##gff-version 3
##sequence-region BBa_I3522 1 3505
BBa_I3522	.	engineered_region	983	2636	.	+	0	ID=BBa_I3520;Name=BBa_I3520
BBa_I3522	.	engineered_region	2147	2499	.	+	0	ID=BBa_E0033;Name=BBa_E0033;Parent=BBa_I3520
BBa_I3522	.	sequence_alteration	2254	2254	.	+	0	ID=annotation308383;Name=C;Parent=BBa_E0033
BBa_I3522	.	sequence_feature	2147	2161	.	+	0	ID=annotation1938942;Name=lacZ gene fragment;Parent=BBa_E0033
BBa_I3522	.	promoter	2172	2181	.	+	0	ID=annotation1938939;Name=T3 promoter;Parent=BBa_E0033
BBa_I3522	.	sequence_alteration	2227	2227	.	+	0	ID=annotation308375;Name=T;Parent=BBa_E0033
BBa_I3522	.	sequence_alteration	2257	2257	.	+	0	ID=annotation308388;Name=G;Parent=BBa_E0033
BBa_I3522	.	sequence_feature	2345	2494	.	+	0	ID=annotation1938941;Name=lacZ gene fragment;Parent=BBa_E0033
BBa_I3522	.	sequence_alteration	2233	2233	.	+	0	ID=annotation308381;Name=A;Parent=BBa_E0033
BBa_I3522	.	promoter	2319	2337	.	+	0	ID=annotation1938940;Name=T7 promoter;Parent=BBa_E0033
BBa_I3522	.	CDS	2147	2494	.	+	0	ID=annotation308320;Name=lacZ alpha;Parent=BBa_E0033
BBa_I3522	.	sequence_alteration	2224	2224	.	+	0	ID=annotation308387;Name=C;Parent=BBa_E0033
BBa_I3522	.	terminator	2508	2636	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_I3520
BBa_I3522	.	terminator	2508	2587	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I3522	.	stem_loop	2519	2562	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I3522	.	engineered_region	2508	2587	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I3522	.	terminator	2596	2636	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I3522	.	stop_codon	2629	2629	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I3522	.	polyA_site	2623	2636	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I3522	.	stem_loop	2603	2622	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I3522	.	engineered_region	2596	2636	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I3522	.	engineered_region	983	2140	.	+	0	ID=BBa_I3510;Name=BBa_I3510;Parent=BBa_I3520
BBa_I3522	.	CDS	1160	1915	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I3510
BBa_I3522	.	engineered_region	1160	1915	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_I3522	.	engineered_region	1160	1161	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_I3522	.	engineered_tag	1916	1940	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_I3522	.	point_mutation	1651	1651	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_I3522	.	point_mutation	1333	1333	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_I3522	.	CDS	1160	1909	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_I3522	.	ribosome_entry_site	1142	1153	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I3510
BBa_I3522	.	conserved_region	1146	1149	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I3522	.	promoter	983	1133	.	+	0	ID=BBa_R0063;Name=BBa_R0063;Parent=BBa_I3510
BBa_I3522	.	promoter	1071	1076	.	+	0	ID=annotation2053;Name=-35;Parent=BBa_R0063
BBa_I3522	.	promoter	1097	1104	.	+	0	ID=annotation2052;Name=-10;Parent=BBa_R0063
BBa_I3522	.	engineered_region	983	1133	.	+	0	ID=annotation7071;Name=BBa_R0063;Parent=BBa_R0063
BBa_I3522	.	start_codon	1110	1110	.	+	0	ID=annotation2054;Name=start;Parent=BBa_R0063
BBa_I3522	.	non_covalent_binding_site	1112	1131	.	+	0	ID=annotation2055;Name=Putative LuxR/HSL;Parent=BBa_R0063
BBa_I3522	.	non_covalent_binding_site	983	1002	.	+	0	ID=annotation2051;Name=LuxR/HSL;Parent=BBa_R0063
BBa_I3522	.	terminator	1949	2028	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I3510
BBa_I3522	.	stem_loop	1960	2003	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I3522	.	engineered_region	1949	2028	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I3522	.	promoter	2086	2140	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_I3510
BBa_I3522	.	start_codon	2138	2138	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_I3522	.	promoter	2105	2110	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_I3522	.	engineered_region	2086	2140	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_I3522	.	promoter	2127	2132	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_I3522	.	non_covalent_binding_site	2086	2105	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_I3522	.	terminator	2037	2077	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I3510
BBa_I3522	.	stop_codon	2070	2070	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I3522	.	polyA_site	2064	2077	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I3522	.	stem_loop	2044	2063	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I3522	.	engineered_region	2037	2077	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I3522	.	engineered_region	2645	3505	.	+	0	ID=BBa_I3500;Name=BBa_I3500
BBa_I3522	.	terminator	3377	3456	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I3500
BBa_I3522	.	stem_loop	3388	3431	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I3522	.	engineered_region	3377	3456	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I3522	.	promoter	2645	2699	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_I3500
BBa_I3522	.	start_codon	2697	2697	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_I3522	.	promoter	2664	2669	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_I3522	.	engineered_region	2645	2699	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_I3522	.	promoter	2686	2691	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_I3522	.	non_covalent_binding_site	2645	2664	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_I3522	.	terminator	3465	3505	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I3500
BBa_I3522	.	stop_codon	3498	3498	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I3522	.	polyA_site	3492	3505	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I3522	.	stem_loop	3472	3491	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I3522	.	engineered_region	3465	3505	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I3522	.	ribosome_entry_site	2708	2719	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I3500
BBa_I3522	.	conserved_region	2712	2715	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I3522	.	CDS	2726	3343	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_I3500
BBa_I3522	.	engineered_region	2726	3343	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_I3522	.	tag	3305	3336	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_I3522	.	CDS	2726	3304	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_I3522	.	engineered_tag	3344	3368	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_I3522	.	engineered_region	1	974	.	+	0	ID=BBa_I3601;Name=BBa_I3601
BBa_I3522	.	promoter	1	49	.	+	0	ID=BBa_R1075;Name=BBa_R1075;Parent=BBa_I3601
BBa_I3522	.	promoter	35	40	.	+	0	ID=annotation309020;Name=-10;Parent=BBa_R1075
BBa_I3522	.	promoter	15	20	.	+	0	ID=annotation309024;Name=-35;Parent=BBa_R1075
BBa_I3522	.	engineered_region	76	837	.	+	0	ID=BBa_E0032;Name=BBa_E0032;Parent=BBa_I3601
BBa_I3522	.	stop_codon	832	837	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_I3522	.	sequence_alteration	317	317	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_I3522	.	CDS	76	837	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_I3522	.	engineered_region	76	837	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_I3522	.	tag	793	831	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_I3522	.	terminator	846	925	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I3601
BBa_I3522	.	stem_loop	857	900	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I3522	.	engineered_region	846	925	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I3522	.	terminator	934	974	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I3601
BBa_I3522	.	stop_codon	967	967	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I3522	.	polyA_site	961	974	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I3522	.	stem_loop	941	960	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I3522	.	engineered_region	934	974	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I3522	.	ribosome_entry_site	58	69	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I3601
BBa_I3522	.	conserved_region	62	65	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_I3522
ttaaatttcctcttttcaggccggaataactccctataatgcgccaccatactagagaaagaggagaaatactagatgg
tgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagtt
cagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctg
cccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagc
tgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaacta
caagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggag
gacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaaga
acggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaa
cacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagacccc
aacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtaca
agaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaagg
ctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacc
ttcgggtgggcctttctgcgtttatatactagagacctgtacgatcctacaggtgcttatgttaagtaattgtattccc
agcgatacaatagtgtgacaaaaatccaatttattagaatcaaatgtcaatccattaccgttttaatgatatataacac
gcaaaacttgcgacaaacaataggtaatactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacac
atacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatg
gtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataatt
accctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaa
tcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaa
acatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacatt
cagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttga
taattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgg
gcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaacca
atgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgccc
atactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctc
agtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttc
gggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtc
gaataaatactagatgaccatgattacgccaagcgcgcaattaaccctcactaaagggaacaaaagctggagctccacc
gcggtggcggcagcactagagctagtggatcccccgggctgtagaaattcgatatcaagcttatcgataccgtcgacct
cgagggggggcccggtacccaattcgccctatagtgagtcgtattacgcgcgctcactggccgtcgttttacaacgtcg
tgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaa
gaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaataataatactagagccaggcatcaaataaaacgaa
aggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctc
accttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgtt
atagtcgaataaatactagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattc
catcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtaga
aaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagt
ggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtg
ctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctag
tgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaaca
tcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattag
gtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacga
aaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaa
aggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctc
accttcgggtgggcctttctgcgtttata