##gff-version 3
##sequence-region BBa_I20251 1 903
BBa_I20251	.	engineered_region	742	766	.	+	0	ID=annotation1941302;Name=barcode
BBa_I20251	.	engineered_region	61	741	.	+	0	ID=annotation1941301;Name=BBa_E1010
BBa_I20251	.	engineered_region	1	54	.	+	0	ID=annotation1941296;Name=BBa_R0040
BBa_I20251	.	stop_codon	896	896	.	+	0	ID=annotation1941308;Name=stop
BBa_I20251	.	promoter	20	25	.	+	0	ID=annotation1941297;Name=-35
BBa_I20251	.	stem_loop	870	889	.	+	0	ID=annotation1941306;Name=T7 TE
BBa_I20251	.	CDS	61	735	.	+	0	ID=annotation1941300;Name=mrfp1
BBa_I20251	.	stem_loop	786	829	.	+	0	ID=annotation1941304;Name=stem_loop
BBa_I20251	.	polyA_site	890	903	.	+	0	ID=annotation1941307;Name=polya
BBa_I20251	.	promoter	43	48	.	+	0	ID=annotation1941299;Name=-10
BBa_I20251	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1941295;Name=TetR 1
BBa_I20251	.	engineered_region	775	854	.	+	0	ID=annotation1941303;Name=BBa_B0010
BBa_I20251	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1941298;Name=TetR 2
BBa_I20251	.	engineered_region	863	903	.	+	0	ID=annotation1941305;Name=BBa_B0012
>BBa_I20251
gaattgtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagtgcggccgctcta
gatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgag
ttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtc
cgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacat
cccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgtt
accgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccg
acggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaa
aggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaa
aaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcg
ttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctact
agagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacg
ctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttataatgcag