##gff-version 3
##sequence-region BBa_I3210 1 3279
BBa_I3210	.	ribosome_entry_site	58	69	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I3210	.	conserved_region	62	65	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I3210	.	ribosome_entry_site	1582	1593	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I3210	.	conserved_region	1586	1589	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I3210	.	promoter	1	49	.	+	0	ID=BBa_R1075;Name=BBa_R1075
BBa_I3210	.	promoter	15	20	.	+	0	ID=annotation309024;Name=-35;Parent=BBa_R1075
BBa_I3210	.	promoter	35	40	.	+	0	ID=annotation309020;Name=-10;Parent=BBa_R1075
BBa_I3210	.	CDS	1600	3117	.	+	0	ID=BBa_C0083;Name=BBa_C0083
BBa_I3210	.	tag	3079	3111	.	+	0	ID=annotation300993;Name=LVA;Parent=BBa_C0083
BBa_I3210	.	point_mutation	1600	1600	.	+	0	ID=annotation300982;Name=G;Parent=BBa_C0083
BBa_I3210	.	point_mutation	2256	2256	.	+	0	ID=annotation300986;Name=C;Parent=BBa_C0083
BBa_I3210	.	point_mutation	2724	2724	.	+	0	ID=annotation300988;Name=G;Parent=BBa_C0083
BBa_I3210	.	CDS	1600	3078	.	+	0	ID=annotation300981;Name=AspA;Parent=BBa_C0083
BBa_I3210	.	engineered_tag	3118	3142	.	+	0	ID=annotation2213998;Name=Help:Barcodes;Parent=BBa_C0083
BBa_I3210	.	point_mutation	2187	2187	.	+	0	ID=annotation300983;Name=C;Parent=BBa_C0083
BBa_I3210	.	point_mutation	2598	2598	.	+	0	ID=annotation300987;Name=G;Parent=BBa_C0083
BBa_I3210	.	point_mutation	2223	2223	.	+	0	ID=annotation300984;Name=G;Parent=BBa_C0083
BBa_I3210	.	terminator	3151	3230	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I3210	.	engineered_region	3151	3230	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I3210	.	stem_loop	3162	3205	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I3210	.	terminator	1325	1365	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I3210	.	polyA_site	1352	1365	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I3210	.	engineered_region	1325	1365	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I3210	.	stop_codon	1358	1358	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I3210	.	stem_loop	1332	1351	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I3210	.	terminator	3239	3279	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I3210	.	polyA_site	3266	3279	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I3210	.	engineered_region	3239	3279	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I3210	.	stop_codon	3272	3272	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I3210	.	stem_loop	3246	3265	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I3210	.	terminator	1237	1316	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I3210	.	engineered_region	1237	1316	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I3210	.	stem_loop	1248	1291	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I3210	.	promoter	1374	1573	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_I3210	.	CDS	1374	1461	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_I3210	.	start_codon	1546	1546	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_I3210	.	engineered_region	1374	1573	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_I3210	.	non_covalent_binding_site	1462	1499	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_I3210	.	promoter	1534	1539	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_I3210	.	non_covalent_binding_site	1539	1573	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_I3210	.	promoter	1510	1515	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_I3210	.	CDS	76	1203	.	+	0	ID=BBa_C0012;Name=BBa_C0012
BBa_I3210	.	engineered_region	76	1203	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_I3210	.	tag	1165	1203	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_I3210	.	engineered_tag	1204	1228	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_I3210	.	CDS	76	1203	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
>BBa_I3210
ttaaatttcctcttttcaggccggaataactccctataatgcgccaccatactagagaaagaggagaaatactagatgg
tgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaa
ccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgc
gtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgc
aaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgt
cgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgac
caggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatca
acagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgc
gctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaa
attcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagg
gcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggct
gcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccacc
atcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagg
gcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgc
gttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactac
gctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctca
gtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcg
ggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcag
ctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggca
ccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactaga
gaaagaggagaaatactagatgtgtttaaagcaaatcattggcagcttgaaaaagaaggttcacatgtcaaacaacatt
cgtatcgaagaagatctgttgggtaccagggaagttccagctgatgcctactatggtgttcacactctgagagcgattg
aaaacttctatatcagcaacaacaaaatcagtgatattcctgaatttgttcgcggtatggtaatggttaaaaaagccgc
agctatggcaaacaaagagctgcaaaccattcctaaaagtgtagcgaatgccatcattgccgcatgtgatgaagtcctg
aacaacggaaaatgcatggatcagttcccggtagacgtctaccagggcggcgcaggtacttccgtaaacatgaacacca
acgaagtgctggccaatatcggtctggaactgatgggtcaccaaaaaggtgaatatcagtacctgaacccgaacgacca
tgttaacaaatgtcagtccactaacgacgcctacccgaccggtttccgtatcgcagtttactcttccctgattaagctg
gtagatgcgattaaccaactgcgtgaaggctttgaacgtaaagctgtcgaatttcaggacatcctgaaaatgggtcgta
cccagctgcaagacgcagtaccgatgaccctcggtcaggaatttcgcgctttcagcatcctgctgaaagaagaagtgaa
aaacatccaacgtaccgctgaactgctgctggaagttaaccttggtgcaacagcaatcggtactggtctgaacacgccg
aaagagtactctccgctggcagtgaaaaaactggctgaagttactggcttcccatgcgtaccggctgaagacctgatcg
aagcgacctctgactgcggcgcttatgttatggttcacggcgcgctgaaacgcctggctgtgaagatgtccaaaatctg
taacgacctgcgcttgctctcttcaggcccacgtgccggcctgaacgagatcaacctgccggaactgcaagcgggctct
tccatcatgccagctaaagtaaacccggttgttccggaagtggttaaccaggtatgcttcaaagtcatcggtaacgaca
ccactgttaccatggcagcagaagcaggtcagctgcaattgaacgttatggagccggtcattggccaggccatgttcga
atccgttcacattctgaccaacgcttgctacaacctgctggaaaaatgcattaacggcatcactgctaacaaagaagtg
tgcgaaggttacgtttacaactctatcggtatcgttacttacctgaacccgttcatcggtcaccacaacggtgacatcg
tgggtaaaatctgtgccgaaaccggtaagagtgtacgtgaagtcgttctggaacgcggtctgttgactgaagcggaact
tgacgatattttctccgtacagaatctgatgcacccggcttacaaagcaaaacgctatactgatgaaagcgaacaggct
gcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatca
aataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagt
cacactggctcaccttcgggtgggcctttctgcgtttata