered_region	4490	4530	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I4003	.	stem_loop	4497	4516	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I4003	.	polyA_site	4517	4530	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I4003	.	stop_codon	4523	4523	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I4003	.	CDS	3613	4368	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_I4003	.	engineered_region	3613	3614	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_I4003	.	engineered_region	3613	4368	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_I4003	.	engineered_tag	4369	4393	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_I4003	.	point_mutation	4104	4104	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_I4003	.	point_mutation	3786	3786	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_I4003	.	CDS	3613	4362	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_I4003	.	ribosome_entry_site	3592	3606	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_I4003	.	engineered_region	3592	3606	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_I4003	.	ribosome_entry_site	3592	3606	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_I4003	.	ribosome_entry_site	3599	3603	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_I4003	.	terminator	3296	3375	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I4003	.	engineered_region	3296	3375	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I4003	.	stem_loop	3307	3350	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I4003	.	terminator	3384	3424	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I4003	.	engineered_region	3384	3424	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I4003	.	stem_loop	3391	3410	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I4003	.	polyA_site	3411	3424	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I4003	.	stop_codon	3417	3417	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I4003	.	CDS	77	736	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_I4003	.	CDS	80	696	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_I4003	.	engineered_tag	737	761	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_I4003	.	tag	697	730	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_I4003	.	CDS	4665	5282	.	+	0	ID=BBa_C0061;Name=BBa_C0061
BBa_I4003	.	CDS	4665	5243	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_I4003	.	tag	5244	5275	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_I4003	.	engineered_region	4665	5282	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_I4003	.	engineered_tag	5283	5307	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_I4003	.	terminator	4402	4481	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I4003	.	engineered_region	4402	4481	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I4003	.	stem_loop	4413	4456	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I4003	.	ribosome_entry_site	969	981	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_I4003	.	engineered_region	969	981	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_I4003	.	ribosome_entry_site	975	978	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_I4003	.	ribosome_entry_site	969	981	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_I4003	.	terminator	2237	2277	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I4003	.	engineered_region	2237	2277	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I4003	.	stem_loop	2244	2263	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I4003	.	polyA_site	2264	2277	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I4003	.	stop_codon	2270	2270	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I4003	.	promoter	4539	4635	.	+	0	ID=BBa_R0065;Name=BBa_R0065
BBa_I4003	.	promoter	4609	4614	.	+	0	ID=annotation1986779;Name=-35;Parent=BBa_R0065
BBa_I4003	.	engineered_region	4539	4635	.	+	0	ID=annotation1986774;Name=BBa_R0065;Parent=BBa_R0065
BBa_I4003	.	promoter	4596	4596	.	+	0	ID=annotation1986778;Name=lux p(R) start;Parent=BBa_R0065
BBa_I4003	.	non_covalent_binding_site	4595	4611	.	+	0	ID=annotation1986777;Name=OR2 cI;Parent=BBa_R0065
BBa_I4003	.	promoter	4632	4635	.	+	0	ID=annotation1986781;Name=-10;Parent=BBa_R0065
BBa_I4003	.	non_covalent_binding_site	4619	4635	.	+	0	ID=annotation1986780;Name=OR1 cI;Parent=BBa_R0065
BBa_I4003	.	non_covalent_binding_site	4544	4563	.	+	0	ID=annotation1986775;Name=Lux Box;Parent=BBa_R0065
BBa_I4003	.	promoter	4585	4590	.	+	0	ID=annotation1986776;Name=-10;Parent=BBa_R0065
BBa_I4003	.	terminator	858	898	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I4003	.	engineered_region	858	898	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I4003	.	stem_loop	865	884	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I4003	.	polyA_site	885	898	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I4003	.	stop_codon	891	891	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I4003	.	ribosome_entry_site	2494	2506	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_I4003	.	engineered_region	2494	2506	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_I4003	.	ribosome_entry_site	2500	2503	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_I4003	.	ribosome_entry_site	2494	2506	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_I4003	.	terminator	5404	5444	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I4003	.	engineered_region	5404	5444	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I4003	.	stem_loop	5411	5430	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I4003	.	polyA_site	5431	5444	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I4003	.	stop_codon	5437	5437	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I4003	.	promoter	6641	6694	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I4003	.	promoter	6683	6688	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I4003	.	promoter	6660	6665	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I4003	.	engineered_region	6641	6694	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I4003	.	non_covalent_binding_site	6666	6684	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I4003	.	non_covalent_binding_site	6641	6659	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
>BBa_I4003
taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagtcacacaggaaagtactagatg
tccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaaccc
gtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgc
cttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgt
aataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacag
aaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgc
actcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaa
acacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagcct
tcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacga
aaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaa
aggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctc
accttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgataga
gatactgagcactactagagtcacacaggaaagtactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcag
agtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaa
agtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctg
attggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaac
tgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgc
gcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaat
gttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgac
tgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcg
tctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagt
gccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatc
agatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacga
cgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagc
gtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaa
aaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggt
ttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgt
agatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgttt
gtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatac
gcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcag
tgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatg
ttgtgtggaattgtgagcggataacaatttcacacatactagagtcacacaggaaagtactagatgagcacaaaaaaga
aaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggctt
atcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcatta
aatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaa
tctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttca
ggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagcc
agtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacg
gaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtt
taccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgc
aatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaa
actacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaag
gctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcac
cttcgggtgggcctttctgcgtttatatactagagacctgtacgatcctacaggtgcttatgttaagtaattgtattcc
cagcgatacaatagtgtgacaaaaatccaatttattagaatcaaatgtcaatccattaccgttttaatgatatataaca
cgcaaaacttgcgacaaacaataggtaatactagagattaaagaggagaaatactagatgaaaaacataaatgccgacg
acacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaa
aatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagat
aattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaact
ccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagc
gaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgca
cattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctag
ttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagc
gtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccattta
accaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgatt
gcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaag
gctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcac
cttcgggtgggcctttctgcgtttatatactagagtaagcacctgtaggatcgtacaggtttacgcaagaaaatggttt
gttatagtcgaataacaccgtgcgtgttgactattttacctctggcggtgatatactagagattaaagaggagaaatac
tagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgtt
atcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaa
tgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattat
atgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgtt
ttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataa
acacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaa
gttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctatta
atgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtg
ctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatct
gttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactaga
gcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaag
cgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggc
tcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagattaaagaggagaaatactagatgacag
taaagaagctttatttcgtcccagcaggtcgttgtatgttggatcattcgtctgttaatagtacattaacaccaggaga
attattagacttaccggtttggtgttatcttttggagactgaagaaggacctattttagtagatacaggtatgccagaa
agtgcagttaataatgaaggtctttttaacggtacatttgtcgaagggcaggttttaccgaaaatgactgaagaagata
gaatcgtgaatattttaaaacgggttggttatgagccggaagaccttctttatattattagttctcacttgcattttga
tcatgcaggaggaaatggcgcttttataaatacaccaatcattgtacagcgtgctgaatatgaggcggcgcagcatagc
gaagaatatttgaaagaatgtatattgccgaatttaaactacaaaatcattgaaggtgattatgaagtcgtaccaggag
ttcaattattgcatacaccaggccatactccagggcatcaatcgctattaattgagacagaaaaatccggtcctgtatt
attaacgattgatgcatcgtatacgaaagagaattttgaaaatgaagtgccatttgcgggatttgattcagaattagct
ttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaagccgattgttttctttggacatgatatagagcagg
aaaggggatgtaaagtgttccctgaatatatagctgcaaacgacgaaaactacgctttagtagcttaataacgctgata
gtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttta
tctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatact
agagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagattaaagaggaga
aatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaa
cggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcacc
accggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccg
accacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaagga
cgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatc
gacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccg
acaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgacca
ctaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctg
agcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatgg
acgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaa
taaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtca
cactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatc
agtgatagagatactgagcactactagagattaaagaggagaaatactagatggtgagcaagggcgaggagctgttcac
cggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggc
gatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtga
ccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccat
gcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttc
gagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagc
tggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagat
ccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtg
ctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcc
tgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaa
ctacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcg
ttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
a