##gff-version 3
##sequence-region BBa_I2056 1 903
BBa_I2056	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I2056	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I2056	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I2056	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I2056	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I2056	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I2056	.	engineered_region	61	903	.	+	0	ID=BBa_J04650;Name=BBa_J04650
BBa_I2056	.	terminator	863	903	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J04650
BBa_I2056	.	stem_loop	870	889	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I2056	.	stop_codon	896	896	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I2056	.	polyA_site	890	903	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I2056	.	engineered_region	863	903	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I2056	.	terminator	775	854	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J04650
BBa_I2056	.	stem_loop	786	829	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I2056	.	engineered_region	775	854	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I2056	.	CDS	61	741	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J04650
BBa_I2056	.	CDS	61	735	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_I2056	.	engineered_tag	742	766	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
>BBa_I2056
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatggcttcctccgaagacg
ttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaagg
tgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggac
atcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgt
ccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctc
cctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaa
aaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtc
tgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccggg
tgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgct
gaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaa
acgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacact
ggctcaccttcgggtgggcctttctgcgtttata