##gff-version 3
##sequence-region BBa_I2057 1 862
BBa_I2057	.	engineered_region	20	862	.	+	0	ID=BBa_J04650;Name=BBa_J04650
BBa_I2057	.	CDS	20	700	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J04650
BBa_I2057	.	CDS	20	694	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_I2057	.	engineered_tag	701	725	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_I2057	.	terminator	734	813	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J04650
BBa_I2057	.	engineered_region	734	813	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I2057	.	stem_loop	745	788	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I2057	.	terminator	822	862	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J04650
BBa_I2057	.	stem_loop	829	848	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I2057	.	polyA_site	849	862	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I2057	.	stop_codon	855	855	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I2057	.	engineered_region	822	862	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I2057	.	ribosome_entry_site	1	13	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_I2057	.	engineered_region	1	13	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_I2057	.	ribosome_entry_site	7	10	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_I2057	.	ribosome_entry_site	1	13	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
>BBa_I2057
tcacacaggaaagtactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggt
tccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactga
aagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgt
taaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttc
gaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtg
gtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtaccc
ggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaa
accacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccaca
acgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtg
ctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatct
gttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata