##gff-version 3
##sequence-region BBa_I2028 1 1828
BBa_I2028	.	promoter	1	49	.	+	0	ID=BBa_R1075;Name=BBa_R1075
BBa_I2028	.	promoter	15	20	.	+	0	ID=annotation309024;Name=-35;Parent=BBa_R1075
BBa_I2028	.	promoter	35	40	.	+	0	ID=annotation309020;Name=-10;Parent=BBa_R1075
BBa_I2028	.	CDS	76	837	.	+	0	ID=BBa_C0071;Name=BBa_C0071
BBa_I2028	.	tag	799	831	.	+	0	ID=annotation306523;Name=LVA;Parent=BBa_C0071
BBa_I2028	.	engineered_tag	838	862	.	+	0	ID=annotation2213995;Name=Help:Barcodes;Parent=BBa_C0071
BBa_I2028	.	CDS	76	798	.	+	0	ID=annotation301192;Name=rhlR;Parent=BBa_C0071
BBa_I2028	.	engineered_region	1067	1828	.	+	0	ID=BBa_E0022;Name=BBa_E0022
BBa_I2028	.	tag	1785	1822	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
BBa_I2028	.	engineered_region	1067	1828	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
BBa_I2028	.	stop_codon	1823	1828	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_I2028	.	point_mutation	1135	1135	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_I2028	.	CDS	1067	1828	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_I2028	.	terminator	959	999	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I2028	.	stem_loop	966	985	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I2028	.	stop_codon	992	992	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I2028	.	polyA_site	986	999	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I2028	.	engineered_region	959	999	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I2028	.	terminator	871	950	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I2028	.	stem_loop	882	925	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I2028	.	engineered_region	871	950	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I2028	.	ribosome_entry_site	58	69	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I2028	.	conserved_region	62	65	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I2028	.	promoter	1008	1060	.	+	0	ID=BBa_R0071;Name=BBa_R0071
BBa_I2028	.	non_covalent_binding_site	1008	1027	.	+	0	ID=annotation297102;Name=RhlR;Parent=BBa_R0071
BBa_I2028	.	promoter	1049	1054	.	+	0	ID=annotation297104;Name=-10;Parent=BBa_R0071
BBa_I2028	.	promoter	1026	1031	.	+	0	ID=annotation297103;Name=-35;Parent=BBa_R0071
BBa_I2028	.	start_codon	1060	1060	.	+	0	ID=annotation297105;Name=start;Parent=BBa_R0071
>BBa_I2028
ttaaatttcctcttttcaggccggaataactccctataatgcgccaccatactagagaaagaggagaaatactagatga
ggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgtt
cgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacc
cggccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtgg
atccggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgct
ctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtg
ctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcg
agttgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacg
cgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtg
aacttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgg
gtctcatcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactaga
gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc
tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcctgtgaaatctggcagtt
accgttagctttcgaattggctaaaaagtgttctactagatggtgagcaagggcgaggagctgttcaccggggtggtgc
ccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccaccta
cggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacc
tggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggct
acgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacac
cctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaac
tacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaaca
tcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccga
caaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttc
gtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttag
tagcttaataa