##gff-version 3
##sequence-region BBa_I3312 1 2769
BBa_I3312	.	terminator	1688	1728	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I3312	.	polyA_site	1715	1728	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I3312	.	stop_codon	1721	1721	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I3312	.	engineered_region	1688	1728	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I3312	.	stem_loop	1695	1714	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I3312	.	terminator	2729	2769	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I3312	.	polyA_site	2756	2769	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I3312	.	stop_codon	2762	2762	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I3312	.	engineered_region	2729	2769	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I3312	.	stem_loop	2736	2755	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I3312	.	terminator	2641	2720	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I3312	.	engineered_region	2641	2720	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I3312	.	stem_loop	2652	2695	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I3312	.	terminator	1600	1679	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I3312	.	engineered_region	1600	1679	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I3312	.	stem_loop	1611	1654	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I3312	.	promoter	1	49	.	+	0	ID=BBa_R1075;Name=BBa_R1075
BBa_I3312	.	promoter	35	40	.	+	0	ID=annotation309020;Name=-10;Parent=BBa_R1075
BBa_I3312	.	promoter	15	20	.	+	0	ID=annotation309024;Name=-35;Parent=BBa_R1075
BBa_I3312	.	promoter	1737	1844	.	+	0	ID=BBa_R0084;Name=BBa_R0084
BBa_I3312	.	non_covalent_binding_site	1787	1804	.	+	0	ID=annotation301313;Name=F3 OmpR;Parent=BBa_R0084
BBa_I3312	.	non_covalent_binding_site	1767	1784	.	+	0	ID=annotation301309;Name=F2 OmpR;Parent=BBa_R0084
BBa_I3312	.	promoter	1824	1829	.	+	0	ID=annotation301318;Name=-10;Parent=BBa_R0084
BBa_I3312	.	non_covalent_binding_site	1747	1764	.	+	0	ID=annotation301308;Name=F1 OmpR;Parent=BBa_R0084
BBa_I3312	.	promoter	1809	1814	.	+	0	ID=annotation301317;Name=-35;Parent=BBa_R0084
BBa_I3312	.	ribosome_entry_site	1853	1864	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I3312	.	conserved_region	1857	1860	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I3312	.	ribosome_entry_site	58	69	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I3312	.	conserved_region	62	65	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I3312	.	engineered_region	1871	2632	.	+	0	ID=BBa_E0022;Name=BBa_E0022
BBa_I3312	.	stop_codon	2627	2632	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_I3312	.	CDS	1871	2632	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_I3312	.	point_mutation	1939	1939	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_I3312	.	engineered_region	1871	2632	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
BBa_I3312	.	tag	2589	2626	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
BBa_I3312	.	CDS	76	1566	.	+	0	ID=BBa_C0082;Name=BBa_C0082
BBa_I3312	.	engineered_tag	1567	1591	.	+	0	ID=annotation2213997;Name=Help:Barcodes;Parent=BBa_C0082
BBa_I3312	.	CDS	76	840	.	+	0	ID=annotation308352;Name=Tar (front);Parent=BBa_C0082
BBa_I3312	.	point_mutation	498	499	.	+	0	ID=annotation300943;Name=A;Parent=BBa_C0082
BBa_I3312	.	CDS	847	1527	.	+	0	ID=annotation308360;Name=Envz (back);Parent=BBa_C0082
BBa_I3312	.	tag	1528	1560	.	+	0	ID=annotation301146;Name=LVA;Parent=BBa_C0082
>BBa_I3312
ttaaatttcctcttttcaggccggaataactccctataatgcgccaccatactagagaaagaggagaaatactagatga
ttaaccgtatccgcgtagtcacgctgttggtaatggtgctgggggtattcgcactgttacagcttatttccggcagtct
gtttttttcttcccttcaccatagccagaagagctttgtggtttccaatcaattacgggaacagcagggcgagctgacg
tcaacctgggatttaatgctgcaaacgcgcattaacctgagtcgttcagcggtacggatgatgatggattcctccaatc
aacaaagtaacgccaaagttgaattgctcgatagcgccaggaaaacattggcgcaggcagcgacgcattataaaaaatt
caaaagcatggcaccgttacctgaaatggtcgctaccagtcgtaatattgatgaaaaatataaaaactattacacagcg
ttaactgaactgattgattatctcgattatggcaatactggagcttatttcgctcagccaacccagggaatgcaaaatg
caatgggcgaagcgtttgctcagtacgccctcagcagtgaaaaactgtatcgcgatatcgtcactgacaacgcagatga
ttaccgatttgcccagtggcaactggcggttatcgcgctggtggtggtattgattctgctggtggcgtggtacggcatt
cgccgtatgttgcttactccgctggcaaaaattattgctcacattcgcgaaatcgccggtggtaacctggcgaataccc
tgaccattgacgggcgcagtgaaatgggcgacctggcgcagagcgtttcacatatggcggctggtgttaagcaactggc
ggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgactgag
atgatgagcgagcaggatggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgccatcattgagcagt
ttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtgattgc
tgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcg
atcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatggcaatggctggatcaaagtcagcagcggaacgg
agccgaatcgcgcctggttccaggtggaagatgacggtccgggaattgcgccggaacaacgtaagcacctgttccagcc
gtttgtccgcggcgacagtgcgcgcaccattagcggcacgggattagggctggcaattgtgcagcgtatcgtggataac
cataacgggatgctggagcttggcaccagcgagcggggcgggctttccattcgcgcctggctgccagtgccggtaacgc
gggcgcagggcacgacaaaagaaggggctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgcta
gtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgtt
gtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagct
taaattttacttttggttacatattttttctttttgaaaccaaatctttatctttgtagcactttcacggtagcgaaac
gttagtttgaatggaaagatgcctgcatactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgtt
caccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgag
ggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcg
tgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgc
catgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaag
ttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcaca
agctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaa
gatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggcccc
gtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatgg
tcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacga
aaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctt
tcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtt
tata