##gff-version 3
##sequence-region BBa_I3301 1 1814
BBa_I3301	.	CDS	76	1566	.	+	0	ID=BBa_C0082;Name=BBa_C0082
BBa_I3301	.	CDS	76	840	.	+	0	ID=annotation308352;Name=Tar (front);Parent=BBa_C0082
BBa_I3301	.	CDS	847	1527	.	+	0	ID=annotation308360;Name=Envz (back);Parent=BBa_C0082
BBa_I3301	.	tag	1528	1560	.	+	0	ID=annotation301146;Name=LVA;Parent=BBa_C0082
BBa_I3301	.	engineered_tag	1567	1591	.	+	0	ID=annotation2213997;Name=Help:Barcodes;Parent=BBa_C0082
BBa_I3301	.	point_mutation	498	499	.	+	0	ID=annotation300943;Name=A;Parent=BBa_C0082
BBa_I3301	.	terminator	1600	1679	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I3301	.	stem_loop	1611	1654	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I3301	.	engineered_region	1600	1679	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I3301	.	promoter	1737	1814	.	+	0	ID=BBa_R0083;Name=BBa_R0083
BBa_I3301	.	promoter	1781	1786	.	+	0	ID=annotation301224;Name=-35;Parent=BBa_R0083
BBa_I3301	.	sequence_feature	1773	1780	.	+	0	ID=annotation301223;Name=linker;Parent=BBa_R0083
BBa_I3301	.	point_mutation	1777	1777	.	+	0	ID=annotation306881;Name=A;Parent=BBa_R0083
BBa_I3301	.	non_covalent_binding_site	1749	1766	.	+	0	ID=annotation301221;Name=C1 OmpR;Parent=BBa_R0083
BBa_I3301	.	promoter	1804	1809	.	+	0	ID=annotation301226;Name=-10;Parent=BBa_R0083
BBa_I3301	.	ribosome_entry_site	58	69	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I3301	.	conserved_region	62	65	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I3301	.	promoter	1	49	.	+	0	ID=BBa_R1075;Name=BBa_R1075
BBa_I3301	.	promoter	15	20	.	+	0	ID=annotation309024;Name=-35;Parent=BBa_R1075
BBa_I3301	.	promoter	35	40	.	+	0	ID=annotation309020;Name=-10;Parent=BBa_R1075
BBa_I3301	.	terminator	1688	1728	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I3301	.	stem_loop	1695	1714	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I3301	.	stop_codon	1721	1721	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I3301	.	polyA_site	1715	1728	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I3301	.	engineered_region	1688	1728	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_I3301
ttaaatttcctcttttcaggccggaataactccctataatgcgccaccatactagagaaagaggagaaatactagatga
ttaaccgtatccgcgtagtcacgctgttggtaatggtgctgggggtattcgcactgttacagcttatttccggcagtct
gtttttttcttcccttcaccatagccagaagagctttgtggtttccaatcaattacgggaacagcagggcgagctgacg
tcaacctgggatttaatgctgcaaacgcgcattaacctgagtcgttcagcggtacggatgatgatggattcctccaatc
aacaaagtaacgccaaagttgaattgctcgatagcgccaggaaaacattggcgcaggcagcgacgcattataaaaaatt
caaaagcatggcaccgttacctgaaatggtcgctaccagtcgtaatattgatgaaaaatataaaaactattacacagcg
ttaactgaactgattgattatctcgattatggcaatactggagcttatttcgctcagccaacccagggaatgcaaaatg
caatgggcgaagcgtttgctcagtacgccctcagcagtgaaaaactgtatcgcgatatcgtcactgacaacgcagatga
ttaccgatttgcccagtggcaactggcggttatcgcgctggtggtggtattgattctgctggtggcgtggtacggcatt
cgccgtatgttgcttactccgctggcaaaaattattgctcacattcgcgaaatcgccggtggtaacctggcgaataccc
tgaccattgacgggcgcagtgaaatgggcgacctggcgcagagcgtttcacatatggcggctggtgttaagcaactggc
ggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgactgag
atgatgagcgagcaggatggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgccatcattgagcagt
ttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtgattgc
tgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcg
atcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatggcaatggctggatcaaagtcagcagcggaacgg
agccgaatcgcgcctggttccaggtggaagatgacggtccgggaattgcgccggaacaacgtaagcacctgttccagcc
gtttgtccgcggcgacagtgcgcgcaccattagcggcacgggattagggctggcaattgtgcagcgtatcgtggataac
cataacgggatgctggagcttggcaccagcgagcggggcgggctttccattcgcgcctggctgccagtgccggtaacgc
gggcgcagggcacgacaaaagaaggggctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgcta
gtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgtt
gtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtc
ccttgcatttacattttgaaacatctatagcgatctcttgagttggattattctgcatttttggggagaatggact