##gff-version 3
##sequence-region BBa_I3112 1 2234
BBa_I3112	.	terminator	1024	1103	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I3112	.	engineered_region	1024	1103	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I3112	.	stem_loop	1035	1078	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I3112	.	engineered_region	1336	2097	.	+	0	ID=BBa_E0022;Name=BBa_E0022
BBa_I3112	.	CDS	1336	2097	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_I3112	.	point_mutation	1404	1404	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_I3112	.	tag	2054	2091	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
BBa_I3112	.	stop_codon	2092	2097	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_I3112	.	engineered_region	1336	2097	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
BBa_I3112	.	CDS	76	990	.	+	0	ID=BBa_C0080;Name=BBa_C0080
BBa_I3112	.	CDS	76	951	.	+	0	ID=annotation308191;Name=araC;Parent=BBa_C0080
BBa_I3112	.	engineered_tag	991	1015	.	+	0	ID=annotation2214004;Name=Help:Barcodes;Parent=BBa_C0080
BBa_I3112	.	tag	952	984	.	+	0	ID=annotation308196;Name=LVA;Parent=BBa_C0080
BBa_I3112	.	terminator	1112	1152	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I3112	.	polyA_site	1139	1152	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I3112	.	stem_loop	1119	1138	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I3112	.	engineered_region	1112	1152	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I3112	.	stop_codon	1145	1145	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I3112	.	terminator	2106	2185	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I3112	.	engineered_region	2106	2185	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I3112	.	stem_loop	2117	2160	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I3112	.	promoter	1	49	.	+	0	ID=BBa_R1075;Name=BBa_R1075
BBa_I3112	.	promoter	15	20	.	+	0	ID=annotation309024;Name=-35;Parent=BBa_R1075
BBa_I3112	.	promoter	35	40	.	+	0	ID=annotation309020;Name=-10;Parent=BBa_R1075
BBa_I3112	.	promoter	1161	1309	.	+	0	ID=BBa_R0080;Name=BBa_R0080
BBa_I3112	.	promoter	1273	1278	.	+	0	ID=annotation308601;Name=-35;Parent=BBa_R0080
BBa_I3112	.	non_covalent_binding_site	1203	1232	.	+	0	ID=annotation301458;Name=c-amp1;Parent=BBa_R0080
BBa_I3112	.	non_covalent_binding_site	1164	1189	.	+	0	ID=annotation301456;Name=c-amp2;Parent=BBa_R0080
BBa_I3112	.	operator	1166	1204	.	+	0	ID=annotation301457;Name=araO1;Parent=BBa_R0080
BBa_I3112	.	promoter	1296	1301	.	+	0	ID=annotation308602;Name=-10;Parent=BBa_R0080
BBa_I3112	.	non_covalent_binding_site	1233	1261	.	+	0	ID=annotation301462;Name=ara1 and ara2;Parent=BBa_R0080
BBa_I3112	.	terminator	2194	2234	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I3112	.	polyA_site	2221	2234	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I3112	.	stem_loop	2201	2220	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I3112	.	engineered_region	2194	2234	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I3112	.	stop_codon	2227	2227	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I3112	.	ribosome_entry_site	58	69	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I3112	.	conserved_region	62	65	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I3112	.	ribosome_entry_site	1318	1329	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I3112	.	conserved_region	1322	1325	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_I3112
ttaaatttcctcttttcaggccggaataactccctataatgcgccaccatactagagaaagaggagaaatactagatgg
ctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggc
caatggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcag
ggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatc
actacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatg
gcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctg
tttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgt
tactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacat
cagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgccgtcgcgtctgtca
catcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagccaggcgaagctgcttt
tgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatt
taaaaaatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttg
tcagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccag
gcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctac
tagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcgtaacaaaagtgtctataatcac
ggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcgg
atcctacctgacgctttttatcgcaactctctactgtttctccattactagagaaagaggagaaatactagatggtgag
caagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagc
gtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccg
tgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagca
cgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaag
acccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacg
gcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacgg
catcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacc
cccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacg
agaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagag
gcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctca
gtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcg
ggtgggcctttctgcgtttata